山竹果皮总黄酮富集工艺研究

研究D101大孔树脂富集山竹果皮总黄酮的工艺。通过静态、动态相结合的方法,确定D101大孔树脂富集的最佳工艺参数。结果表明,D101大孔树脂对总黄酮动态解析附的效果好于静态,动态解析附的最佳条件为:以流速2 m L/min上样30 m L 2 mg/m L的提取物,且用60 m L 70%乙醇以2 m L/min的流速洗脱,此条件下提取物总黄酮含量由原先的57.8 mg/m L增加到174.2 mg/m L。     

Effects of Garcinia cambogia extract on serum sex hormones in overweight subjects

(-) Hydroxycitric acid (HCA), an active ingredient extracted from the Garcinia cambogia fruit rind, has been commonly used as a dietary supplement for weight management. Given the controversy over HCA related testicular toxicity in animal studies, we investigated changes in serum sex hormones levels as an extension of our previous double-blind placebo-controlled trial in human subjects, in which 44 participants received either G. cambogia extract (1667.3 mg/day equivalent to 1000 mg HCA/day) or placebo for 12 weeks. Compared to the placebo group, administration of the extract did not significantly alter the serum testosterone, estrone, and estradiol levels. Similarly, hematology, serum triacylglycerol and serum clinical pathology parameters did not reveal any significant adverse effects. The results of this preliminary investigation indicate that ingestion of G. cambogia extract at dose levels commonly recommended for human use does not affect serum sex hormone levels and blood parameters.     

影响山竹果皮过氧化氢酶活性因素的研究

以山竹果皮中的过氧化氢酶(CAT)为研究对象,对其酶的活性变化及其特性的研究结果表明,山竹果皮中过氧化氢酶的最适温度为30℃,最适pH值为7.2。甲醇和乙醇对山竹果皮中过氧化氢酶活性的影响均表现为低浓度对该酶具有激活作用,高浓度转为抑制作用。金属离子Cu2+对该酶有激活作用,而Na+则对该酶有一定程度的抑制作用。     

热带植物山竹在美容护肤领域应用的研究进展

山竹是一种常见的热带植物,富含黄酮类、多酚、蛋白质和脂类。因其良好的抗氧化活性,以及抗菌,抗炎及抗过敏的功效,日益受到医药学界的重视,其提取物在国外已产业化,并应用于食品及化妆品行业。介绍了目前国内外对山竹的化学成分、抗氧化活性、抗菌活性、抗炎及抗过敏、增强免疫及抗肿瘤等方面的研究进展,旨在加深对山竹的诸多有效化学组分研究,并充分结合临床,尽快用于治疗和预防相关疾病。     

多花山竹子组培初代培养技术研究

旨在为多花山竹子组培快繁体系建立提供参考。通过观察对比试验法、完全随机化法正交试验设计、运用方差分析、显著性检验、LSD多重比较,探讨多花山竹子组培的外植体最佳消毒时间控制和最适初代培养基类型与激素浓度配方。试验表明：多花山竹子组培的外植体消毒时间为2 min时其污染率最高、死亡率与存活率较低,消毒时间为6 min时其污染率和死亡率居中、存活率最高,消毒时间为10 min时其污染率与存活率最低、死亡率最高;以MS和B5作为多花山竹子组培的初代培养基培养效果较好,若对MS和B5分别添加不同浓度的6-BA、NAA ,对其外植体不定芽的诱导促进作用不同,MS的最佳配比为6-BA 1.6 mg/L+NAA 0.9 mg/L,B5的最佳配比为6-BA 1.6 mg/L+NAA 0.6 mg/L。考虑各因素和激素交互作用,综合认为其外植体消毒时间控制6 min、初代培养基类型与激素浓度配方为MS+6-BA 1.6 mg/L+NAA 0.9 mg/L时培养效果最好。     

大果藤黄乙醇提取物的降血脂与抗氧化性

为充分挖掘大果藤黄的药用功能,利用超声波辅助提取大果藤黄果的活性成分,通过两性霉素B-细胞模型观察大果藤黄乙醇提取物(EG)对细胞内胆固醇生物合成的抑制作用,检测中国仓鼠卵细胞(CHO)的存活率;采用灌胃血脂康和不同剂量的EG建立高脂大鼠模型,测定血清、肝脏和心脏中的胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白以及磷脂含量。结果表明:EG能明显抑制胆固醇生物合成,不同治疗组能明显降低血清、肝脏和心脏中的胆固醇(TC)、甘油三酯(TG)、低密度胆固醇(LDH)的含量和增加高密度胆固醇(HDL)和磷脂的含量;同时,EG也能明显降低肝组织中的MDA含量和提高SOD、CAT和GSH含量。大果藤黄的乙醇提取物具有较好的降血脂和抗氧化效果。     

Cytotoxic alkoxylated xanthones from the resin of Garcinia hanburyi

Three new xanthones, garcinolic acid (1), 10α-ethoxy-9,10-dihydromorellic acid (2), and 10α-ethoxy-9,10-dihydrogambogenic acid (3), along with six known compounds were isolated from the resin of Garcinia hanburyi. These compounds were tested for their cytotoxicities against A549, HCT116, SK-BR-3 and HepG2, and showed high inhibitory effects on the cell lines.     

从泰国进境山竹上截获藤黄树链蚧Asterolecanium garciniae (Hemiptera: Asterolecaniidae)

本研究描述了藤黄树链蚧Asterolecanium garciniae的田间形态特征和显微形态特征;给出了结构测量值;提供了生态照片、显微照片和显微绘图;评述了与近似种A.javae、A.russellae和A.sumatrae的区别;提供了寄主和分布,首次报道了新的分布地曼谷;藤黄树链蚧为我国口岸首次截获,该研究结果可为链蚧的检疫鉴定及该种蚧虫的截获鉴定提供参考。     

Collection and Characterisation of Malabar Tamarind [Garcinia cambogia (Gaertn.) Desr.]

A collaborative crop specific exploration and collection mission to collect the germplasm and to study the population size of male and female/hermaphrodite trees of Malabar tamarind [Garcinia cambogia (Gaertn.) Desr.] in the area of its diversity was undertaken during July 2002 in Kerala and Karnataka. A total of 56 accessions of Malabar tamarind were collected. Two collections of Malabar tamarind were found to be very specific because of the uncommon fruit colour, which is pinkish red. All the collected accessions are grown at National Bureau of Plant Genetic Resources (NBPGR) Regional Station, Thrissur for characterisation and conservation. Extensive range of variability was found in fruit colour, shape, size and nature of branching and canopy of trees. Characterisation of 13 fruit and five seed characters was done for 51 accessions. The variability was found to be maximum for nipple length (74.8%) and minimum for fruit girth (12.8%). Two promising accessions were identified based on mean fruit weight (161 g) in IC 354028 and mean rind thickness (15 mm) and mean rind weight (125 g) in IC 354019.     

山枇杷基因组DNA的提取及自然群体内ISSR遗传多样性分析

为探究山枇杷基因组DNA提取方法及群体内个体之间的遗传多样性,以采自福建省戴云山脉九仙山山枇杷(Garcinia multiflora)自然群体的41份叶片样品为材料,比较2种改良十六烷基三甲基溴化铵法(CTAB)对其基因组DNA的提取效果,并通过简单序列重复区间—聚合酶链式反应(ISSR-PCR)扩增反应体系分析其群体内遗传多样性。结果表明,CTAB-2法通过适当提高CTAB浓度,添加聚乙烯吡咯烷酮(PVP)和酚—氯仿—异戊醇,获得的基因组DNA纯度和质量较好;筛选的11条ISSR引物共扩增85个条带,其中多态性条带61条,占71.76%;NTSYS 2.10e软件设定阈值(GS)为0.78,该山枇杷样品归为4个类群,样品遗传相似系数介于0.588-0.929,平均为0.779,说明该群体的遗传基础较宽;利用STRUCTURE 2.2软件将样品分为4个类群,结合后验概率(Q值)分布表表明部分个体之间存在较丰富的遗传信息交流。