多花山竹子组培初代培养技术研究

旨在为多花山竹子组培快繁体系建立提供参考。通过观察对比试验法、完全随机化法正交试验设计、方差分析、显著性检验、LSD多重比较,探讨多花山竹子组培的外植体最佳消毒时间控制和最适初代培养基类型与激素浓度配方。试验表明:多花山竹子组培的外植体消毒时间为2 min时其污染率最高、死亡率与存活率较低。消毒时间为6 min时其污染率和死亡率居中、存活率最高。消毒时间为10 min时其污染率与存活率最低、死亡率最高。以MS和B5作为多花山竹子组培的初代培养基培养效果较好,若对MS和B5分别添加不同浓度的6-BA和NAA,对其外植体不定芽的诱导促进作用不同,MS的最佳配比为6-BA 1.6 mg/L+NAA 0.9 mg/L,B5的最佳配比为6-BA 1.6 mg/L+NAA 0.6 mg/L。综上,外植体消毒时间为6 min、MS+6-BA 1.6 mg/L+NAA 0.9 mg/L的培养效果最好。

The technique of bud propagation in tissue culture of Garcinia multiflora Champ

The study aims at providing references for the rapid propagation system establishment of tissue culture of Garcinia multiflora Champ. By means of observing the comparative experiments, completely randomized orthogonal experiment design, analysis of variance (ANOVA), significance test and LSD multiply comparative analysis, the control of the most optimal sterilization time, the optimal primary culture medium type and hormone concentration formula were discussed. The results showed that when the sterilization time of explants was 2 min, the pollution rate was the highest, while the mortality rate and survival rate were relatively low. When the sterilization time was 6 min, the pollution rate and mortality rate were medium, while the survival rate was the highest. When the time was 10 min, the pollution rate and survival rate were the lowest, while the mortality rate was the highest. MS and B5 had better effect on the primary culture medium of Garcinia multiflora Champ. If MS and B5 were respectively added with different concentrations of 6-BA and NAA, the induction effect of adventitious buds of explants was different. The maximum proportion of MS was 6-BA 1.6 mg/L + NAA 0.9 mg/L, and the best ratio of B5 was 6-BA 1.6 mg/L + NAA 0.6 mg/L. Taken together, the optimum sterilization time of explants is 6 min, MS + 6-BA 1.6 mg/L + NAA 0.9 mg/L has the best culture effect.