高产优质多抗小麦新品种秦农578的选育

系统介绍了小麦新品种秦农578的选育历程、特征特性、配套技术等方面情况,并结合育种实践对黄淮麦区小麦育种几个关键性问题进行了探讨.     

miR-142-3p在MCF-7细胞中靶向调节AKT pathway活性机理

为探究miR-142-3p在MCF-7细胞中作用机理,采用RNA免疫共沉淀技术和双荧光素酶报告基因技术筛选及验证miR-142-3p作用靶基因;蛋白质免疫印迹技术验证靶基因及其介导的PI3K-AKT-mTOR信号通路蛋白表达量及通路活性;应用实时荧光定量PCR验证靶基因PTEN对miR-142-3p调节关系。结果表明,miR-142-3p靶向调节AKT和PTEN表达;miR-142-3p过表达组中PI3K-AKT-mTOR通路活性显著降低;miR-142-3p抑制组中PI3K-AKT-mTOR通路活性显著上升;抑制PTEN表达显著提高miR-142-3p表达量。因此miR-142-3p靶向调节AKT和PTEN表达继而抑制PI3K-AKT-mTOR信号通路活性,PTEN与miR-142-3p存在调节关系。     

优质抗稻瘟病杂交水稻新品种绵5优142

绵5优142是湖北省恩施自治州农业科学院用抗稻瘟病、米质优良的早中熟恢复系恩恢142与绵5A配组育成的优质、抗稻瘟病、早熟中籼新品种。2011年4月通过湖北省农作物品种审定委员会审定。     

高粱新品种吉杂142的选育与栽培

高粱新品种吉杂142高产、稳产,适应性广,抗逆性强,抗叶病,抗倒伏,抗蚜虫,中抗高粱丝黑穗病;株型好,叶片较窄并上冲,适于密植,适于机械化收获。阐述了该品种的亲本来源与选育经过,总结了其主要的特征特性,包括植物学、生物学、抗性、产量等,并简述了其栽培、繁育技术与适应的区域范围,为该品种的推广应用提供参考依据。     

国审广适高产小麦新品种秦农142选育及栽培技术

秦农142是宝鸡市农科所采用改良系统优选法成功选育的抗病、广适、早熟、优质、高产、稳产小麦新品种。2004年以来先后参加了国家黄淮南片春水组区域试验和生产试验、陕西省中肥组区域试验和水地生产试验,在各级多点试验、示范中表现突出,增产显著,名列前茅。该品种一般单产7500～9000kg/hm2,高产潜力可达9750kg/hm2。2005年通过国家和陕西省审定。     

Effect of miR-142-3p on Lactation in Diary Goat Mammary Epithelial Cells

To determine the regulative role of miR-142-3p in lactation of dairy goat mammary epithelia cells (DGMECs),we selected the DGMECs in lactating dairy goat for material,used liposomal transfection techniques to silence miR-142-3p expression,and used Real-time quantitative PCR,Western blotting and Assay Kits to explore the changes of lactation after miR-142-3p inhibition.The results showed that when miR-142-3p was inhibited,the cell proliferation ability of DGMECs was increased,the secretion amount of β-casein and triglyceride were up-regulated.So we concluded that miR-142-3p could influence the lactation function of DGMECs,such as cell proliferation,β-casein and triglyceride secretion,by inhibiting the function of its target genes.     

Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein

MicroRNAs (miRNAs) are a group of small,non-coding RNA molecules about 22 nucleotides to regulate a wide variety of important biological processes,including cell proliferation,differentiation,apoptosis as well as the progression of tumors.Liposome transfection was used to detect the expression of miR-142-3p in human mammary epithelial cells,the effects of miR-142-3p on the cell proliferation,apoptosis and milk protein synthesis were detected by Real-time PCR,Western blotting,cell proliferation analysis.The results indicated that after miR-142-3p being silenced,prolactin receptor (PRLR) protein was increased,at the same time the expressions of related pathways protein AKT,mTOR,STAT5 and cyclinD1 were increased,the ability of cell proliferation was increased.The results suggested that in human mammary epithelial cells,the silence of miR-142-3p could increase the expression of PRLR protein,miR-142-3p could promote the synthesis of milk protein and increase the proliferation of mammary epithelial cells by regulating related pathways proteins AKT,mTOR,STAT5 and cyclinD1.     

朊病毒病生物标记microRNA-142-3p可视化快速检测

为了更快捷、方便的检测朊病毒病,根据已有报道确定micro RNA-144-3p作为朊病毒病的生物标记,通过mi RBase数据库公布的micro RNA-142-3p的核酸序列,设计特异性标记有生物素的俘获探针和标记有FAM的检测探针,从而建立了纳米金偶联核酸试纸条的高效,快捷,可视化检测方法。结果表明:该检测方法具有良好的特异性和较高的灵敏度(0.005 nmol·L-1)。该检测试纸条的建立在朊病毒病预防,控制,诊断过程中具有一定实际意义。