Vegetative propagation of Colutea istria Mill. from leafy stem cuttings

This study investigates the optimal conditions for the vegetative propagation of the multipurpose leguminous shrub Colutea istria from leafy stem cuttings. The conditions examined included 1) the origin of the cutting (apical, medial or basal), 2) indole-3-butyric acid (IBA) treatment (0, 50, 200 or 400 mg/l), 3) the relative humidity of the propagation environment (high or low), 4) wounding base of cutting, and 5) the season in which the cutting was taken. The rooting ability of the cuttings was significantly influenced by all these factors. Winter cuttings obtained from the middle and base of the branch showed a greater rooting capacity than those harvested from the apical area, while autumn cuttings harvested from the middle and apical areas of the branch showed a greater rooting capacity than those harvested from the base. Winter cuttings showed the best rooting capacity than autumn cuttings. IBA treatment (200 mg/l), high humidity (98-100%), and wounding increased the rooting capacity of the cuttings. The sprout of new leaves was higher in basal cuttings, while IBA and wounding also significantly enhanced the root number of rooted cuttings. A method for vegetatively propagating C. istria plants is given.     

植物样品采集·制备·储存及消解过程中存在问题分析

结合目前单位所承接的植物样品检测任务的实际情况,分析了植物样品的野外采集、加工制备及消解过程中会遇到的几个问题,为准确测定植物样品中重金属元素以及客观评价植物重金属污染提供了保障。     

早胜牛的采精及细管冻精制作技术要点

近年来，伴随我国畜牧业蓬勃发展的同时，为了更大限度的提高母畜的受胎率和充分利用优秀种畜的基因，人工授精技术得到了不断完善和发展，并在全国各地广泛推广和应用。本文结合实践经验总结出的一些技术措施，阐述了对早胜牛这一地方优良种质资源进行保种和开发利用的重要意义，同时指出人工授精技术和低温冷冻保存精液技术在早胜牛上的实践，对于进一步开发利用培育优质高产肉牛新品种，促进肉牛产业高质量发展具有非常重要的现实意义。通过早胜牛种公牛采精的全过程以及细管冻精制作要点的详细讲解，低温冷冻保存精液技术，可更经济、可靠地实现家畜品种资源的保护，让优良基因长时间保存使得后续研究需要得到保障。     

四川及藏东南荞麦资源的调查研究

本文叙述了2010年中国农业科学院农业生物资源考察队对四川及藏东南荞麦资源的详细调查结果.考察队共调查了40个乡镇的120个村,360余个村小组,共获得荞麦资源88份,其中苦荞资源64份,甜荞资源20份,野生荞麦资源4份.当地少数民族认知有价值的荞麦资源分别是长锥大粒型资源、短锥皮薄型资源、抗病资源、抗虫资源、抗旱资源和抗寒资源等.     

高校图书馆保存本的改革

高校图书馆保存本必须改革。文章指出保存本存在的问题以及改革的必要性、措施和意义,改革的有效措施是变保存本为特色馆藏。     

基于SOFM神经网络的核心种质取样算法

介绍了核心种质和自组织特征映射网络的基本概念，使用自组织特征映射网络的自组织特性对种质性状数据进行了聚类，利用聚类的分组结果，提取核心种质，通过对核心种质的各种参数的检验并与完全随机取样和一般的系统聚类的对比。结果表明，SOFM的取样算法，是一种比较有效快捷的算法，可以在实际中加以应用。     

Identification of intra-accession genetic diversity in selfing crops using AFLP markers: implications for collection management

Germplasm conserved as seeds in genebanks requires regular regeneration. In this process, selection and genetic drift may cause loss of genetic diversity from accessions. In the case of selfing crops, separation of distinct lines into different accessions may be an efficient strategy to avoid these negative effects. In order to evaluate the applicability of this method for collection management, knowledge about the level of intra-accession genetic diversity is required. By means of AFLP analysis intra-accession variation was investigated in two cultivars, two landraces and two wild populations of ex situ conserved barley germplasm. In the total sample of 216 individuals analysed (36 per accession), 22 genotypes were observed based on 104 polymorphic loci. The number of genotypes detected ranged from 1 to 3 per accession, except for a Nepalese landrace that revealed 12 genotypes. An UPGMA cluster analysis grouped the genotypes unambiguously into the accession they belonged to and genotypes within accessions were generally found to be closely related. In order to determine the repeatability of the results obtained, 11 individuals belonging to 4 genotypes from the Nepalese landrace were scored for a second set of AFLP markers. Matrices of genetic distances calculated for the two AFLP datasets were found to be highly correlated (r = 0.9346, P < 0.001). Separation of genotypes into different accessions was considered a relevant option only for the Nepalese landrace. Analysis of molecular variance indicated that this accession could be well divided into 8 distinct lines. Further implications of the results for genebank practices are discussed.     

Genetic and economic aspects of marker-assisted reduction of redundancy from a wild potato germplasm collection

The wild potato germplasm collection of the series Acaulia, maintained at the Centre for Genetic Resources, The Netherlands, consists of 314 accessions. In previous investigations, 15 potential duplication groups with a total of 36 accessions were identified based on AFLP analysis of a limited sample per accession. In the present study, the potential redundancies, plus one additional accession, were studied with increased sample sizes to examine intra- and inter-accession variation more accurately, with the aim to reduce the size of the collection. No variation was observed within two potential duplication groups, whereas only limited differentiation among accessions was detected within seven groups, resulting in a total of 15 redundant accessions (nearly 5% of the collection). A cluster analysis of all the accessions of the collection showed that these nine groups each had a distinct identity. It was decided to maintain the accessions of the remaining six groups as separate entries based on the large differentiation observed among accessions and the absence of a clear identity. An analysis of molecular variance in the set of 37 accessions showed that 91% of the observed variation could be found among accessions. This variance component appeared unaffected when the set was analysed without the 15 redundant accessions. The invested costs to identify redundancies in the series Acaulia by AFLP analysis are estimated at k 57.3, whereas the savings achieved by reduction of the collection are estimated at k 21.0 per generation. However, a cost-benefit analysis should not only focus on the short-term return of investments, but should also consider the value of newly obtained data and information. These include taxonomic information about accessions, optimised sampling strategies, optimised regeneration procedures, additional data for core collection formation and more efficient utilisation of germplasm.     

节水型绿地中雨水的收集与利用

阐述了节水型绿地中雨水利用的可行性和必要性,探讨了雨水的收集、净化、存储与利用途径,以期为节水型绿地建设方面的研究提供帮助。     

数据采掘技术与信息服务

以信息服务为目的，较详细的介绍了数据采掘技术的过程、方法及工具，并对信息服务的流程、现代化手段和发展方向以及CIO在信息服务中的地位作用等作了简单的论述。