番茄耐弱光壮苗钙素配方的筛选试验

为提高番茄(Lycopersicon esculentum Mill)幼苗的耐弱光性,以自然光(CK,实测光通量密度为855.3～1033.6μmol·s-1·m-2)和50%遮光(CKz,422.2～524.6μmol·s-1·m-2)为对照,研究了50%遮光下全株喷施4种耐弱光壮苗钙素配方对番茄植株生长发育及产量的调控效应。结果表明:与CKz相比,50%遮光下全株喷施4种耐弱光壮苗钙素配方均可抑制番茄植株徒长,显著提高番茄幼苗茎粗、全株干重和壮苗指数,增强SOD、CAT和POD等保护酶活性,促进光合作用,显著增加单果重和单株产量,其中配方4效果最好,其平均单果重和单株产量比CKz提高了34.2%和31.7%,可作为番茄育苗的耐弱光钙素壮苗配方。     

南瓜发酵蛋糕的研制

以蛋糕粉为主要原料,添加南瓜粉,研制一种南瓜发酵蛋糕。选择南瓜粉、鸡蛋液、干酵母、糖浆为考察因素,通过单因素试验和正交试验,确定南瓜发酵蛋糕的最佳配方为南瓜粉19%、鸡蛋液56%、干酵母3%、糖浆25%。     

紫锥菊复合物对雏鸡人工感染传染性法氏囊病病毒的效果观察

本试验将240只海兰褐雏鸡随机分为8组,每组30只,分别为紫锥菊复合物高剂量组、中剂量组、低剂量组、中药对照组、西药对照组、疫苗对照组、阴性对照组、健康对照组,采用紫锥菊复合物,将试验鸡人工感染传染性法氏囊病病毒（IBDV）强毒,观察紫锥菊复合物对雏鸡的保护作用。结果表明：紫锥菊复合物可以减弱IBDV强毒对雏鸡免疫器官的损害,对传染性法氏囊病强毒感染雏鸡具有明显的保护作用。     

丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响

目的 探讨丹龙醒脑方对脑缺血再灌注模型大鼠海马区Notch信号通路相关蛋白Notch1、Notch2及Hes1表达的影响。方法 将80只雄性SD大鼠随机分为假手术组、模型组、丹龙醒脑方小剂量组（丹小组7.4 g/kg）、大剂量组（丹大组14.8 g/kg）,线栓法建立局灶性脑缺血再灌注大鼠模型,再灌注7 d取缺血侧海马组织。采用免疫组织化学法、免疫印迹法检测大鼠海马区Notch1、Notch2及Hes1蛋白表达。结果 与假手术组比较,各组Notch1、Notch2及Hes1蛋白表达显著升高（P<0.05,P<0.01）;与模型组比较,丹小组、丹大组Notch1、Notch2及Hes1蛋白表达显著升高（P<0.05,P<0.01）。结论 丹龙醒脑方能通过上调海马区Notch1、Notch2及Hes1蛋白表达水平,调节Notch信号转导通路,这可能是其促进脑缺血损伤后神经功能修复的机制之一。     

复方穿心莲对AA+肉鸡肠黏膜结构的影响

本试验旨在研究复方穿心莲对AA+肉鸡肠道黏膜结构的影响。将1日龄240只AA+肉仔鸡随机分为A、B、C、D 4组,试验组添加不同浓度的复方穿心莲,饲喂至42日龄,取7、35和42日龄肉鸡肠道组织,制成切片测量其绒毛长度、隐窝深度、肠壁厚度。结果表明,与空白组相比,A、B组肉鸡7、35和42日龄肠绒毛长度显著增加（P＜0.05）;隐窝深度显著降低（P＜0.05）;C组肉鸡35日龄肠壁厚度显著（P＜0.05）高于其它组。复方穿心莲能明显增加AA+肉鸡绒毛长度,降低隐窝深度和肠壁厚度。     

Effects of Xinkang recipe on myocardial miR-25-3p expression and SERCA2a activity in heart failure rats

AIM: To investigate the effects of Xinkang recipe on myocardial miR-25-3p expression and sarcoplasmic reticulum calcium ATPase 2a (SERCA2a) activity in heart failure rats. METHODS: Male SD rats were randomly divided into normal group, sham group, model group, Xinkang recipe group (Xinkang group), and captopril group. The heart failure rat model was induced by intraperitoneal injection of doxorubicin. Distilled water, Xinkang recipe and captopril were administrated by gastric gavage for 35 d, respectively. The indexes of cardiac function and plasma level of brain natriuretic peptide (BNP) were measured. The SERCA2a activity was determined by the inorganic phosphorus method. The myocardial protein expression of SERCA2a and phospholamban (PLB) was detected by Western blot. The myocardial expression of miR-25-3p was detected by stem-loop RT-qPCR. RESULTS: Cardiac output (CO), left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) in Xinkang group and captopril group were significantly higher while the plasma levels of BNP were significantly lower than those in model group (P<0.01). The myocardial expression levels of miR-25-3p in Xinkang group and captopril group were significantly lower while the myocardial protein le-vels of SERCA2a and PLB were significantly higher than those in model group (P<0.01). The SERCA2a/PLB ratio and SERCA2a activity in Xinkang group were significantly higher than those in model group (P<0.05), and no significant change was observed between captopril group and model group. CONCLUSION: Xinkang recipe therapy may improve cardiac function in heart failure rats, which may be related to inhibiting the expression of miR-25-3p, increasing the SERCA2a/PLB ratio and enhancing SERCA2a activity in the myocardium.     

中兽医治疗奶牛腹泻良方与临床应用

腹泻是以排便次数增多、便稀溏为特征的一类疾病,根据不同特点又可分为泄泻、痢疾、便血三大类,每一类叉分为不同的证型。以临床病例为例,阐述腹泻的辨证方法、有效良方的选择及加减应用,既有同病同治、异病异治的常规方法,也有同病异治、异病同治的变化方法。     

中药复方制剂抗猪流感病毒作用的研究

[目的]探讨中药复方制剂(金银花、连翘、薄荷、牛蒡子、桔梗、甘草、淡竹叶、淡豆豉)对感染猪流感病毒小鼠的保护作用。[方法]猪流感病毒感染小鼠4 h后给药,以死亡率、平均存活时间、肺指数、肺指数抑制率、血凝滴度、脾脏指数、胸腺指数为评价指标,观察中药复方制剂在小鼠体内的抗流感病毒作用。[结果]中药复方制剂能降低流感病毒感染小鼠的死亡率(P0.01),延长小鼠的存活时间(P0.01),降低肺指数(P0.01),降低血凝滴度(P0.01),增加胸腺指数(P0.01)。[结论]该中药复方制剂具有良好的抗猪流感病毒作用。     

低质早籼米配米配方及其品质的研究

为改善低质早籼米品质，采取配米技术，对基米与其配米米质进行比较分析，试验材料包括19个水稻品种，即5个低质早籼品种、9个晚籼品种、3个糯稻品种和2个粳稻品种，结果表明，配米技术能明显改善早籼米品质，且其配方比例具有相似性，3种基米的早籼配米配方比例为低质早籼米66.41%，优质米16.87%，糯稻米16.72%，4种基米的早籼配米配方比例为低质早籼米62.39%，优质米12.68%，糯稻米12.67％,粳稻米14.31%，最好的配米组合为湘早籼26号（62.837%），湘晚籼10号（12.187%），晚糯91-581（12.671%），晚粳94046（12.304%）。