长期施肥条件下土壤养分的动态和平衡——Ⅱ.对土壤氮的有效性和腐殖质氮组成的影响

长期施用有机肥、化肥或二者配合施用均增加了土壤全氮、碱解氮、酸解氮和植物吸收氮量,提高了土壤氮的有效性,其后效可保持至第3年;土壤腐殖质的胡敏酸氮减少,富里酸氮增加。富里酸氮易被6mol/L HCl水解,水解氮占71.4％～74.2％;胡敏酸氮比较稳定,水解氮占40％～45％。在水解氮中,氨基氮的比例为2/3～3/4,铵态氮为1/4～1/3。胡敏酸中非水解氦的主要成分是杂环化合物氮(37.5％～52.5％),其次是苯系化合物氮(31％～35.2％)和非苯系化合物氮(10.6％～26.7％)。长期施用有机肥和化肥,杂环氮比例减少,苯系和非苯系化合物氮比例增加。胡敏酸表层氮占1/3左右,核心氮占2/3左右,施用有机肥和化肥,胡敏酸表层氮增加,核心氮降低。     

我国陆生生态化学计量学应用研究进展与展望

生态化学计量学是一门集生态学、化学计量学、物理学的交叉学科,是当前生态学研究的热点和前沿领域,为研究植物限制性元素及生态系统物质循环提供了一种新的方法和思路。本文重点从气候变化与氮沉降、生态系统演化、添加实验及人类活动等对生态化学计量特征的影响方面对我国陆生生态化学计量学的应用研究进行了总结与评述,并结合现已开展的工作,对有待进一步拓展的相关生态化学计量研究领域进行了展望,以期在生态系统稳定性、常量元素调控等方面的研究取得新突破。     

土壤微生物体氮与可矿化氮关系的研究

同时应用大田试验和室内培养试验研究土壤微生物体氮与可矿化氮之间的相关性。试验结果表明,田间条件下,土壤微生物体氮与可矿化氮之间的关系不密切,但在培养试验中,微生物体氮与淹水培养法、硝化培养法和Stanford短期淋洗通气法测定的可矿化氮间有显著的线性关系,相关系数在0.767(p<0.01,n=12)以上。田间试验结果和室内培养试验结果的不一致性,与试验条件的差异有关。新形成的微生物体氮易降解,而原有土壤微生物体氮却相对稳定。     

氮肥用量及施用时间对土体中硝态氮移动的影响

土连续两年小麦—玉米轮作条件下 ,播前一次施氮量 130～ 5 2 0kghm-2 a-1时 ,氮肥用量对硝态氮在土体中的移动深度没有影响 ,但土壤剖面中残留的硝态氮量随施氮量增加显著增加。播前一次施用氮肥 ,差减法计算的肥料氮表观回收率 (作物携出量和土壤硝态氮的残留量 )为 6 2 %～ 82 7% ;就作物而言小麦的携出率高于玉米 ,在玉米生长季节有更多的硝态氮可能被淋移至土壤剖面的下层。小麦—玉米轮作一年 ,不同的施氮时间对肥料氮的表观回收率以及硝态氮在土壤剖面中的分布、累计没有明显影响。土区合适的氮肥用量是控制硝态氮向深层移动的主要措施     

土壤微生物体氮的季节性变化及其与土壤水分和温度的关系

以杨陵土垫旱耕人为土(中等肥力红油土)为供试土壤进行田间试验和室内培养试验,研究土壤微生物体氮的动态变化及其土壤含水量和温度的关系。结果表明,田间土壤微生物体氮的变化有明显的季节性;夏季最高,冬季最低,其它时期居中;且与土壤温度有显著或极显著的正相关性,相关系数在0.855以上;试验期间土壤水分含量在10%以上,基本能满足微生物活动所需,因而微生物体氮的变化与水分关系并不密切。应用培养试验结果进一步证明了田间试验结果,即在4～36℃范围内,微生物体氮与温度呈线性相关,而在土壤含水量为6.75%～23.23%范围内,与水分呈指数相关关系,当土壤水分小于10.87%时,水分对微生物体氮有突出结果,当超过10.87%后,几乎没有影响。频繁的干湿交替会使微生物体氮显著减少,但冻融交替却无明显影响。     

灌溉方法对保护地土壤有机氮组分及剖面分布的影响

用Bremner有机氮分组法测定了连续7年采用不同灌溉方法灌溉的保护地土壤各剖面层次有机氮组分含量。结果表明,土壤全氮及有机氮各组分含量均随土层深度的增加而降低,但有机氮各组分占全氮的比例随土层深度增加的变化却无明显规律。用3种灌溉方法灌溉,不同土层间土壤有机氮各组分含量的差异主要存在于0～50 cm土层,50 cm以下差异很小;相同土层,土壤有机态氮含量均以酸解氮为主,且酸解氮中各组分绝对含量和相对含量的大小排列顺序均为,未知态氮〉氨态氮〉氨基酸态氮〉氨基糖态氮。在0～80 cm土层,土壤酸解氮占全氮的比例大多在58%～60%之间,只有渗灌处理0～10 cm,10～20 cm及沟灌处理0～10 cm土层酸解氮占全氮的比例较低,分别为34.21%,50.75%和48.02%;而非酸解氮占全氮的比例大多在32%～36%之间。3种灌溉方法相比较,除个别层次外,酸解氮中氨基酸态氮、氨基糖态氮及氨态氮占全氮的比例在各土层中滴灌和渗灌处理均高于沟灌处理,而酸解未知态氮和非酸解氮占全氮的比例则为沟灌处理高于滴灌和渗灌处理。     

中国土壤氮含量、空间格局及其环境控制

Soil holds the largest nitrogen （N） pool in terrestrial ecosystems, but estimates of soil N stock remain controversial. Storage and spatial distribution of soil N in China were estimated and the relationships between soil N density and environmental factors were explored using data from China＇s Second National Soil Survey and field investigation in northwest China and the Tibetan Plateau. China＇s soil N storage at a depth of one meter was estimated at 7.4 Pg, with an average density of 0.84 kg m^-2. Soil N density appeared to be high in southwest and northeast China and low in the middle areas of the country. Soil N density increased from the arid to semi-arid zone in northern China, and decreased from cold-temperate to tropical zone in the eastern part of the country. An analysis of general linear model suggested that climate and vegetation determined the spatial pattern of soil N density for natural vegetation, which explained 75.4% of the total variance.     

稻田土壤上控释氮肥的氮素利用率与硝态氮的淋溶损失

在稻田土壤上对水稻的高量施用氮肥常常造成硝态氮(NO3--N)淋溶损失和肥料氮利用率低下的问题。本研究采用土壤渗漏器、微区和田间小区试验,研究了15N标记控释氮肥在稻田土壤上的氮素利用率和硝态氮的淋溶损失。在两年早稻种植期间,一次性全量作基肥施用控释氮肥与尿素分二次施用的相比,两年的早稻产量分别平均提高7.7%和11.6%。在N90 kg hm-2用量下,由差值法测得的肥料氮利用率,按平均计,控释氮肥的N利用率(平均76.3%)比尿素分次施用的(平均37.4%)高出38.9%1。5N同位素法测得的控释氮肥的N利用率(平均67.1%)比尿素分次施用的(平均31.2%)高出35.9%。在早稻种植季节,施用尿素和控释氮肥的NO3--N淋失量分别为9.19 kg hm-2和6.7 kg hm-2,占施尿素N和控释氮肥氮的10.2%和7.4%。控释氮肥的氮淋失量比尿素分2次施用的降低27.1%。本研究结果表明,在稻田土壤上施用控释氮肥能减少氮的淋失量,提高氮素利用率和水稻产量。     

Nutrient Uptake and Use Efficiency of Irrigated Rice in Response to Potassium Application

Potassium is one of the most important nutrients for rice production in many areas of Asia, especially in southeast China where potassium deficiency in soil is a widespread problem. Field experiments were conducted for four consecutive years in Jinhua City, Zhejiang Province, to determine utilization of nutrients (N,P and K) by inbred and hybrid rice and rice grain yields as affected by application of potassium fertilizer under irrigated conditions. Grain yield and nutrient harvest index showed a significant response to the NPK treatment as compared to the NP treatment. This suggested that potassium improved transfer of nitrogen and phosphorus from stems and leaves to panicles in rice plants. N and P use efficiencies of rice were not strongly responsive to potassium, but K use efficiency decreased significantly despite the fact that the amount of total K uptake increased. A significant difference between varieties was also observed with respect to nutrient uptake and use efficiency. Hybrid rice exhibited physiological advantage in N and P uptake and use efficiency over inbred rice. Analysis of annual dynamic change of exchangeable K and non-exchangeable K in the test soil indicated that non-exchangeable K was an important K source for rice. Potassium application caused an annual decrease in the concentration of available K in the soil tested, whereas an increase was observed in non-exchangeable K. It could be concluded that K fertilizer application at the rate of 100 kg ha^-1 per season was not high enough to match K output, and efficient K management for rice must be based on the K input/output balance.     

洞庭湖区不同土地利用方式耕作土壤氮素含量与循环

通过对洞庭湖典型地区的密集采样分析和农户调查,研究了4种利用方式耕作土壤全N、微生物生物量氮(MB-N)含量、两者关系和N素循环特征。结果表明:耕作土壤全N、MB-N含量平均值为3.00±0.48g/kg和101.4±49.2mg/kg。双季稻、一季稻、水田旱作和旱地全N平均含量依次为3.12±0.40g/kg、3.03±0.39g/kg、2.79±0.43g/kg2、.10±0.46g/kg。4种利用方式的MB-N含量分别为124.0±56.6mg/kg、96.4±39.2mg/kg、108.0±48.6mg/kg、75.2±30.5mg/kg。除水田旱作外,MB-N与全N之间存在极显著的正相关关系(P<0.01)。土壤N素盈余量依次为双季稻(105.0kg/hm2.a)>一季稻(75.1kg/hm2.a)>水田旱作油菜(64.5kg/hm2.a)>旱地苎麻(51.9kg/hm2.a)。