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宠物犬猫的难产,在临床上是一种原因较复杂也较常见的产科疾病。笔者自1993-1998年间,共计收治35例,其中剖腹手术治疗的共计29例,成功率为100%。现将治疗过程介绍如下: 相似文献
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主动外排系统介导的大肠杆菌多重耐药性的确证 总被引:9,自引:1,他引:8
为检测和证实主动外排系统(外输泵)在细菌多重耐药机制中的作用,用四环素和水扬酸钠对大肠杆菌质控株进行了体外人工诱导,筛选出多重耐药株,再用能量抑制剂CCCP(Carbongl cyanide m-chloropheyhydrazone)时耐药株和质控株进行了环丙沙星摄取抑制试验。结果:在耐药株,其菌体内环丙沙星稳态浓度明显低于质控株;多重耐药株在有CCCP存在时,菌体内环丙沙星的浓度随时间的延长而递增,而无CCCP的抑制时,菌体内环丙沙星的浓度递增缓慢;在质控株,不论有无CCCP存在,菌体内环丙沙星的浓度都随时间的延长而呈递增趋势,且保持较耐药株明显高的水平。结论:体外诱导多重耐药大肠杆菌多重耐药性的产生,可能主要依赖于菌体外输泵的激活。 相似文献
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抗鸡致病性大肠杆菌中药的筛选及其疗效观察 总被引:3,自引:0,他引:3
[目的]为获得有效防治鸡大肠杆菌病的中药制剂奠定基础。[方法]以西药盐酸洛美沙星氯化钠注射液为对照,对15味中药及7个自拟中药复方进行药敏试验,并对7个中药复方和体外抑菌效果较好的单味中药进行体内试验。[结果]药敏试验结果表明,15味单味中药均有一定的抗大肠杆菌作用,其中以芦荟、石榴皮、地榆等抑菌效果明显。7个自拟复方中复方一、复方二和复方五的抗大肠杆菌效果较好。体内试验结果表明,中药对人工感染大肠杆菌的鸡只治疗效果优于西药,以复方一和复方二效果较好。[结论]中药制剂对鸡大肠杆菌病具有较好的治疗效果,但其在体内与体外的抑菌作用机制有所不同。 相似文献
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[Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [Method] With the western medicine Lomefloxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional Chinese herbs and seven self-made Chinese herbal compounds were conducted. Subsequently, the single traditional Chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E.coli to observe their efficacies. [Result] The results of drug sensitivity tests showed that all the 15 traditional Chinese herbs had certain antibacterial effect against E.coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional Chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [Conclusion] Traditional Chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro. 相似文献
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对Ⅰ型马立克氏病毒的pp38基N进行了扩增、克隆及序列测定。采用DNAStar Protean软件对pp38蛋白的二级结构、亲水性和抗原性指数等参数进行分析,并预测其B细胞表位分布。结果表明,pp38基N长873bp,包含完整的开放阅读框架,编码290个氨基酸。蛋白的B细胞表位可能位于第1~11,18~162,180-217和274-290位等氨基酸区域内或附近。本试验为pp38蛋白的表达和抗体制备奠定了理论基础。 相似文献