猪繁殖与呼吸综合征的概述

正猪繁殖与呼吸综合征(PRRS)首次报道是1987年在美国。从那时起,PRRS疫情和病毒的成功分离已在整个北美和欧洲被发现或证实。1病因及流行病学病原体是组动脉炎的病毒。该病毒有包膜,个体大小从45至80 mm。病毒经乙醚或氯仿处理后可能会灭活;然而,该病毒在冷冻条件下极其稳定,在-70℃下其感染性能保持4个月,随着温度的升高,感染性降低(56℃下15~20 min)。     

青贮玉米新品种筛选试验

通过对不同来源的19个青贮玉米杂交种的生育期、生物性状和经济性状的筛选及评价，结果表明，SD3玉米品种可以在昭苏地区推广种植。     

广西农机化百年大事记

正(接2013年第5期)2005年1月6日自治区农机化管理中心对南宁市等14个水稻跨区机收先进单位、杨德恩等16名跨区机收先进工作者和卢顺球等20名跨区机收作业能手予以表彰。2月全国跨区机收工作领导小组授予玉林市、资源县全国     

诚实信用原则及适用之我见

诚实信用原则是对民事主体的民事行为和经济活动所设定的道德底线,它首先是一个道德准则,经过法律技术化后成为民法的基本原则。当前我国实行的是社会主义市场经济体制,市场经济有效运行的基础,一方面是法律,一方面是道德,而诚实信用原则正是两者结合的典范。诚实信用原则在民事活动中的重要性虽然已经得到我国立法的肯定,但在我国当前的市场经济运行中并未受到应有的尊重,诚信的缺失已成为制约我国经济发展的障碍。笔者拟从有利于市场经济发展的目的出发,对诚实信用原则的内涵,地位、内容、适用、违反及其后果等方面做一详致的分析。     

在拼读中开始快乐的交流——内初班拼音教学初探

内初班学生在汉语文学习上存在的较大困难,就是听不懂,也不开口.学生在内初班伊始,就是学习汉语拼音.掌握好这根识字说话的“拐杖”,就为能说好普通话,学好汉语言夯实了基础.     

土壤化学分析方法(一)

1980年新西兰专家D. W. 艾夫斯来我省并赠送省农业厅土肥处一本“土壤化学分析方法”(A Method for chemical analysisof softs)。该书所介绍的方法是新西兰科学技术研究部土壤局长期以来采用并发展起来的方法。该书对方法原理介绍得极为简     

番茄叶霉病菌拮抗链霉菌BPS2发酵条件的研究

(1吉林省农业科学院，公主岭 136100；2东北农业大学资源与环境学院，哈尔滨 150030； 3吉林农业大学农学院，长春 130118)     

Improving Drought Tolerance of Rice by Designed QTL Pyramiding

Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem, we developed a new strategy ＇designed QTL pyramiding＇ to more efficiently develop drought tolerant （DT） rice cultivars. In the first phase of this strategy, we developed large numbers of introgression lines （ILs） in elite backgrounds using backcross （BC） breeding, each of which carries multiple genomic segments for improved DT from a known donor. Then, we genotyped all ILs with SSR markers to track the genomewide pattern of introgression in the DT ILs of 16 BC populations derived from crosses between 2 recipients （IR64 and Teqing） and four different donors. X^2 tests and linkage disequilibrium analyses of introgression in the DT ILs revealed significant frequency shifts at many loci across the genome and non-random associations at the multilocus level as a result of selection for DT in the ILs, which led us to the discovery of putative genetic networks underlying DT in the ILs. The networks each containing all DT loci detected in ILs from a BC population showed some interesting features.     

TILLING and Ecotilling the Plant Kingdom

The modem crop scientist has a large amount of available nucleotide sequence information to identify genes of potential agronomic importance. Using reverse genetic approaches, specific genes can be disrupted, and hypotheses regarding gene function directly tested in vivo. Although a number of reverse genetic methods have been introduced, many are limited in application because they are organism-specific, expensive, transgenic, or only transiently disrupt gene function. However, traditional mutagenesis using chemical mutagens has been widely used as a forward genetics strategy to create many new crop plant varieties at relatively low cost. Mutagens such as ethyl methanesulphonate （EMS） cause stable point mutations and thus produce an allelic series of truncation and missense changes that can provide a range of phenotypes （Greene et al., 2003）. TILLING （targeting induced local lesions IN genomes） is a high-throughput reverse genetic strategy that combines traditional mutagenesis and SNP discovery methods （Colbert et al., 2001; McCallum et al., 2000）. To identify mutations, target regions of-l.5 kb are amplified with fluorescently labeled gene specific primers. Heteroduplexes are then formed between wild-type and mutant strands, mismatches are cleaved by incubation with a single-strand specific nuclease,