Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies

Microbial digestive enzymes in soil and litter have been studied for over a half century, yet the understanding of microbial enzymes as drivers of ecosystem processes remains hindered by methodological differences among researchers and laboratories. Modern techniques enable the comparison of enzyme activities from different sites and experiments, but most researchers do not optimize enzyme assay methods for their study sites, and thus may not properly assay potential enzyme activity. In this review, we characterize important procedural details of enzyme assays, and define the steps necessary to properly assay potential enzyme activities in environmental samples. We make the following recommendations to investigators measuring soil enzyme activities: 1) run enzyme assays at the environmental pH and temperature; 2) run proper standards, and if using fluorescent substrates with NaOH addition, use a standard time of 1 min between the addition of NaOH and reading in a fluorometer; 3) run enzyme assays under saturating substrate concentrations to ensure V_max is being measured; 4) confirm that product is produced linearly over the duration of the assay; 5) examine whether mixing during the reaction is necessary to properly measure enzyme activity; 6) find the balance between dilution of soil homogenate and assay variation; and 7) ensure that enzyme activity values are properly calculated. These steps should help develop a unified understanding of enzyme activities in ecosystem ecology.     

肥液浓度对单膜孔入渗NO-3-N运移特性影响的室内试验研究

该文通过室内入渗试验，研究了不同浓度的单膜孔肥液入渗NO^-₃-N的分布特性。研究表明：不同浓度的膜孔肥液入渗土壤NO^-₃-N浓度的湿润锋运移距离与土壤水分运动的湿润锋一致；肥液浓度越大，相同入渗时间的NO^-₃-N浓度锋运移距离越大，土壤剖面NO^-₃-N浓度最大值越大，相同深度处土壤NO^-₃-N浓度也越大。肥液入渗土壤NO^-₃-N浓度分布特征与湿润体深度符合分段函数模型。供水入渗过程中，NO^-₃-N浓度锋运移距离和浓度最大值均随时间的延长而增大；再分布过程中，NO^-₃-N浓度锋运移距离继续增大，而NO^-₃-N浓度最大值逐渐减小。     

Foliar application of iron,zinc, and manganese nano-chelates improves physiological indicators and soybean yield under water deficit stress

Abstract

To investigate the effect of foliar application of nano-chelates of iron, zinc, and manganese subjected to different irrigation conditions on physiological traits, and yield of soybean (cultivar M9), a split plot experiment was conducted in a completely randomized block design with three replications in two crop years (2016–2017). The main plot included four levels of irrigation (I): full irrigation (I ₁), irrigation withhold at flowering stage (I ₂), irrigation withhold at podding stage (I ₃), and irrigation withhold during the grain filling period (I ₄). Also, the subplot included eight levels of foliar application with Fe, Zn, Mn, Fe?+?Zn, Fe?+?Mn, Zn?+?Mn, Fe?+?Zn?+?Mn nano-chelates, and distilled water (control). The results of combined analysis of variance suggested that the effect of irrigation and foliar application of nano-chelate was significant on all traits. Water deficit stress significantly reduced the grain yield. The minimum numbers of pods per plant, number of grains per plant, 100-seed weight per plant, leaf area index, leaf chlorophyll concentration, total dry weight of plant, and the grain yield were obtained by irrigation withhold at podding stage. Foliar application of combined nano-chelates increased the soybean resistance against water shortage more considerably than the separate consumption of these elements. Under drought stress in podding stage, the application of Fe?+?Zn led to the highest yield with a mean of 2613.84?kg ha^?1 where this increase was 61.1% higher than control.     

大豆幼苗光合特性对锰营养的响应

采用溶液培养方法,设Mn2+浓度为0、0.05、0.50、5、30、50.mg/L,探讨了2种大豆品种(浙春2号、东北大豆854-11)的幼苗光合特性对不同锰浓度的响应。结果表明,低锰浓度提高了大豆叶片的初始荧光(Fo)、最大荧光(Fm)、PSⅡ原初光能转化效率(Fv/Fm)、潜在光化学活性(Fv/Fo)和光化学猝灭系数(qP),高锰降低了Fo、Fm、Fv/Fm、Fv/Fo、qP。随着锰营养的增加,非光化学猝灭系数(qN)增大。适量的锰浓度显著提高了大豆的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs),降低了气孔阻力(Rs)和细胞间CO2浓度(Ci),随着锰浓度的逐渐增大,降低了Pn、Tr、Gs,提高了Rs、Ci。0.50.mg/L下的锰浓度有最大的Fo,5.mg/L下的锰浓度有最大的Fm、qP、Fv/Fm、Fv/Fo,表明0.505～mg/L的锰浓度有利于大豆的光合作用。在50.mg/L的锰浓度下,两个大豆品种有最大的qN、Rs、Ci和最小的Tr、Pn,此时两个品种大豆耗散了过剩的激发能,降低了大豆叶片的光合速率,对大豆已产生了一定的伤害。两个品种大豆光合特性对锰的响应存在着基因型差异,浙春2号较东北大豆耐锰胁迫。     

密闭空间药雾浓度测量的试验方法研究

运用改进后的大气采样器，建立了一种对密闭空间细雾流分批采样分批分析的研究方法。对于采样参数组合的选取以及双侧采样的空间差异性进行了研究和统计判断，得出了最佳采样参数组合为采样时间5min，采气量5L／min。基于最佳采样参数组合，发现沉降曲线中存在分段点现象，分段点在喷雾结束后13min，也即开始喷雾后27min附近。针对分段点现象，给出了分段拟合的细雾流浓度沉降曲线及解析描述。采用改进的大气采样器可以获取喷雾结束后79rnin内的浓度数据，文献[13]中所用的采样方法只能得到喷雾结束后45min左右的浓度数据，因此该方法对药雾浓度沉降曲线的尾部特征描述能提供更多的信息。     

沼液负压蒸发浓缩装置的设计与试验

为了解决沼液浓缩过程中有效成分流失的问题,采用低温负压蒸发浓缩的方法,结合沼液的理化性质,对沼液负压蒸发浓缩装置的主体部分(蒸发罐)和辅助设备(冷凝器、循环液槽、真空泵)分别进行了参数设计计算和设备选型,并在自制20 L负压蒸发浓缩装置上进行了系列试验。试验结果表明:当温度低于80℃时,沼液的有机质含量基本不变,蒸发温度高于80℃之后,有机质含量明显下降,80~100℃的变化率为9.4%;随着温度的升高,氮磷钾含量不断下降,氮含量流失最大,最大变化率为13%。蒸发浓缩后的沼液中有机质和氮磷钾含量随真空度的增大而升高;随着真空度的升高,沼液的蒸发温度逐渐降低,蒸发量不断增大,绝对真空度为0.025 MPa时,最低蒸发温度为67℃,蒸发量最高能达到8 324 m L/(m2·h),蒸发率为28.75%;绝对真空度维持在0.04 MPa到0.05 MPa之间,温度在75~80℃时,蒸发量能达到7 700 m L/(m2·h),蒸发率为26%左右。     

黄土高原坡耕地泥沙输移特征分析

在野外利用人工模拟降雨试验,分析了20,40,60m坡长下的输沙率、含沙量及累积产沙量—径流量的变化特征。结果显示,当雨强小于2.0mm/min时,最大输沙率出现在40m坡长;而当雨强大于2.0mm/min时,最大输沙率出现在60m坡长。在试验雨强范围内,最大含沙量总是出现在20m坡长。累积产沙量—径流量随时间变化趋于平稳。试验结果表明,为遏制黄土高原坡耕地水土流失,实践中应采取垄作等措施增加坡面糙度和改变坡面形态,防止在20m坡长形成高含沙水流和后续坡长的输沙率。     

覆膜旱作稻N、P、K养分利用特征

在田间试验条件下对覆膜旱作稻体内N、P、K养分浓度、吸收动态及N肥利用率作了研究。结果表明,相同施肥处理条件下不同生育期覆膜旱作稻N、P、K养分浓度及吸收量均高于水作稻,尤以生育中后期较为明显;覆膜旱作稻全生育期N、P、K养分吸收量明显高于水作稻,分布在籽粒中的N、P、K养分含量也有所提高,而在整株中的占有率差异不大,N肥利用率提高12%左右。     

高钙饲粮对青年蛋鸡血清一氧化氮浓度和抗氧化功能的影响

本试验旨在研究高钙饲粮对青年蛋鸡血清一氧化氮（ＮＯ）浓度和抗氧化功能的影响。将 ３５日龄 １００羽伊沙蛋鸡随机均分为对照组（含钙 １．００％）和高钙组（含钙 ３．７８％），每组 ５个重复，每个重复 １０羽。每天观察鸡的精神状态、食欲和粪便情况。在饲喂高钙饲粮第 ０、８、１６、２４和 ３２天时，分别测定 ２组鸡的血清中 ＮＯ浓度和丙二醛含量及抗氧化酶系统的变化。结果表明：１）高钙饲粮能引起青年蛋鸡长期腹泻，在饲喂高钙饲粮的第 ８天就发生高血钙症。２）与对照组相比，高钙组在饲喂高钙饲粮第 ８天和第 １６天血清 ＮＯ浓度无显著变化（Ｐ＞０．０５），第２４天和第 ３２天显著升高（Ｐ＜０．０５）；第 ８天和第 ２４天血清超氧化物歧化酶活性、总抗氧化能力无显著变化（Ｐ＞０．０５），第 １６天显著升高（Ｐ＜０．０５），第 ３２天显著降低（Ｐ＜０．０５）；第 ８、１６、２４天血清丙二醛含量无显著变化（Ｐ＞０．０５），第 ３２天显著升高（Ｐ＜０．０５）；第 ２４天和第 ３２天黄嘌呤氧化酶活性有升高趋势（Ｐ＞０．０５）。结果提示，饲喂高钙饲粮可导致青年蛋鸡发生长期腹泻，引起高血钙症，导致其机体内自由基的产生与清除的动态平衡遭到破坏，使自由基的产生远远超出清除能力。