剪叶对槟榔生长状况的影响

为探寻促使槟榔(Areca catechu)叶片早脱落,槟榔提前开花的方法,以七年生海南本地槟榔为试验对象,对槟榔最下面3片大叶进行纵向不同程度剪叶,研究其对叶片含水量、叶绿素含量及内含物的影响,以此了解剪叶对槟榔生长状况的影响.结果表明,剪叶处理对槟榔生长状况产生了一定的影响,剪叶程度不同产生的影响也不同.对照组中即自然状态下叶片含水量一直处于下降趋势,纵向剪叶可使叶片含水量处于比较平稳的状态;剪叶对最下面的叶片叶绿素含量影响明显,剪叶程度越大越明显,但对于整体叶绿素含量无明显影响;剪叶后蛋白质含量先升后降;淀粉含量先降再升再降再升;可溶性糖含量先升高后降低最后保持平衡状态.     

稻瘟病胁迫下水稻叶片叶绿素含量与光谱特征参数的相关性研究

为实现受稻瘟病侵染水稻叶片叶绿素含量的高光谱反演,以‘陵两优268’为研究对象,测定受稻瘟病侵染的85个水稻叶片样品的叶绿素含量和高光谱反射率,分析受稻瘟病侵染的水稻叶片高光谱反射率与叶绿素含量间的相关关系,使用线性与非线性回归技术建立叶绿素含量反演模型。结果显示:叶绿素含量与原始光谱及一阶导数光谱的敏感波段分别发生在700 nm和752 nm,基于光谱特征参数SDr的回归模型均方根误差为1.27,平均相对误差为10.2%。研究表明受稻瘟病侵染水稻叶片光谱反射率差异明显,基于光谱特征参数SDr的回归模型预测叶绿素含量具有较高的精度。     

基于计算机视觉技术的玉米叶绿素含量检测研究

植株叶片中叶绿素浓度的高低与植株进行的光合作用效率、植株的整体生长状况息息相关,在农业生产过程中,常常根据叶片中叶绿素含量(SPAD)的多少来精确的判断植物的生长状态,也是控制植株长势的依据。传统的叶绿素含量检测方式分光光度法,存在耗时长、步骤多、操作要求高等问题,而采用计算机视觉技术处理图像的过程更加准确、高效,不会像人眼分析时受到主观因素的影响导致偏差。为此,基于计算机视觉技术来检测玉米叶片中叶绿素含量,利用扫描仪采集玉米叶片的图像,将图像输送至计算机,然后通过软件处理图像,分割出图像中有效像素的颜色特征值,将特征值转换就可以得到玉米叶片中叶绿素。试验结果显示:利用计算机视觉技术可以准确地测定玉米叶片中叶绿素含量,进而进行合理施肥,避免浪费,对增加玉米的产量具有极大的价值。     

Postharvest UV-C treatment combined with 1-methylcyclopropene (1-MCP), followed by storage in continuous low-level ethylene atmosphere,improves the quality of tomatoes

Mature green tomatoes (Solanum lycopersicum L. cv Neang Pich) were exposed to 13.6 kJ m^?2 UV-C or 0.5 μL L^?1 1-MCP or combination of 13.6 kJ m^?2 UV-C and 0.5 μL L^?1 1-MCP, with appropriate untreated controls. After treatment, tomatoes were stored in air containing 0.1 μL L^?1 ethylene at 20°C and 100% RH. The untreated fruit ripened significantly faster than those of all other treatments. UV-C treatment alone was able to delay fruit ripening by up to 5 days longer compared to untreated fruits whilst the additional of 1-MCP further delayed fruit ripening. UV-C and 1-MCP treatments alone or in combination had significantly slower ethylene production rates throughout the storage period. The fruit treated with the combination of 1-MCP and UV-C was significantly firmer and had higher total phenolic content compared to that of the other treatments. However, there was no difference between treatments in soluble solids content/titratable acids ratio, chlorophyll content, lycopene content and total antioxidant activity. These results show that UV-C and 1-MCP treatment delay ripening and improve the quality of tomatoes in the presence of low-level ethylene during storage. This new treatment could be used to extend the shelf-life of mature green tomatoes through the supply chain without the use of refrigeration.     

Potassium deficiency impedes elevated carbon dioxide‐induced biomass enhancement in well watered or drought‐stressed bread wheat

Potassium (K) deficiency reduces photosynthesis and biomass production of crop plants and also renders them vulnerable to drought stress, whereas elevated carbon dioxide (CO₂) has a positive effect on photosynthesis and yield and ameliorates the adverse effects of drought stress. This study aimed to characterize the physiological responses of wheat (Triticum aestivum L.) stressed with K deficiency under elevated CO₂ and drought conditions. Increased biomass production caused by elevated CO₂ as a consequence of increased photosynthesis and water use efficiency was absent in young K‐deficient wheat plants. Shoot K concentration was negatively affected by elevated CO₂ particularly under K‐deficient conditions, whereas K content per plant was greatest in plants supplied with adequate K and adequate water. Specific leaf weight was increased as a consequence of carbohydrate accumulation in the source leaves of K‐deficient plants particularly under elevated CO₂ and drought stress. Potassium deficiency clearly impeded the impact of elevated CO₂ in both well watered as well as drought‐stressed plants. Adequate K fertilization is a prerequisite for efficient harvesting of atmospheric CO₂ through increased photosynthesis, decreased transpiration, and increased biomass production under changing atmospheric CO₂ and soil moisture conditions.     

Effects of 5‐aminolevulinic acid on water uptake,ionic toxicity,and antioxidant capacity of Swiss chard (Beta vulgaris L.) under sodic‐alkaline conditions

Sodic‐alkalinity may be more deleterious to plant growth than salinity. The objectives of this study were to determine whether 5‐aminolevulinic acid (ALA: an essential precursor for chlorophyll biosynthesis) foliar application could improve the sodic‐alkaline resistance of Swiss chard (Beta vulgaris L. subsp. cicla ) by regulating water uptake, ionic homeostasis, photosynthetic capacity, and antioxidant metabolism. Eight‐week‐old uniform plants were grown in nutrient medium without and with a sodic‐alkaline regime generated by a mixture of NaHCO₃ and Na₂CO₃ (NaHCO₃ : Na₂CO₃ = 9:1 molar ratio) for 12 d, and leaves were sprayed daily with water or ALA. The Na⁺ and ALA concentrations were gradually increased to 60 mM and 120 μM, respectively. ALA foliar application alleviated the physiological damage from sodic‐alkalinity, as reflected by the increases in plant dry weight, relative growth rate, chlorophyll, Mg²⁺ concentration, and the decrease in Na⁺ concentration. However, ALA foliar application did not change the water uptake capacity or the concentration of K⁺, Fe³⁺, and endogenous ALA in leaf tissues under sodic‐alkaline conditions. ALA foliar application effectively mitigated damage from sodic‐alkalinity because of the increased activity of antioxidant enzymes (catalase and guaiacol peroxidase), particularly superoxide dismutase activity, which was maintained at the same level as for control plants. These results suggest that ALA foliar application alleviated sodic‐alkaline stress mainly owing to its antioxidant capacity, and superoxide dismutase has the main responsibility for reducing oxidative stress in Swiss chard.     

覆盖对烟田土壤酶活性及烤烟叶片荧光特性的影响

通过对烤烟大田3种不同覆盖方式的覆盖效应比较表明:覆盖明显增强了土壤过氧化氢酶和蔗糖酶活性,对脲酶活性有一定抑制作用;在烤烟生长前期,液膜覆盖处理土壤过氧化氢酶活性较对照和地膜覆盖处理分别平均提高5.72%、23.19%,且覆盖处理间差异达0.05显著水平;覆盖处理的蔗糖酶较对照平均提高0.8%,但3个处理间差异均不显...     

施肥对黑麦草生长和产量的影响

通过大田试验,研究了不同施肥措施对一年生黑麦草(Lolium multiflorum)生长、叶绿素含量和鲜草产量的影响.结果表明,氮磷钾肥配合施用(NPK)、等氮量的有机肥(OM)处理均显著促进黑麦草的生长和提高其鲜草产量.NPK处理的黑麦草分蘖数、株高、表观叶面积较不施肥对照(CK)、不施氮(PK)和不施磷(NK)处...     

Effect of hot air,UV-C,white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets

Several treatments were applied in order to delay postharvest degreening in broccoli florets and investigate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.     

盐胁迫和干旱胁迫对蓝花子种子萌发和幼苗生长的影响

将蓝花子培养在含有不同浓度的NaCl和甘露醇的MS培养基上,研究蓝花子在不同浓度的NaCl和甘露醇处理下的发芽势、发芽率、相对发芽率、叶绿素含量以及过氧化物酶活性等,揭示蓝花子对盐胁迫和干旱胁迫的耐受能力。结果表明:蓝花子的发芽率、株高、根长、叶绿素以及过氧化物酶活性都随NaCl浓度的升高而显著下降;在甘露醇胁迫时,蓝花子幼苗的根长、叶绿素含量以及POD酶活性都随甘露醇浓度的升高表现为先升高后降低的趋势。说明同十字花科其它植物相比蓝花子对盐胁迫十分敏感,而对干旱胁迫却能表现出比较明显的耐受性。