鹿茸多肽提取分离纯化及药理作用研究进展

鹿茸多肽具有抗炎、抗氧化、增强免疫并能促进细胞分化、伤口愈合、抑制肿瘤细胞、促进神经系统、周围神经系统组织的再生等药理作用,是鹿茸中最有效的成分之一。文中对鹿茸多肽的提取,分离纯化及药理作用研究进展进行了综述,希望为鹿茸多肽的研究和开发提供参考。     

吉林省民营高等学校资源配置效率研究

民营高等学校是我国重要的人才培养渠道,自20世纪80年代以来,民营高等教育进入了飞速发展时期。吉林省民营高校经过多年的改革和探索已有了跨越式的发展,但如何使其从粗放型发展向内涵式发展转变,实现教育资源合理配置是关键。本文从调查研究吉林省民营高等学校资源配置的实际情况出发,采用数据包络分析方法（DEA）,评价民营高等学校资源配置效率问题,可以看出,吉林省民营高等院校在资源配置上依然存在着非效率的情况,本文据此提出相应建议。     

木耳属4个野生菌株的培养特性研究

通过研究不同营养和环境条件对木耳属4个主要种类的野生菌株生长速率和生长势等培养特性的影响,明确了黑木耳、毛木耳、皱木耳、紫木耳菌丝生长的最适温度范围以及最佳碳源和氮源。     

B细胞淋巴瘤-2家族蛋白与细胞凋亡简

细胞凋亡是由多基因控制的细胞自主有序的死亡,以维持内环境稳定。其在多细胞生物的组织分化、器官发育、机体稳态的维持中有重要意义。细胞凋亡可促进因感染、损伤所致的细胞死亡并被机体清除,临床上细胞凋亡与许多疾病有直接关系。B细胞淋巴瘤-2（Bcl-2）家族蛋白是参与细胞凋亡的重要调节分子,既可抑制也可促进细胞凋亡。作者概述了Bcl-2蛋白家族成员、结构特点、Bcl-2蛋白家族与细胞凋亡的关系及生物学意义。     

牛粪中纤维素分解菌的分离鉴定及其产酶条件优化研究

A strain of highly cellulolytic decomposing bacteria was obtained by the congo red flat plate straining and shake flasking method screened from the ox dung which named N12. It had been identified as Bacillus which belonged to Bacillus pumilus sp. by morphologic characteristics observation,physiology and biochemical experiment and also 16S rRNA genetic analyze.The conditions of producing cellulase on strain N12 were also been studied in this test.When the contents of carbon and nitrogen sources were 0.5% maizena and 1% yeast powder,and also the nitial pH was 3.0,the fermentation temperature was 42 ℃,at the same time the fermentation time was 54 h,it had the highest enzyme activity.     

微量元素锌在动物生产中应用研究新进展

Zinc as a microelement is essential for animal body,the author reviewed recent research progress on application of zinc in animal production at home and abroad,combined with the zinc distribution and existing form in animal body,summarized the biological function of zinc and its application in animal production,respectively. The paper not only provided a basis for better utilization of zinc and its mechanism in animal production,but also put forward the problems and prospect of zinc in animal husbandry.     

猪繁殖与呼吸综合征病毒核衣壳蛋白与糖蛋白的表达纯化及鉴定

In order to gain nucleocapsid protein （N） and glycoprotein （E） of porcine reproductive and respiratory syndrome virus （PRRSV）, the total RNA was extracted,ORF7 and ORF5 genes were obtained by RT-PCR, inserted into pET-28a(+) vector, and then transformed into Escherichia coli BL21（DE3）,respectively. The expressed products were identified by SDS-PAGE and Western blotting, and purified by affinity chromatography. The results showed that N and E fusion protein were successfully expressed and the proteins had good reactionogenicities by Western blotting analysis. The purities of the purified proteins were 89% and 90%, respectively. This study could lay foundations for molecular biological function research and establishment of test methods for detection antibodies of PRRSV.     

吉林省水稻品种选育过程及品种的农艺性状和生理特性的变化

20世纪以来,吉林省水稻品种经历了五次更新,使2011年吉林省水稻单产达到535.0 kg/667 m2。本文通过对吉林省水稻育种历史的回顾和总结,结合前人研究结果及本课题组近年来对吉林省历史育成品种的农艺性状和生理特性进行的系统研究,提出吉林省下一步超级稻育种的目标株型及农艺性状特点。同时建议为提高品种选育的科学性,可将水稻生理机能纳入育种选择的范畴,水稻上3叶光合能力特别是倒3叶光合能力可作为生理机能的重要参考。     

玉竹不同品系多糖含量分析比较

目的通过对八个品系供试玉竹多糖进行比较分析,为玉竹种质资源的搜集、玉竹的育种和规模化栽培提供基础资料。方法本试验在相同条件下对八个品系供试玉竹进行了栽培,进行了多糖含量提取及测定。结果吉竹一号叶腋紫、吉竹一号茎紫在本地栽培后的多糖总量含量最高,分别为15.49%和15.03%。结论吉竹一号叶腋紫和吉竹一号茎紫与其它六个品系玉竹相比更适合在吉林地区进行种植。     

不同诱导子对王不留行毛状根生长和王不留行黄酮苷含量的影响

目的研究水杨酸、茉莉酸甲酯、酵母提取物这三种生物诱导子对王不留行毛状根生长及王不留行黄酮苷含量的影响。方法利用高效液相色谱法测定毛状根及培养基中王不留行黄酮苷的含量。结果水杨酸和酵母提取物对王不留行毛状根生长的影响不显著,茉莉酸甲酯则明显抑制其生长。这三种诱导子均明显促进王不留行黄酮苷的产生。结论为进一步筛选适宜的王不留行毛状根培养体系并调控次生代谢产物的研究奠定了基础。