禾谷镰刀菌培养性状与致病力的相关性分析

选择来自我国12省市有代表性的101株禾谷镰刀菌菌株,比较它们在PDA培养基上的表型性状以及在CMS液中的产孢量;并于田间小麦开花期,用单花剪滴法接种3个抗赤霉病性不同的小麦品种,鉴定其致病力,用SAS软件进行统计分析。结果表明,菌落扩展速度不同的菌株问,其致病力差异显著;相关分析证实,菌落扩展速度与致病力之问呈极显著线性正相关(P=0．0003),菌丝生长状况与致病力之间呈极显著非线性相关(P=0．0083),而基质颜色和产孢量与致病力没有相关性。这些结果说明,禾谷镰刀菌菌落的扩展速度及菌丝生长状况与致病力之间具有共同的遗传基础或遗传相关性。     

加拿大披碱草-野大麦三倍体杂种加倍植株同工酶分析

分析了加拿大披碱草-野大麦三倍体属间杂种F₁加倍植株的同工酶酶谱特征。结果显示:同一生育阶段杂种自然加倍植株与加倍F₁代植株在EST、POD和SOD酶带的数目、位点及强弱方面具有一致性和遗传稳定性,而与杂种F₁代及亲本的酶带表型差异显著,从酶蛋白分子水平证明该杂种F₁染色体加倍是真实的;加倍植株抽穗期旗叶的EST、POD酶谱中分别呈现9和4条酶带,分蘖期幼叶的EST、POD、SOD酶谱内分别呈现8、4和4条酶带,有多态性位点的特征酶带可作为杂种加倍后代育性恢复鉴定的遗传标记的候选位点;对供试材料不同生育阶段做同工酶酶谱对比分析,比单一生育阶段更能反映其酶带表型的遗传差异性,提高同工酶电泳技术鉴定结果的准确性。     

基于BERT的水稻表型知识图谱实体关系抽取研究

针对水稻表型知识图谱中的实体关系抽取问题，根据植物本体论提出了一种对水稻的基因、环境、表型等表型组学实体进行关系分类的方法。首先，获取水稻表型组学数据，并进行标注和分类；随后，提取关系数据集中的词向量、位置向量及句子向量，基于双向转换编码表示模型（BERT）构建水稻表型组学关系抽取模型；最后，将BERT模型与卷积神经网络模型、分段卷积网络模型进行结果比较。结果表明，在3种关系抽取模型中，BERT模型表现更佳，精度达95.11%、F1值为95.85%。     

番茄生长表型对棉秸秆复合育苗基质理化性质的响应

研究棉秸秆复合育苗基质的理化性质及其与番茄生长表型的关系,旨在为合理高效利用秸秆资源提供理论基础。以棉秸秆复合育苗基质理化性状为自变量,以番茄幼苗生长表型为因变量进行通径分析。腐熟后的棉秸杆在添加AM菌土、菇渣+烟渣混合物和蛭石等基料后可显著提高番茄种子的萌发率(P<0.05);T4（棉秸秆:AM菌土:珍珠岩=3:0.5:1）处理番茄幼苗质量最优且与CK (PINDSTRUP)无显著差异性(P>0.05),棉秸秆混配其他基料后可显著提高容重、降低EC值(P<0.05);通径分析表明,容重、总孔隙度、pH、EC值是影响番茄生长表型的主要因子,其中通气孔隙和持水空隙对其生长表型是协同、促进作用,而其他因素的作用是负向的。适量增加棉秸秆复合育苗基质的通气孔隙和持水空隙,降低容重、pH以及EC值有利于培育番茄壮苗。     

Molecular characterization and pathogenicity of Francisella noatunensis subsp. orientalis isolated from cultured tilapia (Oreochromis sp.) in Taiwan

Francisella noatunensis subsp. orientalis is a causative agent of systemic granulomatous disease in tilapia. The present study was designed to understand the genetic and phenotypic diversities among Taiwanese Fno isolates obtained from tilapia (n = 17) and green Texas cichlid (Herichthys cyanoguttatus) (n = 1). The enzymatic profiles of the isolates were studied using the API ZYM system. Phylogenetic tree analysis of the 16S rRNA and housekeeping gene and pulsed‐field gel electrophoresis (PFGE) were carried out to determine the genotypic characters of all isolates. The phylogenetic tree showed similarity of 99%–100% nucleotide sequences of 16S rRNA and housekeeping genes compared to the Fno references genes from GenBank database. Comparatively, the results revealed an identical profile of enzymatic and PFGE pattern which was distincted from that of F. philomiragia. To understand the pathogenicity, the isolates were intraperitoneal injected to tilapia the gross lesions were observed concomitant with natural outbreak. Median lethal dose upon Nile tilapia and red tilapia were 9.06 × 10³ CFU/fish and 2.08 × 10² CFU/fish, respectively. Thus, our data provide understanding the epidemiology of Taiwanese Fno isolates, and help in development of future control and prevention.     

禾谷镰孢菌FgCYP51B蛋白Y137H影响分生孢子及子囊孢子发育

<正>小麦赤霉病(Fusarium Head Blight,FHB),主要是由镰刀菌属真菌引起的一种流行性毁灭性病害。在世界各小麦种植区尤其是温暖潮湿和半潮湿地区发生最为严重~([1,2])。它不仅能够降低小麦产量,其染病麦粒中还会产生对人畜健康严重危害的真菌毒素~([3])。在不同的地区由于气候因素和地理环境的不同,分布着不同的致病菌株。在我国,     

甘肃省扎尕梁北坡头花杜鹃枝叶性状特征北大核心CSCD

高山植物在适应高海拔、低气压、短生长季等环境约束的过程中表现出独特的功能适应性,探究高山植物枝叶功能性状沿海拔梯度的变化特征对其资源获取与生存繁殖的认知有重要意义。于2019年8月对甘南高山海拔3400~3700 m范围内头花杜鹃23组主要枝叶功能性状进行了测定。研究发现:随海拔上升,叶干物质含量、叶片密度、叶厚度和总叶干物质含量显著增大;叶长、叶宽、叶面积、叶体积、叶干重、茎长、茎干重、总叶干重、总叶面积、纤细率和茎体积在3600 m显著减小,在海拔梯度上整体呈"V"形变化;海拔会显著影响叶厚、叶长、叶干物质含量、叶密度、比叶面积、总叶干物质含量、总叶面积和总比叶面积,大部分性状间存在显著的相关关系;头花杜鹃枝叶性状在海拔内的方差分量明显大于海拔间,各性状的表型分化系数以茎干物质含量最小(3.19%),叶密度最大(57.73%),平均为20.65%。结果表明:头花杜鹃部分性状对海拔变化不敏感,主要以少数核心性状变异结合功能性状组合共同适应高山异质生境,头花杜鹃在海拔间具有较低的表型变异,可能是对高山特殊环境的适应性策略。     

Phenotype of decidual NK cells in women with hypertensive disorder complicating pregnancy

AIM: To investigate the phenotype of uterine decidual natural killer cells (dNK cells) in women with hypertensive disorder complicating pregnancy (HDCP). METHODS: All the study subjects were collected from Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China. Twenty cases of singleton pregnancy who underwent caesarean section because of HDCP were selected as HDCP group, and 15 cases of singleton pregnancy received selective caesarean section because of social-psychological concerns were also randomly selected as normal control group. The decidual tissues were sampled immediately after caesarean section. The mononuclear cells were extracted from the tissues by means of mechanic grinding and gradient centrifugation. The technique of flow cytometry was used for dNK cell sorting and the expression of CD56 and CD16 on the surface of cells was also examined. RESULTS: In HDCP group and normal control group, the proportions of CD56^brightCD16^-CD3^- dNK cells were significantly higher than those of CD56^dimCD16⁺CD3^-dNK cells (P<0.01). Neither the CD56^brightCD16^-CD3^- subset nor the CD56^dimCD16⁺CD3^- subset had statistical difference between HDCP group and normal control group. No significant difference of CD16 expression on the surface of dNK cells between HDCP group and normal control group was observed (P>0.05). CONCLUSION: The phenotypes of dNK cells from the women with HDCP and from healthy pregnant women are both dominated by CD56^brightCD16^-CD3^- subset, without significant difference.     

北京油鸡羽速基因分子检测及其对生产性能的影响

通过对北京油鸡雏鸡进行羽速表型判定,并与ev21基因插入位点分子检测结果比对,二者之间一致性达到99%,证实羽速基因在北京油鸡群体中属于性连锁基因,具备建立自别雌雄配套系的遗传基础.对不同羽速类型北京油鸡母鸡生产性能分析发现,不同羽型油鸡母鸡8-22周双周末体重差异不显著(P>0.05),42～44周龄总产蛋数以及相同...