五种牧草及三种草坪草种子寄藏真菌检测初探

采用PDA平皿法和滤纸法检测了市售5个牧草品种和3个草坪草品种种子样品寄藏真菌的情况,并就分离获得的真菌对种子出苗及幼苗生长的影响进行了初步研究.结果表明,供试种子样品带菌率为0～70.5%,分离获得的35个真菌分离物大多属于镰刀菌(Fusarium spp.)、链格孢菌(Alternaria spp.)、蠕孢菌(Helminthosporium spp.)、小核菌(Sclerotium sp.)、匍柄霉菌(Stemphylium spp.)和曲霉菌(Aspergillus spp.).供试样品中假俭草和波多马各(鸭茅)种子带菌率较高,在PDA平皿检测中其未经表面消毒处理的种子带菌率分别为48.4%和70.5%,表面消毒处理种子带菌率分别为40.8%和28.8%,分离获得的种子寄藏真菌对假俭草和波多马各种子出苗及幼苗生长的影响较小.圣地亚哥(南苜蓿)和菊苣种子带菌率较低,但分离获得的真菌对其种子出苗和幼苗生长的影响较大.以镰刀菌与匍柄霉菌接种过的南苜蓿种子出苗率分别比未经表面消毒且未接种真菌的对照低44.0%和21.7%,出苗后的病苗率分别为22.7%和29.7%;以链格孢菌与曲霉菌接种过的菊苣种子的出苗率分别比未经表面消毒且未接种真菌的对照低22.0%和27.5%,出苗后的病苗率分别为2.3%和29.3%.     

农用电机保护装置与仪表一体化研究

在分析农用电机烧损现象及研究现有保护方式的基础上,提出了选频振荡电路通过仪表的表针摆动实现采样、控制、保护的新方法,并研制了保护元件与仪表一体的装置,实现了对运行设备既能监视又能起到保护作用的一体化装置。     

甜樱桃四倍体杂种砧木Y1高频、高效离体再生体系研究

以甜樱桃四倍体矮化砧木Y1试管苗叶片为外植体,探讨了不同基本培养基、激素组合、暗培养时间和叶片发育阶段对其不定芽再生的影响,建立甜樱桃四倍体矮化砧木的高频、高效离体再生体系,为进一步进行遗传转化研究奠定基础。结果表明,甜樱桃四倍体矮化砧木Y1采用WPM培养基再生效果最好,明显优于MS和DKW培养基;最佳激素组合为6-BA2.0 mg.L-1+IBA1.0 mg.L-1;接种后暗培养可以明显提高不定芽再生率,最适宜暗培养时间为14 d;Y1试管苗顶部完全展开的幼嫩叶片再生能力最高。通过以上条件的优化,成功建立了甜樱桃四倍体矮化砧木的高频、高效离体再生体系,离体叶片不定芽再生率达90%,每叶片平均再生不定芽数达4.1。     

挤奶频率对奶牛生产性能的影响

文章介绍了挤奶频率对奶牛泌乳性能、繁殖性能、健康与福利等方面的影响。     

Response of Maize and Cowpea Grown Sequentially to Application of Phosphate Rock in the Humid Tropics

Field trials were conducted at the Institute of Agricultural Research and Training Ibadan in 2000 and 2001 cropping seasons to evaluate the efficiency of some phosphorus (P) sources, rates, and frequency of application on grain yields of maize and cowpea grown sequentially. The experiment was laid out in a split–split plot arrangement using randomized complete block design, with three replications. The main plot was frequency of fertilizer application (seasonal and annual). Source of P [single superphosphate (SSP: 18% P₂O₅)]; Ogun rock phosphate (OPR: 20.21% P₂O₅), and crystallizer super (CS: 31.4% P₂O₅) constituted the subplots. The P rates (0, 20, 40, and 60 kg P ha^?1) were the sub-subplots. An early maize cropping followed by a late-season cowpea was the sequential cropping, while an early maize followed by a late-season maize was the continuous cropping. The croppings were repeated the second year. Frequency of P application and source of P were significantly different while rates of application were not. Maize and cowpea grain yields from the control treatment were significantly less than yields from P-treated plots. Maize grain yields were slightly greater in plots alternated with cowpea, compared to continuous maize. Relative agronomic efficiency (RAE) values of OPR and CS were 67% and 75%, respectively, in annual application and 140% and 134% in seasonal applications, respectively, in 2000. In 2001, the trend was almost similar to 2000 except that there was a decline in RAE values of cowpea. Each Naira invested in the annual application returned about ?3.12 while about ?2.13 was returned with the seasonal application (?125.00 is the equivalent of USD$1.00). The OPR and CS can serve as alternatives to water-soluble sources such as SSP.     

变频调速系统控制方法的研究

针对传统变频调速控制系统的不足,本文在交―交变频调速系统的基础上,引入了一种新的控制方法———对称余弦控制法。这种控制方法不但兼顾了传统控制方法所固有的正弦性,同时又由于它自身控制方式的特点,能很好地解决波形的对称性问题,同时兼顾输出波形的对称度与正弦度。通过仿真实验,该控制方式能很好地兼顾对称度和正弦度,输出的谐波含量也较小,取得了良好的效果。     

Establishment of Efficient Regeneration System of Populus×euramericana cl.‘Bofeng’

Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L^-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L^-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper（Cu） in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials.     

台湾中部地区短延时降雨强度初步探讨

台湾地区在经历1999年的921地震后,山坡地土体松动严重,仅仅短延时的降雨即有机会引起土石流的发生,而近年微小的降雨条件下即有可能触动土石流发生,因此将台湾中部地区14个测站所测得的台风降雨资料,依照台风降雨强度进行水文频率分析,分析方法采用对数常态分布法、极端值第一类分布法、皮尔逊第三类分布法及对数皮尔逊第三类分布法等4种方法,计算短延时重现期距并绘成降雨强度-延时-频率之分析曲线,再整合各区域土石流潜势溪流的分布情况,分析台风降雨对台湾中部地区所造成之影响.     

动力优化法设计船体板架的计算结果分析

对文献“船体板架动力优化设计”一文中船体板架动力优化的计算结果重新作了分析。在目标函数及优化变量选择方面得出了不同的结论。根据船体板架固有频率的特点，提出了使其有效地避开频率禁区的措施。     

牛瘤胃胃电频谱分析中窗函数的选择

采用三种常用的窗口函数，对牛瘤胃４ 种生理状态下胃电（峰电位和慢电位）的频谱进行了分析比较，得出了不同窗函数下频谱中幅值的特性，并分析确定了作者课题组频谱分析时的窗口函数——汉宁窗。