利用类病毒脂质体抗原检测H5N1亚型禽流感病毒抗体ELISA方法的建立

利用昆虫杆状病毒表达系统制备了H5N1亚型禽流感病毒(AIV)HA蛋白、类病毒脂质体和病毒样颗粒,分别作为包被抗原,建立了相应的检测H5N1亚型禽流感病毒抗体的ELISA方法。特异性试验、敏感性试验、重复性试验和稳定性试验结果表明,利用3种抗原包被所建立的ELISA方法均具有良好的重复性和稳定性,批间和批内变异系数均小于10%,但单独表达的HA蛋白和类病毒脂质体特异性更好,而且类病毒脂质体有更高的免疫反应性,与灭活全病毒相比安全性更高,故在H5N1亚型AIV抗体水平检测方面更具有应用前景。     

木材初期腐朽研究综述

木材容易受到各种微生物的侵袭,真菌腐朽是导致木材破坏最严重的一种方式,即使是在木材质量损失率很小的腐朽初期,真菌也可以迅速引起木材结构的破坏,导致木材强度的急剧降低.生物培养和显微镜观察被认为是目前唯一权威的用来检测和评估木材初期腐朽的方法,但这些方法很难对木材的初期腐朽进行快速、准确地评估.因此,寻找一种迅速、准确地检测和评估木材初期腐朽的方法倍受人们的关注.有关初期腐朽及其检测与评估的研究在国外已有大量报道,而在我国却极为少见.本文综述了近几十年国内外有关木材初期腐朽及其检测与评估的研究,旨在增强人们对木材初期腐朽危害的认识,并呼吁有关部门重视相关研究在我国的发展.     

两种热带森林覆盖变化检测方法比较研究

[目的]以热带森林复杂区域为对象,对两种缨帽变换“衍生数据”检测热带森林变化的方法的优劣进行比较.[方法]对两期数据进行缨帽变换,同时结合黑暗对象掩膜与局部直方图阈值提取等方法,获取亮度、绿度、湿度指数组合(MKT)和干扰指数(DI).采用MKT差值(MKT-D)、干扰指数差值(MDI-D)进行变化信息识别,然后,根据植被覆盖与亮度、绿度、湿度之间的变化关系,通过决策树分类提取变化信息,最后,对不同检测结果进行验证与比较分析.[结果]结果显示两种方法都能检测出森林内部的细微变化,但对小图斑变化,MKT-D检测优势明显,且MKT-D的kappa系数为0.763 0,MDI-D的kappa系数为0.655 9,两者相比,MKT-D方法优于MDI-D方法.[结论]MKT能够增强短波红外与近红外波段对森林变化信息的敏感性,有效地消除噪声等非目标信号,突出目标信号,此外,MKT-D为RGB彩色图像,更利于变化信息的目标提取与解译.     

四种桉树青枯菌DNA提取方法及PCR检测灵敏度比较

以带有青枯菌Ralstonia solanacearum的桉树组织为材料,采用4种不同的提取方法抽提青枯菌DNA,同时利用青枯菌的特异性引物进行PCR扩增,比较了4种DNA的提取方法及PCR检测的灵敏度。结果表明,煮沸法和热裂解法操作相对简单,但检测灵敏度较低,检测限分别为104CFU/mL和103CFU/mL;简化提取法和试剂盒法检测效果较好,均可检测到102CFU/mL的青枯菌;简化提取法比试剂盒法操作更简单,检测成本低,具有更高的应用价值。     

An “electronic nose” as a potential device for fire detection of forest product fire loads in enclosures

Abstract

Saving lives and properties are of primary importance in fire disasters. Unfortunately, current fire detectors are not fast enough to allow the detection of many fires, especially, at the smouldering stages. Some preliminary studies on laevoglucosan (LG), as an important product from cellulose degradation, have shown interesting properties and could be detected by devices classified as “electronic noses” (e-noses). In the present work, the mechanisms of pyrolysis of some ligno-cellulosic materials were investigated using thermo-gravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy and by a tube furnace with a view to determining the major degradation products from wood, white paper and cardboard samples. Additionally, an odour sensor, made of tin oxide semiconductor thin film, was employed to evaluate its sensitivity in detecting LG. It was found that LG can be readily found among the thermal degradation products of cellulosic materials in question, and that the metal oxide sensor employed was sufficiently sensitive in detecting its presence. Therefore, the work opens up the potential of using such a sensor as an “e-nose” for the early detection of fires stemming from forest product fire loads in storage places and in other enclosures.     

卫星遥感技术在林火管理与研究中的应用(英文)

卫星遥感已经成为森林火险等级预测、可燃物和火烧区制图、林火监测和火生态研究的一个主要数据来源。本文综述了这些研究领域的研究成果,分析了未来林火管理中采用的卫星遥感技术的发展趋势。根据卫星遥感数据制取的可燃物分布图可以满足林火管理在空间和时间尺度上的需要。单独采用遥感数据或结合地面气象数据可以生成一些火险指数,用于森林火险的预报。目前NOAA 和MODIS 卫星由于有高的时间分辨率已被广泛用于林火探测和监测,这些监测结果可以在许多林火网站上见到,这为世界各地的林火管理和研究提供了重要的参考资料。作为低成本的有效工具, 卫星遥感技术在确定火烧面积和过火区制图上发挥了重要作用。遥感技术的发展也可以用来推断火烧时间和估计火烧程度。卫星遥感也非常适合用来估计生物燃烧面积,这是估计全球或区域生物燃烧排放量和理解火对全球变化的影响的基础。本文还讨论了林火研究中采用的卫星类型。文章最后建议中国需要在卫星遥感技术的应用上进一步发展,提高我国的林火管理水平。参71。     

Decay Detection of Ancient Wood Rafters in Tibet by Stress Wave Testing Method

Visual classification and stress wave non-destructive testing technology were adopted to evaluate the decay status of ancient Populus wood members （rafters） replaced from the Potala Palace. The decay status of wood members was evaluated by stress wave testing and visual observation. For most of the ancient wood members, the evaluation results by two methods were consistent with each other. Also stress wave testing techniques can find the internal wood member decay to eliminate the hidden hazard for ancient wood members, and offer relatively accurate quantitative information for the safety status of ancient wood members. Thus during the maintaining for ancient architectures, visual observation combined with stress wave testing techniques is a good way to evaluate the degradation of ancient wood members.     

Determination of isoflavones in red clover by capillary electrophoresis with electrochemical detection

Capillary electrophoresis with electrochemical detection (CE-ED) was employed to analyze isoflavones in red clover (Trifolium pratense). The effects of potential of working electrode, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits a good response at +0.85 V (versus saturated calomel electrode) for the analytes. The analytes could be separated in a 50 mmol/l borate buffer (pH 9.5) within 25 min. The response was linear over three orders of magnitude with detection limit (S/N=3) ranging from 2x10(-5) mg/ml to 5x10(-5) mg/ml for the analytes. This method has been used for the determination of daidzein, genistein and biochanin A in red clover with satisfactory results.     

图像分割技术在木材表面缺陷识别中的应用

阐述了图像分割技术在图像处理及分析中重要意义,以及主要的图像分割技术。以木材表面缺陷为主要研究对象,利用微分算子边缘检测、最优迭代阈值分割及形态学方法针对具有代表性缺陷死节和虫眼进行分割处理。