BSE‐associated polymorphisms in the prion protein gene: an investigation

The aim of this study was to determine the frequency of the 12‐bp and 23‐bp indel polymorphisms in the prion protein gene (PRNP) in cattle and to investigate the association between these frequencies and the occurrence of bovine spongiform encephalopathy (BSE). There was no significant difference in the 12‐bp indel frequency between the BSE animals and control group. For the 23‐bp indel, the BSE animals had a significantly lower + + (insins) genotype frequency and + allele frequency compared with the control animals. The ? ? / ? ? genotype frequency in the BSE animals was not significantly higher when compared with the control animals. One ? allele increased the risk of BSE by a factor of 1.55 (i.e. by 55%) for the 12‐bp indel and by a factor of 2.10 for the 23‐bp indel. When both indels are considered, one ? allele increased the risk of BSE by a factor of 1.54.     

Immunogenicity and efficacy of a bivalent vaccine against infectious bronchitis virus

Infectious bronchitis (IB) is a highly contagious viral disease and is responsible for considerable economic losses in the poultry industry, worldwide. To mitigate the IB-associated losses, multiple vaccines are being applied in the sector with variable successes and thus necessitating the development of a potent vaccine to protect against the IB in the poultry. In the present study, we investigated a bivalent live attenuated vaccine consisting of IB virus (IBV) strain H120 (GI-1 lineage) and D274 (GI-12 lineage) to evaluate its protection against heterologous variant of IBV (GI-23 lineage) in chicken. Protection efficacy was evaluated based on the serology, clinical signs, survival rates, tracheal and kidney histopathology and the viral shedding. Results demonstrated that administering live H120 and D274 (named here Classivar®) vaccine in one day-old and 14 days-old provided 100 % protection. We observed a significant increase in the mean antibody titers, reduced virus shedding, and ameliorated histopathology lesions compared to routinely used vaccination regimes. These results revealed that usage of different IBV vaccines combination can successfully ameliorate the clinical outcome and pathology in vaccinated chicks especially after booster vaccination regime using Classivar®. In conclusions, our data indicate that Classivar® vaccine is safe in chicks and may serve as an effective vaccine against the threat posed by commonly circulating IBV strains in the poultry industry.     

转Xa21基因圣稻301的培育及其田间表现

利用农杆菌介导的遗传转化系统 ,将白叶枯病抗性基因Xa2 1导入山东主栽品种圣稻 30 1胚性愈伤组织 ,从T0 至T5代 ,利用GUS染色和分子标记辅助逐代选择并进行田间抗病虫性鉴定 ,获得了大量纯合稳定的转基因圣稻 30 1,并对其田间抗性及农艺性状表现进行了调查研究     

春小麦新品种陇春23选育报告

春小麦新品种陇春 2 3 ( CM4 86 0 )是 1 998年从国际玉米小麦改良中心 ( CIMMYT)引进的材料中经鉴定、筛选育成的一个春小麦新品种。该品种具有丰产、稳产性好、品质优、抗条锈、抗倒性强、适应性较好等特点 ,在 2a的甘肃省东片水地春小麦区域试验折合平均产量 5 6 6 9.2 kg/ hm2 ,较对照品种陇春 1 5增产 1 2 .73 % ,在生产示范中折合平均产量 5 2 5 4 .5 kg/ hm2 ,较当地对照品种增产 4 1 .70 % ,均居第 1位。该品种高抗条锈病、中抗白粉病 ,属中筋优质小麦 ,适宜在甘肃省中部的临洮、兰州、定西、临夏及沿黄灌区以及生态条件相似的地区种植     

枸杞多糖对免疫抑制小鼠血清中IL-6、IL-10和IL-12/IL23 p40分泌水平的影响

通过研究枸杞多糖(LBP)对免疫抑制小鼠血清中细胞因子IL-6、IL-12/IL-23p40和IL-10分泌的影响,探讨LBP的免疫调节机制,为LBP的开发利用提供理论依据。60只ICR雌性小鼠,随机分为5组,对各组小鼠进行连续4d每日腹腔注射环磷酰胺(40mg/kg),间隔3d后,再连续4d每日腹腔注射环磷酰胺(40mg/kg);同时,用高(40mg/1kg)、中(20mg/1kg)、低剂量(10mg/1kg)的LBP给小鼠连续灌胃2周,阳性对照和阴性对照分别用LPS(1mg/kg)和PBS,连续2周。小鼠眼眶取血,分离血清,ELISA检测血清中IL-6、IL-12/IL-23p40和IL-10的分泌水平。结果显示,与阳性对照和阴性对照组相比,LBP能提高免疫抑制小鼠血清中IL-6、IL-12/IL-23p40和IL-10的分泌水平,提示LBP能增强小鼠细胞免疫与体液免疫应答,为枸杞多糖作为免疫增强剂的开发应用奠定了一定的理论基础。     

Sinomenine induces apoptosis of human rheumatoid arthritis fibroblast-like synoviocytes via miR-23b-3p/FGF9 signaling pathway

AIM To investigate the effect of sinomenine （SIN） on the apoptosis of human rheumatoid arthritis （RA） fibroblast-like synoviocytes （FLS） and its molecular mechanism. METHODS Human RA FLS were isolated and cultured. The cells treated with lipopolysaccharide （LPS） at 100 mg/L was recorded as LPS group. The cells treated with SIN at 3.2 mmol/L and LPS at 100 mg/L were recorded as LPS+SIN group. The cells without any treatment served as blank group. The cells transfected with miR-con， miR-23b-3p， si-con and si-fibroblast growth factor 9 （FGF9） and treated with 100 mg/L LPS were recorded as LPS+miR-con group， LPS+miR-23b-3p group， LPS+si-con group and LPS+si-FGF9 group， respectively. The anti-miR-con， anti-miR-23b-3p， pcDNA and pcDNA-FGF9 were also transfected into RA FLS， and then the cells were treated with SIN at 3.2 mmol/L and LPS at 100 μg/mL. These cells were recorded as LPS+SIN+anti-miR-con group， LPS+SIN+anti-miR-23b-3p group， LPS+SIN+pcDNA group， LPS+SIN+pcDNA-FGF9 group， respectively. The cell viability was measured by CCK-8 assay. The number of colonies was accessed by colony formation experiment. The protein levels of FGF9， cleaved caspase-3， Bax and Bcl-2 were determined by Western blot. The apoptosis was analyzed by flow cytometry. The expression of miR-23b-3p and FGF9 mRNA was detected by RT-qPCR. Dual-luciferase reporter assay was used to verify the targeting relationship between miR-23b-3p and FGF9. RESULTS Treatment with SIN promoted LPS-induced apoptosis of RA FLS， inhibited cell proliferation， up-regulated miR-23b-3p expression， and down-regulated FGF9 expression. miR-23b-3p targeted FGF9. Over-expression of miR-23b-3p or silencing of FGF9 inhibited LPS-induced proliferation and enhanced apoptosis of the RA FLS. Interfering with miR-23b-3p or over-expression of FGF9 reversed the effects of SIN on the proliferation and apoptosis of LPS-induced RA FLS. CONCLUSION Sinomenine induces RA FLS apoptosis and inhibits cell proliferation through miR-23b-3p/FGF9 signaling.     

不同种植密度对玉米郑单23产量和生理指标的影响

以郑单23为试验材料,在30000、37500、45000、52500、60000、67500和75000株/hm2种植密度下,研究了种植密度对郑单23玉米品种产量和生理指标的影响.结果表明:郑单23的产量随着种植密度的增加先增后减,产量在52500株/hm2时最高,达9141.67kg/hm2;在30000～75000株/hm2的密度范围内,随着种植密度的增加,玉米郑单23的株高、穗位升高,单株叶面积和单株干物质积累量减小,单株产量明显降低,经济系数表现为减-增-减的变化趋势.     

甘蔗CIPK23基因克隆及对低钾、干旱、盐胁迫的响应

本研究采用RT-PCR技术从低钾胁迫的甘蔗根系中克隆得到一个长度为1 749 bp的与CBL互作的丝氨酸/苏氨酸蛋白激酶基因,命名为Ss CIPK23(Gen Bank登录号为KM981737),包含1 350 bp的开放阅读框(ORF),可编码一个449氨基酸残基的多肽。Ss CIPK23具有CIPK基因家族典型的蛋白激酶结构域和NAF调控结构域。进化树分析显示Ss CIPK23与其他作物的CIPK23关系较近,具有较高的保守性。q PCR分析结果表明,在低钾、干旱和盐胁迫下,Ss CIPK23的表达都发生改变。在低钾胁迫24 h后Ss CIPK23相对表达量开始上升,而且随着胁迫时间的延长,相对表达量持续增加;而在干旱和盐胁迫下,相对表达量最高出现在48 h,随后表达量开始下降,暗示了该基因在低钾、干旱和盐胁迫下发挥重要的调控作用,可为深入研究Ss CIPK23基因功能奠定坚实的基础。     

牛瑟氏泰勒虫P23基因的克隆与生物信息学分析(英文)

[Objective] The aim of this study is to provide basis for developing genetic engineering vaccine and diagnostic kit for Theileria sergenti infection. [Objective] P23 gene of Theileria sergenti was amplified from its genomic DNA by PCR amplification, and cloned into the pGEM-Easy vector; then the sequencing result was analyzed with bioinformatics methods. [Result] Whole length of the P23 gene from Theileria sergenti is 684 bp containing a 672 bp open reading frame. The deduced amino acid sequence (223 amino acid residues) contains a signal peptide of 19 amino acid residues and two fragments of transmembrane domains, with relative molecular weight of the 25.886 kD and with the pI of 9.22. The homology between the yielded sequence and Chitose of Theileria sergenti P23 gene(TS-Chitose type, D84446), Ikeda of Theileria sergenti P23 gene(TS-Ikeda type, D84447) reached 99% and 90%, respectively. The sequence has been accessed in GenBank(EU573168). [Conclusion] The protein encoded by the P23 gene has better stability and immunogenicity, thus can be used as the antigen candidate for preparing genetic engineering vaccine for Theileria sergenti.     

Pharmacokinetics and pharmacodynamics of a therapeutic dose of unfractionated heparin (200 U/kg) administered subcutaneously or intravenously to healthy dogs

BACKGROUND: Heparin treatment has been recommended for dogs in hypercoagulable states such as disseminated intravascular coagulation, however, potential benefits have to be balanced against the bleeding risk if overdosage occurs. A better understanding of the pharmacology of heparin and tests to monitor heparin therapy in dogs may help prevent therapeutic hazards. OBJECTIVES: The purpose of this study was to evaluate the effects of 200 U/kg of sodium unfractionated heparin (UFH) on coagulation times in dogs after intravenous (IV) and subcutaneous (SC) administration and to compare these effects with plasma heparin concentrations assessed by its antifactor Xa (aXa) activity. METHODS: 200 U/kg of UFH were administered IV and SC to 5 healthy adult Beagle dogs with a washout period of at least 3 days. Activated partial thromboplastin time (APTT), prothrombin time (PT), and plasma aXa activity were determined in serial blood samples. RESULTS: After IV injection, PT remained unchanged except for a slight increase in 1 dog; APTT was not measurable (>60 seconds) for 45-90 minutes, and then decreased gradually to baseline values between 150 and 240 minutes. High plasma heparin concentrations were observed (maximal concentration = 4.64 +/-1.4 aXa U/mL) and decreased according to a slightly concave-convex pattern on a semilogarithmic curve, but returned to baseline slightly more slowly (t240-t300 minutes) than did APTT. After SC administration, APTT was moderately prolonged (by a ratio of 1.55 +/-0.28 APTT t0, range 1.35-2.01) between 1 and 4 hours after administration. Plasma aXa activity reached a maximum of 0.56 +/-0.20 aXa U/mL (range 0.42-0.9 U/mL) after 132 +/-26.8 minutes; this lasted for 102 +/-26.8 minutes. Prolongation of APTTs of 120-160% corresponded to plasma heparin concentrations of 0.3-0.7 aXa U/mL. CONCLUSIONS: As in humans, the pharmacokinetics of UFH in dogs was nonlinear. Administration of 200 U/kg of UFH SC in healthy dogs resulted in sustained plasma heparin concentrations in accordance with human recommendations for thrombosis treatment or prevention, without excessively increased bleeding risks. In these conditions, APTT can be used as a surrogate to assess plasma heparin concentrations. These findings need to be confirmed in diseased animals.