阿留申病循环免疫复合物检测技术在水貂育种中的应用

应用 PEG沉淀法 ,对种貂进行循环免疫复合物检测 ,比较阴阳性种貂的生长状况和生产指标 ,阴性貂显著好于阳性貂 ,提出适合我国国情的针对阿留申病的水貂育种措施     

Procedure for PEG treatment of bovine sera used for cell culture in virus diagnosis

A method for PEG treatment of bovine sera to be used for cell cultures is presented. The procedure consists in repeated freezing and thawing cycles of the sera, after PEG precipitation until a clear solution is obtained. The sera, so prepared, are homogeneous in composition and appropriate for cell growth in primary cell cultures and cell lines.     

PEG诱导水分胁迫对柑桔幼苗细胞质膜透性及脯氨酸含量的影响

柑桔叶片相对含水量(RWC)随聚乙二醇(PEG)处理渗透势的降低而依次降低;叶片细胞质膜透性随PEG处理浓度的增加而依次增大;幼苗叶片累积脯氨酸的数量随水分胁迫时间和强度递增。     

Toxicity nanoinsecticide based on clove essential oil against Tribolium castaneum (Herbst)

This study aimed to characterize nanoparticles loaded with clove (Syzygium aromaticum) essential oil-based polyethylene glycol (PEG) and to know their insecticidal activity against red flour beetle (Tribolium castaneum). The nanoparticles have irregular shapes in good dispersion. The nanoformulation could not enhance clove oil contact toxicity to T. castaneum, but could protect the oil from degradation and evaporation while simultaneously allowing sustained release, as indicated by the continued high toxicity for 16 weeks of storage.     

Properties of PEG/thermally modified wood flour/polypropylene (PP) composites

In order to improve the dimensional stability of wood-polymer composites, wood flour pre-treated by polyethylene glyco1 (PEG) at two different concentrations and then thermally treated at 140°C, was used as raw material to produce wood flour/polypropylene (PP) composites at a wood content of 40%. The structure of modified wood flour was analyzed with a scanning electron microscope (SEM) and its effect on the physical and mechanical properties of wood flour/PP composites was evaluated. The SEM results indicated the "bulking" effect of PEG on wood flour, which resulted in reduced water uptake. The combination of PEG and heat treatment further improved the moisture resistance of the composites. However, PEG modification had a negative effect on the flexural modulus of rupture (MOR) and the modulus of elasticity (MOE); whereas heat treatment partly compensated for this reduction. For dynamic mechanical properties, PEG treatment decreased the storage modulus (E′). However, the heat treatment resulted in an increase of E′ of the wood flour/PP composites, with the temperature of loss factor peaks shifting to a higher temperature.     

甜菜碱不同预处理时间下小麦幼苗对PEG-6000模拟干旱的响应

以小麦长武134为实验材料,于小麦长至两叶一心期时根施2.0 g/L甜菜碱,研究外源甜菜碱预处理不同时间(48 h,72 h,96 h)后对PEG-6000(-1.0 MPa)胁迫下小麦幼苗水分状况、游离脯氨酸含量、可溶性糖含量、可溶性蛋白含量、叶绿素含量、抗氧化酶活性、膜伤害程度的影响。结果表明外施甜菜碱可增强干旱条件下细胞的渗透调节能力,提高抗氧化酶的活性,减缓干旱胁迫诱导的细胞伤害,其中用2.0 g/L的甜菜碱预处理72 h效果相对较好。     

PEG胁迫对黑麦种子萌发及幼苗生长的影响

[目的]研究聚二乙醇(PEG)对黑麦(Secale cereal L.)种子萌发及幼苗生长的影响,探讨黑麦种子耐干旱胁迫的生理机制。[方法]采用浓度分别为5%、10%、15%和20%的PEG-6000溶液模拟干旱条件对黑麦种子萌发进行胁迫。[结果]当PEG浓度为5%时,黑麦种子的发芽势和发芽率与对照相比差异不显著,干旱胁迫对发芽指数、活力指数、根长、苗长、根鲜重、苗鲜重的影响均达显著水平;当PEG浓度为10%时,各项指标受干旱胁迫的影响均达显著水平;当PEG浓度为20%时,黑麦种子不发芽。[结论]该研究可为评价黑麦种子抗旱性及黑麦的栽培管理等提供参考。     

不同浓度PEG模拟干旱胁迫对大叶铁线莲的影响

大叶铁线莲具有耐旱植物特征,根系长达60cm以上。为研究其是否能够在大庆草原沙土干旱生境应用于园林绿化,选择大叶铁线莲一年生实生苗为试材,以30%、50%、70%浓度的PEG溶液人工模拟干旱渗透胁迫,测定其在不同干旱胁迫下,叶面积、叶片含水量、根系活力的变化。结果表明:大叶铁线莲的叶面积和叶片的绝对含水量与PEG的浓度及PEG的处理时间呈负相关:其中以30%PEG处理12h叶片开始出现皱缩,叶面积减少16.67%;50%PEG处理8h减少21.69%;70%PEG处理4h减少34.00%。根系活力与PEG的浓度及PEG的处理时间呈负相关,胁迫28h,30%、50%、70%PEG处理的根系活力分别下降53.36%、72.25%和81.62%。大叶铁线莲在不同干旱胁迫下具有一定的抗旱能力,30%PEG胁迫20h以内,70%植株能恢复,PEG对恢复后的植株仍有伤害,表现为形态不正常,但能长出新叶;50%PEG胁迫16h以内,65%植株能恢复;70%PEG胁迫12h植株就失去生命活力。复水28h后,30%、50%PEG根系活力各恢复44%和14%;70%PEG处理的根系活力没有恢复。     

PEG胁迫下不同抗旱性春小麦品种的理化性质比较

以主栽品种阿勃、互助红、互麦11号、通麦1号、通麦2号为试验材料,研究了10%PEG-6000渗透胁迫对春小麦萌芽期形态、质膜透性和渗透调节物质的影响。结果表明:经10%PEG-6000胁迫处理后,5个参试小麦品种的出芽率、胚芽鞘长度、主根长、根毛数均呈下降趋势,其中通麦2号的下降幅度最小;处理组的失水率低于对照组(水),其中通麦2号的失水率最低,互麦11号的失水率最高;处理组的相对外渗电导率高于对照组,其中通麦2号的渗电导率最高,互麦11号最低。各项生理指标综合分析结果表明,通麦2号具有较强的抗旱能力。     

三种禾草萌发期抗旱性研究

采用蒸馏水及浓度为5%、10%、15%、20%和25%的PEG溶液处理梭罗草、青海草地早熟禾、垂穗披碱草种子,测定其发芽率、苗长、胚根/胚芽比值,以研究PEG对种子萌发的影响。结果显示:PEG浓度为5%时,青海草地早熟禾的相对发芽率出现小幅上升趋势,垂穗披碱草和梭罗草均呈下降趋势;不同处理对胚根/胚芽比值和苗长均有影响,梭罗草受影响小。因此这三种牧草在萌发期抗旱性依次为:梭罗草、青海草地早熟禾、垂穗披碱草。