Changes of Plasma Membrane H~+-ATPase Activities of Glycine max Seeds by PEG Treatment

The soybean (Glycine max) Heihe No. 23 is sensitive to imbibitional chilling injury. Polyethylene glycol (PEG) treatment can improve chilling tolerance of soybean seeds to a certain extent. The changes of hydrolytic ATPase in plasma membranes and H+-pumping responses in soybean seeds were investigated during PEG treatments. Effects of exogenous calcium and exogenous ABA on the hydrolytic ATPase were also examined in order to understand the mechanism of chilling resistance. Highly purified plasma membranes were isolated by 6.0% aqueous two-phase partitioning from soybean seeds, as judged by the sensitivity of hydrolytic ATPase to sodium vanadate. PEG treatment resulted in a slight increase of the hydrolytic ATPase activity in 12 h. Then the activity decreased gradually, but still higher than the control. The H+-pumping activity increased steadily during PEG treatment. Exogenous calcium had both activating and inhibiting effects on the hydrolytic ATPase, but the activity was inhibited in soybean seeds treated with exogenous ABA. Results suggested that PEG treatment, not the exogenous calcium and ABA, up-regulated H+-ATPase activities in soybean seeds.     

阿留申病循环免疫复合物检测技术在水貂育种中的应用

应用 PEG沉淀法 ,对种貂进行循环免疫复合物检测 ,比较阴阳性种貂的生长状况和生产指标 ,阴性貂显著好于阳性貂 ,提出适合我国国情的针对阿留申病的水貂育种措施     

PEG诱导水分胁迫对柑桔幼苗细胞质膜透性及脯氨酸含量的影响

柑桔叶片相对含水量(RWC)随聚乙二醇(PEG)处理渗透势的降低而依次降低;叶片细胞质膜透性随PEG处理浓度的增加而依次增大;幼苗叶片累积脯氨酸的数量随水分胁迫时间和强度递增。     

PEG模拟水分胁迫对桑种萌发及抗性生理生化指标的影响

以“桂优62号”桑种为材料,在不同浓度高分子量的聚乙二醇（PEG6000）模拟的水分胁迫条件下,以摇床培养方法对其进行萌发培养。测定不同浓度PEG溶液（30％、25％、20％和双蒸水）中萌发种子的相关抗性生化指标,实验结果经统计分析表明,当PEG浓度为30％时,萌发种子中的脯氨酸、可溶性蛋白、可溶性糖和丙二醛的含量均极显著地高于CK（双蒸水）,说明在高浓度PEG溶液处理下能够萌发的种子具有较强的抗水分胁迫的能力,可以作为桑树抗旱育种的选择依据之一。     

模拟干旱对冷地型草坪草种子发芽的影响

采用蒸馏水及浓度为5%、10%、15%、20%的PEG溶液处理海特斯、多年生黑麦、草地早熟禾、午夜和维加斯种子,测定其发芽率,以研究PEG对种子萌发的影响.结果表明:不同浓度的PEG对冷地型草坪草种子的抑制作用不同,并且随PEG浓度的提高发芽率逐渐下降,不同品种草坪草对不同浓度PEG的适应性不同.分析可知,5种冷季型草坪草萌发期抗旱性由强到弱依次为:海特斯、维加斯、多年生黑麦、午夜和早熟禾.     

尼龙袋法评价香蕉叶单宁对瘤胃降解特性的影响

旨在探究香蕉叶单宁对奶牛瘤胃降解特性的影响和评价香蕉叶的饲喂价值。样品分别为苜蓿干草组(100%的苜蓿干草)、苜蓿干草和香蕉叶混合组(50%苜蓿干草+50%香蕉叶)、香蕉叶组(100%香蕉叶)、苜蓿干草组(100%的苜蓿干草)+6%聚乙二醇(6% PEG)、苜蓿干草和香蕉叶混合组(50%苜蓿干草+50%香蕉叶)+6%聚乙二醇(6% PEG)、香蕉叶组(100%香蕉叶)+6%聚乙二醇(6% PEG)。测定样品中的营养成分含量,使用3头安装了瘤胃瘘管的荷斯坦奶牛进行半体内试验,用尼龙袋法测定上述样品在瘤胃内0、2、4、8、12、24、48、72 h的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)的降解率,得到各营养物质的动态降解参数。结果表明,相较于苜蓿干草,香蕉叶干物质和粗蛋白的快速降解部分、慢速降解部分、慢速降解部分的降解速率、有效降解率是显著低于苜蓿干草的(P<0.001),香蕉叶和苜蓿的NDF的有效降解率没有显著差异。添加了PEG后,香蕉叶和香蕉叶苜蓿混合组的快速降解部分和有效降解率显著高于未添加PEG的两组(P<0.001)。结果表明香蕉叶中的单宁会抑制香蕉叶的瘤胃降解率,因此在使用香蕉叶调制反刍动物的饲料时需要控制单宁的含量。     

用PEG“引发”沙打旺种子活力及抗逆性的研究

应用ＰＥＧ“引发”沙打旺种子，能提高发芽率，增强发芽势，缩短发芽成苗时间。可以在北方半干旱地区充分有效地利用早春降雨。还可以提高幼苗的抗逆性，增强对早春寒的低卸能力，并探讨了ＰＥＧ渗透调控的机理和不同时间处理增强发芽势的效果。     

Procedure for PEG treatment of bovine sera used for cell culture in virus diagnosis

A method for PEG treatment of bovine sera to be used for cell cultures is presented. The procedure consists in repeated freezing and thawing cycles of the sera, after PEG precipitation until a clear solution is obtained. The sera, so prepared, are homogeneous in composition and appropriate for cell growth in primary cell cultures and cell lines.     

三种禾草萌发期抗旱性研究

采用蒸馏水及浓度为5%、10%、15%、20%和25%的PEG溶液处理梭罗草、青海草地早熟禾、垂穗披碱草种子,测定其发芽率、苗长、胚根/胚芽比值,以研究PEG对种子萌发的影响。结果显示:PEG浓度为5%时,青海草地早熟禾的相对发芽率出现小幅上升趋势,垂穗披碱草和梭罗草均呈下降趋势;不同处理对胚根/胚芽比值和苗长均有影响,梭罗草受影响小。因此这三种牧草在萌发期抗旱性依次为:梭罗草、青海草地早熟禾、垂穗披碱草。     

Toxicity nanoinsecticide based on clove essential oil against Tribolium castaneum (Herbst)

This study aimed to characterize nanoparticles loaded with clove (Syzygium aromaticum) essential oil-based polyethylene glycol (PEG) and to know their insecticidal activity against red flour beetle (Tribolium castaneum). The nanoparticles have irregular shapes in good dispersion. The nanoformulation could not enhance clove oil contact toxicity to T. castaneum, but could protect the oil from degradation and evaporation while simultaneously allowing sustained release, as indicated by the continued high toxicity for 16 weeks of storage.