簕杜鹃的扦插繁殖技术

介绍了我国南方地区广泛种植的园林观赏植物——簕杜鹃的扦插技术，就其基质、插穗准备和扦插方法及管理方面的技术进行阐述，旨在为生产上的大规模繁殖与开发利用提供技术支撑。     

Effect of lysophosphatidic acid on expression of integrin β6 in epithelial cells

AIM: To investigate the effect of lysophosphatidic acid (LPA) on the expression of integrin β₆ (ITGB6) for determining the role of transforming growth factor β (TGF-β) activation induced by LPA in this process. METHODS: Normal human bronchial epithelial (NHBE) cells were primarily cultured in 6 well plate and stimulated with LPA. The mRNA expression of ITGB6 and the level of cell surface ITGB6 protein were detected by RT-PCR and flow cytometry,respectively. The activity of active TGF-β induced by LPA was measured by the method of transformed mink lung epithelial cells (TMLC) transfected with TGF-β responsive plasminogen activator inhibitor 1(PAI-1) promoter fused with firefly luciferase reporter gene. RESULTS: After stimulated with LPA at concentration of 10 μmol/L for 2 h, the mRNA expression of ITGB6 in epithelial cells was significantly increased in a time-dependent manner. The results of flow cytometry showed that the protein level of ITGB6 on cell surface was obviously increased after treated with LPA at concentration of 10 μmol/L for 4 h. The active TGF-β induced by LPA in epithelial cells was blocked by an α_Vβ₆ blocking antibody. However, α_Vβ₆ blocking antibody failed to inhibit the mRNA expression of ITGB6 induced by LPA. CONCLUSION: LPA induces the mRNA and cell surface protein of ITGB6 in epithelial cells. The up-regulated ITGB6 expression by LPA is independent on LPA-induced TGF-β activation.     

Effects of immunoglobulin on circulating proinflammatory cytokines in children with hand-foot-mouth disease

AIM：To study the mechanism of intravenous administration of immunoglobulin for treatment of hand-foot-mouth disease. METHODS：Sixty-four children with hand-foot-mouth disease were randomly divided into treatment group and control group. The children in control group (n=32) were treated with ribavirin and given normal supportive therapy. The children in treatment group (n=32) were given ribavirin and normal supportive therapy plus intravenous administration of immunoglobulin (500 mg·kg-1·d-1) for 3 d. The levels of TNF-α, C-reactive protein (CRP) and IL-6 in venous blood were observed. RESULTS：The levels of TNF-α, CRP and IL-6 in treatment group and control group were all higher than those in the normal children. The levels of TNF-α, CRP and IL-6 in treatment group were similar to those in control group before treatment. The levels of TNF-α, CRP and IL-6 in treatment group were significantly lower than those in control group after treatment with immunoglobulin for 3 d. CONCLUSION：The systemic inflammatory response syndrome is one of the possible mechanisms of hand-foot-mouth disease. Intravenous administration of immunoglobulin decreases the proinflammatory cytokines in the patients with hand-foot-mouth disease.     

基于粒子图像测速仪的微小流道内固体颗粒运动规律的实验研究

为了解迷宫流道内固体颗粒的运动规律,提高灌水器的抗堵塞能力,采用粒子图像测速仪(particle image velocimetry,PIV)对不同直径和不同密度的固体颗粒在迷宫流道内的运动规律进行了实验观测。结果表明,根据颗粒的运动轨迹线可知齿形流道内水流在各齿尖做类似于正弦波的运动,靠近齿尖处形成主流区,靠近齿底处形成漩涡区;直径小(65mm)或密度低(1 740kg/m3)的颗粒跟随性比较好,颗粒直径或密度越大,运动越紊乱,出现的最小极值速度越低,越容易进入漩涡区并最终可能导致堵塞;与固体颗粒直径相比,颗粒密度对运动轨迹、路程和速度的影响较小。     

第19号台风“天兔”对汕头绿化树木破坏情况调查及应对措施

通过调查第19号台风“天兔”给汕头市区的绿化树木所造成的破坏,分析了台风对绿化树木造成破坏的原因,包括土壤软化、环境因子、地形因子、树种及病虫害等方面,并提出应对措施及建议。     

龙牙蕉组织培养技术

龙牙蕉吸芽茎尖经MS＋6-BA5．0mg／L＋AD20mg／L培养基诱导出不定芽后，在MS＋BA4mg／L＋NAA0．1mg／L培养基上继代培养对不定芽的分化较好，平均分化倍数为2．6倍以上，无根小苗在1／2MS＋NAA0．5mg／L＋AC1g／L培养基上进行生根培养，1周后长出新根。组培小苗移栽假植成活率94％，大田栽培表现正常。     

单克隆抗体-胶体金标记技术快速检测柑桔黄龙病

应用单克隆抗体一胶体金标记技术,采用徒手切片法和斑点试验法检测柑桔黄龙病获得成 功。该方法检测速度快,操作简单,成本低,适合柑桔种苗大批量检测和田间普查。采用单抗金标技术 对田间栽培的柑桔品种、柑桔实生苗、试管微芽嫁接苗、热处理脱毒后的嫁接苗进行检测,结果表明 田间栽培的6个柑桔品种不论是否显示黄龙病症状均检测到柑桔黄龙病病菌,而消毒种子播种所得 实生苗及试管微芽嫁接苗为无病株,带病株经过30d 40℃恒温热处理后产生的新梢及其嫁接苗绝大 部分也是无病株。     

萝卜种子发芽试验研究

分别采用不同浓度的赤霉素(GA3)、吲哚乙酸(IAA)、萘乙酸(NAA)处理萝卜种子,结果表明:经GA3100 mg/L,IAA100 mg/L,NAA0.5 mg/L处理可缩短发芽时间,提高发芽率、发芽势及活力指数.其中100 mg/L的GA3处理的效果最好.对萝卜幼苗早期生长都有良好的促进作用.     

球形棕囊藻的负趋光性和硝酸盐饥饿诱导鞭毛脱落试验

为了解释自然界中球形棕囊藻（Phaeocystis globosa）赤潮的生消现象,对其负趋光性和硝酸盐饥饿诱导鞭毛脱落进行试验。通过制作简易的实验装置,对球形棕囊藻生活史中的游动单细胞、不动单细胞和群体3种形态进行趋光性试验。结果显示,具有鞭毛的游动单细胞形态的球形棕囊藻负趋光性明显,其他2种形态则无负趋光性;当培养基中硝酸盐完全缺失时,或者用等量氮素的亚硝酸盐或尿素代替,球形棕囊藻细胞均不能正常生长,在硝酸盐饥饿状态下诱导游动单细胞鞭毛脱落成为不动单细胞,并逐渐聚集形成群体,不动单细胞和群体培养液添加硝酸盐继续培养后又出现大量游动单细胞。试验表明,球形棕囊藻具有负趋光性,硝酸盐饥饿能够诱导游动单细胞鞭毛脱落并逐渐形成群体,当硝酸盐营养得到补充时,游动单细胞即可再生,与其在自然界中的赤潮生消现象相吻合。     

基于ASP.NET的讲座预定系统的设计与实现

介绍了讲座预定系统的设计与实现及主要功能模块,包括用户注册与登录模块、最新讲座列表模块、讲座预定模块、座位查询模块、讲座调查模块、用户评论模块以及后台管理模块。