固氮芽孢杆菌的筛选及其生物学特性初探

采用无氮培养基富集、加热筛选固氮芽孢杆菌。筛选到了2株固氮芽孢杆菌,编号为JZ4和JZ5。采用固氮芽孢杆菌对唯一碳源、唯一氮源的利用和其对抗生素抗性的测定分析,探讨JZ4和JZ5对逆境的适应能力。试验结果显示:固氮芽孢杆菌JZ4、JZ5的竞争适应能力较强,而JZ5的竞争能力较JZ4强,可望进一步研发成为优良的固氮微生物肥料生产用菌种。     

合欢双条天牛初步研究

2004年对合欢双条天牛的生物学特性及其天敌进行了初步观察,并应用呋喃丹和久效磷进行了该虫防治试验。结果显示：呋喃丹柴油液涂干以及水溶液注射树干和树干基部木质部,效果均较理想,且无药害发生。表明呋喃丹防治合欢双条天牛效果较好。     

霍山石斛生产质量管理规范研究

以《中药材生产质量管理规范》(GAP)为指导原则,对安徽省地道中药材霍山石斛栽培的品种特性、生态环境、种苗标准、组培苗质量、组培苗驯化、栽培基质、施肥技术、采收与加工、包装、运输及档案等技术规程开展研究。研究并制定了霍山石斛生产质量管理规范。     

Preventive Effect of Monoammonium Glycyrrhizinate on the Injury Induced by Sodium Oleate in Hepatocytes of Cows in vitro

Hepatocytes injury is the main pathological feature of fatty liver disease in cows during the perinatal period. The aim of this study was to investigate the preventive effect of monoammonium glycyrrhizinate (MAG) on cell damage induced by sodium oleate in hepatocytes of cows. Primary hepatocytes of cows cultured in vitro were divided into 3 groups according to different treatments:control group (untreated primary hepatocytes) and 2 experimental groups (model group, MAG group:add 0 mg·L^-1, 0.25 mg·L^-1 MAG in culture media respectively, for 12 h and then use sodium oleate at a concentration of 0.25 mmol·L^-1 to induce hepatocytes steatosis), and then the activity of hepatocytes were detected by CCK-8, the cell apoptosis rate was calculated by DAPI fluorescence staining, the content of ALT and AST in the culture solution were detected by ultraviolet colorimetry, the levels of intracellular fat deposition were analyzed by oil red O staining and imageJ. Real-time fluorescence quantification (RT-qPCR) was used to detect the mRNA expression of lipid metabolism-related genes PPARα,SREBP-1c,ChREBP,CPT1,CPT2,MTP and inflammation-related genes TNF-α,NF-κB,IL-1β,IL-6,IL-8. The experimental results showed that hepatocytes preconditioning with 0.25 mg·L^-1 MAG could improve the activity of model cells (P<0.05), and inhibit the apoptosis rate of model cells effectively (P<0.05). The release levels of ALT and AST in the cell culture supernatant of the MAG group were lower than those of the model group (P<0.05). At the same time, compared with the model group, the number of intracellular lipid droplets and the average lipid droplet area of hepatocytes treated with MAG were significantly reduced (P<0.05). And then the levels of lipid metabolism genes ChREBP, PPARα, MTP and inflammation related genes TNF-α, IL-1β, NF-κB, IL-6, IL-8 mRNA were significantly down-regulated in MAG group than the model group (P<0.05). The results showed that MAG could alleviate the impact and relieve lipid deposition induced by sodium oleate in hepatocytes by inhibiting the expression of genes related to lipid metabolism and inflammatory cytokines, which indicated that it could provide protection for the liver.     

六安市油菜生产现状·存在问题及发展思考

2008~2013连续6年对六安市油菜主产县(区)采用实地访问的方式随机抽样90户,主要对油菜生产面积、生产管理、生产投入、生产效益、技术需求意愿及农户种油心理倾向等方面进行调查。通过调查,对当前油菜生产现状、存在问题及市场形势作了重点分析。针对存在的问题从政策支持、发展油菜"一菜多用"提高种植效益、推广轻简化生产新技术、加强健康食油宣传并提高食用菜籽油自给率等方面提出对策建议。     

复方霍山石斛含片清咽功能的动物试验研究

[目的]研究复方霍山石斛含片是否具有清咽功能。[方法]根据国食药监保化[2012]107号文件清咽功能动物试验要求,采用大鼠棉球植入、大鼠足趾肿胀、小鼠耳廓肿胀3种试验方法。具体将80只健康雄性大鼠、40只健康雄性小鼠按体重随机分2个大组,每个大组按低、中、高和空白对照4个剂量组[0.27、0.53、1.60、0 g/(kg·bw·d)],连续灌胃32 d后分别对不同剂量组进行棉球植入试验(肉芽肿的形成及测定)、足趾肿胀试验及测定、耳肿胀试验及测定。[结果]1.60 g/(kg·bw·d)组肉芽肿净量低于0 g/(kg·bw·d)组;0.53、1.60 g/(kg·bw·d)组1、2、4 h足趾肿胀率均低于0 g/(kg·bw·d)组,1.60 g/(kg·bw·d)组6 h足趾肿胀率低于0g/(kg·bw·d)组;1.60 g/(kg·bw·d)组耳廓肿胀率低于0 g/(kg·bw·d)组,差异均有统计学意义(P﹤0.05)。[结论]复方霍山石斛含片清咽功能的动物试验结果为阳性,具有清咽功能。     

山西某鸡棚改造育肥猪场腹泻病因诊断

为诊断山西省某鸡棚改猪场80~100日龄猪群发生腹泻病的病因。采集40份猪的肛拭子样品,通过实时荧光扩增和细菌分离鉴定的方法,进行实验室诊断。检测结果显示,腹泻样品中轮状病毒（Porcine rotavirus, PoRV）核酸100%为阳性,猪圆环病毒2型（Porcine circovirus type 2, PCV-2）核酸30%为阳性。猪流行性腹泻病毒(Porcine epidemic diarrhea, PED)、猪伪狂犬野毒(Pseudorabies virus, PrV)、猪瘟病毒(Swine fever virus, SFV)核酸检测均为阴性,大肠杆菌（Escherichia coli, E.coli）和沙门氏菌(Salmonella enteriditis, SE)100%呈阳性,仅对万古霉素和头孢曲松同时敏感。     

97份安徽省种植小麦品种HMW-GS组成及品质分析

为探究安徽小麦品种高分子量麦谷蛋白亚基（HMW-GS）组成及品质性状,对97份安徽省主要种植的小麦品种进行了HMW-GS组成及品质性状分析。结果表明,供试材料中共检测出10种HMW-GS亚基类型和19种亚基组合,在 Glu-A1位点检测出3种亚基类型,以1亚基（57.73%）和Null亚基 （40.21%）为主;在 Glu-B1位点共检测出3种亚基类型,以7+8亚基（52.58%）为主;在 Glu-D1位点共鉴定出4种亚基类型,优质亚基5+10占比最大,为45.36%;供试品种亚基组合品质得分在4~10分之间,10分的亚基组合1/7+8/5+10和1/17+18/5+10共19份材料（19.59%）,8分以下的共44份材料（45.36%）。对供试材料HMW-GS与品质性状进行相关分析发现, Glu-1的三个位点与硬度指数、溶剂保持力和吸水率相关达显著或极显著水平,对溶剂保持力影响表现为 Glu-A1> Glu-B1> Glu-D1;不同亚基组合对品质性状的影响存在差异,亚基组合为1/17+18/5+10的品种具有最高的硬度指数、4种溶剂保持力、吸水率、稳定时间和粉质质量指数,具有最低的L^*和W,该类品种有烟农19、泰农19、山农17和糯小麦1012;亚基组合为Null/7+9/4+12的品种具有最低的硬度指数、4种溶剂保持力、吸水率、稳定时间和粉质质量指数,具有最高的弱化度、L^*和W,该类品种有荃麦725、皖麦52和未来0818。     

安徽省稻米气候品质评价方法

为科学评价区域稻米气候品质提供技术支撑，该研究基于2008-2018年安徽省区域性试验稻米品质资料及对应站点气象数据，采用数理统计方法，明确了稻米品质形成关键期和最佳温度，建立了中籼和中粳稻米气候品质评价模型，并利用2018年分期播种试验稻米品质资料对模型进行了验证。结果表明：中籼和中粳稻米品质形成关键期分别为齐穗后33 d和36 d，稻米品质形成的适宜温度分别为24.8 ℃和23.0 ℃。将稻米气候品质划分为"特优""优""良好""一般"4个等级，对应中籼稻米气候品质指数（IACQ）范围分别为：IACQ≥3.40、3.09≤IACQ<3.40、2.73≤IACQ<3.09、IACQ<2.73，中粳稻米气候品质指数范围分别为：IACQ≥3.36、3.08≤IACQ<3.36、2.68≤IACQ<3.08、IACQ<2.68。经验证，与实际等级相比，模型计算得到的中籼和中粳稻米气候品质等级准确率均为80%。该研究建立的评价模型可用于中籼和中粳稻米的气候品质评价工作。