固氮芽孢杆菌的筛选及其生物学特性初探

采用无氮培养基富集、加热筛选固氮芽孢杆菌。筛选到了2株固氮芽孢杆菌,编号为JZ4和JZ5。采用固氮芽孢杆菌对唯一碳源、唯一氮源的利用和其对抗生素抗性的测定分析,探讨JZ4和JZ5对逆境的适应能力。试验结果显示:固氮芽孢杆菌JZ4、JZ5的竞争适应能力较强,而JZ5的竞争能力较JZ4强,可望进一步研发成为优良的固氮微生物肥料生产用菌种。

Isolation and Biological Characteristic Preliminary Investigation on Nitrogen- Fixing Bacillus

Nitrogen - free medium was used to isolate nitrogen - fixers and heat treatment was used for the nitrogen - fixing bacillus. Two strains termed as JZ4 and JZ5 were isolated. Investigate the ability of JZ4 and JZS＇ s resistance by the only carbon source utilization,the only source utilization and the situation of antibiotic resistance. Results showed： nitrogen -fix- ing bacillus JZ4 and JZ5 with high competition ability, but JZ5 was better than JZ4. So it is possible to be further developed as an excellent strain used in the microbial fertilizer production.