绵羊肌肉生长激素受体基因表达的发育性变化研究

[目的]探讨绵羊肌肉生长激素受体基因(GHR)在生长发育早期的表达。[方法]用real time PCR法对不同日龄雄性哈萨克羊和新疆细毛羊背最长肌GHRmRNA的表达进行定量分析。[结果]绵羊背最长肌GHRmRNA的表达随着日龄的增加先升后降,然后趋于水平,30日龄时最高。哈萨克羊的表达量在2~90日龄期间都极显著低于新疆细毛羊(P<0.01)。[结论]日龄和品种对绵羊肌肉GHR基因表达均有较大影响。     

绵羊肌肉生长激素受体基因表达的发育性变化研究

1材料与方法 1．1试验动物选取新疆维吾尔族自治区石河子市紫泥泉种羊场的2、30、60、90和120日龄的雄性哈萨克羊和新疆细毛羊各6只（120日龄的只有新疆细毛羊）,共计54只,测体重后屠宰,采取背最长肌,立即置于液氮中速冻,-70℃保存。     

绵羊肌肉生长激素受体基因表达的发育性变化研究(英文)

[Objective] The study aimed to explore the expression of muscular growth hormone receptor gene (GHR) in sheep at the early stage of growth and development. [Method] The GHR mRNA expression levels in longissimus dorsal muscles of male Kazak sheep and Xinjiang fine wool sheep with different ages were quantitatively analyzed by real time PCR. [Result] Sheep GHR mRNA expression level in longissimus dorsal muscle increased firstly followed by decline, and then kept steady until the end of the experiment, with the expression peak appearing on postnatal day 30. The GHR mRNA expression level of Kazak sheep was extremely lower than that of Xingjiang fine wool sheep from 2 to 90 days old (P<0.01). [Conclusion] Both age and breed had great effects on the expression of muscular GHR gene in sheep.     

浓香型白酒窖池古菌群落研究

采用PCR-SSCP技术研究了不同窖龄浓香型白酒窖池古菌群落的变化规律,结果发现:①所有窖泥样品均出现7～12条较清晰的条带,其中第19,26号条带在所有样品中均有检出,且优势度较高;②随着窖龄的增加,窖池不同位置样品的第19号条带优势度基本上都呈先增加后降低的趋势,第26号条带优势度基本上都呈先降低后增加的趋势;③所有窖泥样品平均生物多样性指数都在1.78～2.26之间,随着窖龄的增加,窖壁上层与窖底样品的多样性指数呈增加趋势,窖壁下层样品呈先降低后增加的趋势;④不同窖龄窖池SSCP图谱相似性指数较低,在0.17～0.43之间,而同一窖池不同位置样品的相似性指数较高,在0.42～0.95之间.     

绵羊肌肉IGF-Ⅰ基因表达的发育性变化研究

[Objective] To detect the mRNA of muscle insulin-like growth factorⅠ (IGF-Ⅰ) in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [Method] With real-time quantitative PCR, the muscle IGF-Ⅰ mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [Result] The IGF-Ⅰ mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-Ⅰ expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old (P>0.05), but was lower than that of the latter with extremely significant difference (P<0.01) from 30 to 60 days old. [Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-Ⅰ mRNA, but the expression level is different between these two species.     

浓香型大曲微生物群落代谢多样性研究

试验利用BIOLOG ECO微平板分析了浓香型大曲发酵过程中微生物群落代谢特性.结果表明:发酵前40 d,浓香型大曲微生物代谢活性先上升后下降,可能是温度和水分共同作用的结果;微生物群落的功能多样性变化不明显.

Abstract：

The BIOLOG ECO Microplates were used to analyze the metabolism of the microflora in LuzhouFlavor Daqu at various stages in the fermenting process. The results showed that in the first 40 days of fermentation, the metabolic activity of the microorganisms of Luzhou-Flavor Daqu increased initially and decreased afterwards, probably resulting from the combined function of temperature and moisture content.The change in microbial community functional diversity was not obvious.     

自身免疫性胰腺炎6例临床观察

探讨自身免疫性胰腺炎的临床特点和治疗效果。选择海口市人民医院2008年9月至2011年9月自身免疫学胰腺炎患者6例，分析患者临床表现，对患者进行实验室、影像学、病理组织学检测，了解本病上述检查结果特点，并给以相关治疗。结果表明：6例患者中，5例患者给予糖皮质激素类药物治疗，1例合并感染未给予糖皮质激素类药物治疗，而给予对症处理；5例激素治疗患者中，4例患者疗效显著，临床症状改善，另外1例患者激素治疗效果差而行胆总管支架置入，术后患者黄疸症状缓解。此类胰腺炎对糖皮质激素类药物敏感，预后较好。     

冬麦灌水次数对产量的影响

试验结果表明，冬小麦以灌底墒水、冬水、返青水、拔节水、抽穗水和灌浆水6水产量最高，其单株性状和产量构成因素最为协调。     

冬麦灌水时期对产量的影响

通过试验表明，冬小麦返青期至拔节期间灌水以4月21日以前效果好，其单株性状及产量要素表现好，增产明显。     

五角枫翅果中单宁的提取及其改性研究

研究五角枫翅果中单宁的提取和改性方法,以制备新型水处理剂。采用丙酮-水的混合液提取五角枫翅果中单宁,并找出最优方案,得到较高的提出率;进而用甲醛、二甲胺等试剂对该单宁提出物进行了阳离子改性。结果表明,用丙酮–水的混合液提取出五角枫翅果中单宁的提出率较高,其阳离子化产物(简称为PIT)对污水的絮凝效果良好、对工业废水带来的结垢、腐蚀等负面效应均有一定的抑制作用,具有较好的应用前景。