新疆巴州地区猪弓形虫病的血清学检测

为了解新疆巴州部分地区猪感染弓形虫病的现状,本试验以弓形虫Tg SAG2蛋白作为包被抗原对巴州地区433份猪血清样品进行了ELISA检测。结果显示,平均阳性率为12.70%(55/433)。其中,散养猪场阳性率为16.83%(35/208),规模化养殖场为9.94%(16/161),散养猪场阳性检出率显著高于规模化养殖场(P0.05);在各县市采集的血清样品中,尉犁县阳性率最高,为26.53%(13/49),和静县和焉耆县阳性率最低,分别为6.52%(3/46)和6.82%(3/44)。本次试验大范围地调查了巴州地区猪弓形虫病的流行现状,为该地区弓形虫病的防控奠定了理论依据。     

青海都兰野生反刍兽和啮齿动物布鲁菌病血清学检查

采集青海省都兰县境内动物血清:野生马鹿15份,野生岩羊6份,野生黄羊血清20份,高原鼠兔100份,喜马拉雅旱獭20份进行布鲁菌病检测,结果:被检野生马鹿血清15份,试管凝集阳性反应的2份,占13.3%,虎红凝集试验阳性反应1份,占6%;被检野生岩羊6份,试管凝集阳性反应1份,占16.7%,虎红凝集试验为阴性反应;被检野生黄羊血清20份,试管凝集阳性反应的3份,占15%,虎红凝集试验阳性反应1份,占5%;被检高原鼠兔血清100份,试管凝集阳性反应的3份,占3%,虎红凝集试验有阳性反应1份,占1%;被检喜马拉雅旱獭20只,试管凝集阳性反应的1只,占5%,虎红凝集试验为阴性反应。     

Research Progress on Rapid Serological Assays for Diagnosis of Japanese Encephalitis B

Japanese encephalitis B (JE) is a zoonosis caused by Japanese encephalitis virus which invades the central nervous system,infected by mosquito.Various animals could be the sower and reservoir of JE including swine,horse,cattle and avian.JE could cause a major public health trouble and a horrible crisis of the farm all in Asia and the Western Pacific region.Therefore,it is necessary to diagnose it rapidly and accurately.This review article made a sketch of approaches for the rapid serological assays of JE,including traditional method,emerging technologies and specific improvements of the assay,so as to provide references for the study of rapid,simple and accurate JE diagnose methods.     

麻雀新城疫和传染性法氏囊病血清学调查和病原分离

分别采用HA-HI试验和AGID试验对96份麻雀的血清进行了新城疫和传染性法氏囊病的抗体检测,同时,又对这些麻雀的内脏进行了病毒的分离培养和鉴定。结果显示,血清抗体为阴性,病毒的分离鉴定也为阴性。     

新疆东疆部分农区羊衣原体病的血清学调查

鹦鹉热衣原体（Chlamydia psittaci）是一种可在多种动物间广泛传播的人畜共患传染病病原，它可引起不同种动物的多症侯群疾病。绵羊感染后可引起流产、早产、死产及肺炎、多发性关节炎、脑脊髓炎和腹泻，种公羊发生睾丸炎等病症。自1949年首次在苏格兰报道了该病的发生，随后世界各地有20多个国家相继报道了该病的发生，在青海省藏系羊也确诊有该病的发生。     

布氏杆菌病鉴别诊断技术以及疫苗研究进展

<正>布氏杆菌病(Brucellosis)是由布氏杆菌属引起的全世界范围流行的人畜共患病。布氏杆菌可感染多种家畜和野生动物,同时也可感染人,特别是羊布氏杆菌对人的威胁最大,引起相似的临床症状和病理损伤,如睾丸炎或附睾炎、不育、生殖器官及胎膜发炎、流产、不孕、关节炎、气管炎及各种组织的局部病灶等,导致巨大的经济损失和严重的公共卫生问题。在诊断方法上目前主要通过微生物学和血清学方法进行,诊断结果不是很准确,主要是     

鸡包涵体肝炎及其诊治

鸡包涵体肝炎是由禽腺病毒引起的鸡的一种急性传染病，以病鸡死亡突然增多，严重贫血、黄疸、肝脏重大出血和坏死灶，可见肝细胞核内有包涵体。1984年以后，我国通过血清学检查确定东北、华北、华南各地均有该病存在，山东省也不例外。有人从内蒙古某地分离到一株IBH病毒，经鉴定为禽腺病毒8型。     

兔水疱性口炎的血清学诊断

1液相阻断酶联免疫吸附实验 样品采集为被检兔的血清样品。固相载体用pH值9.6的碳酸盐（CBS）缓冲液将兔抗血清作适当稀释,包被ELISA反应板,4℃过夜。然后用含0.05%吐温-20的磷酸盐缓冲液（PBST）洗板3次,再用2%卵白蛋白室温封闭1小时。洗板3次后备用。     

上海城区宠物犬犬流感的血清学调查

为了解上海城区宠物犬犬流感的感染情况,作者于2013年4月至2013年9月收集了上海城区20家宠物医院就诊犬共460份血清样本,通过ELISA方法检测对犬流感进行了血清学调查。结果表明:460份血清样品中抗体阳性率为14.57%,母犬犬流感血清抗体阳性率略高于公犬。     

Bluetongue Virus （BTV） Serological Survey and Evidence of Emergent BTV-8 Serotype in Morocco

Bluetongue （BT） is an infectious, non-contagious arthropod-borne disease that infects all ruminants, including sheep, cattle, deer, goats and camelids. Bluetongue virus （BTV） belonged to Reoviridae is ARN genome of 19 Kb. Twenty-six BTV serotypes have long been recognized, to be associated with severe disease in certain breeds of sheep, whereas cattle and goats are usually sub-clinically affected. Before 2004, BT was considered an exotic disease in Morocco, however, the first outbreak was observed in 2004 in sheep. This outbreak was caused by the isolated BTV-4. Two years later a BTV-1 emerged in Morocco. Both serotype 1 and serotype 4 circulated after 2007 across the country. The aims of the present work was to perform a serological study on sheep from different regions in Morocco in order to clarify the current BTV epidemiological situation and its evolution from 2009 to 2012, to determine the co-infection rate, and to confirm the possible circulation of other BTV Serotype mainly the BTV-8. All of 436 sera were tested by serum neutralization using reference strains. Results confirm the presence of BTV-4, BTV-1 and BTV-8. However, the present study report for the first time the emerging BTV-8 circulation in Morocco. Moreover, the founding reveal as well a higher co-infection rate in cattle compared to sheep.