Determination of the Tissue Distribution of PPO on Caramote Prawn (Melicertus kerathurus,Forskal, 1755) and Jinga Shrimp (Metapenaeus affinis,H. Milne Edwards, 1837) Caught from Izmir

Tissue distribution of polyphenol oxidase (PPO) was studied in caramote prawn (Melicertus kerathurus) and jinga shrimp (Metapenaeus affinis) postmortem. Activity was analyzed in different tissues: the head (cephalothorax + carapace), carapace, exoskeleton of the abdomen, pereopods, and maxillipeds, individually. Based on the results, PPO activity of jinga shrimp was determined to be higher than caramote prawn during the research period. Partial tissue distribution showed that the highest activity was determined in carapace by using 70% ammonium sulfate fractions. Partial tissue distribution was also used to determine the spread of black spots. In addition, comparison between the PPO levels of caramote prawn and jinga shrimp was performed. The importance of these species is their economic value. However, there is no available data in the literature about caramote prawn and jinga shrimp. The determined data showed us that using antioxidant chemicals would be suitable for jinga shrimp.     

Vitamin D status in cattle with malignant catarrhal fever

The aim of the present study was to determine the vitamin D status in cattle with malignant catarrhal fever (MCF). Twelve cattle diagnosed as MCF and 6 healthy cattle (controls) were used in the study. Serum 1,25-dihydroxyvitamin D(3) (1,25-D), 25-hydroxyvitamin D(3) (25-D), calcium, phosphorus and parathyroid hormone (PTH) levels were determined as 96.83 pg/ml, 30.0 ng/ml, 2.19 mmol/l, 1.57 mmol/l and 15.21 pg/ml in MCF group and 42.33 pg/ml, 37.0 ng/ml, 2.43 mmol/l, 1.96 mmol/l and 36.08 pg/ml in controls, respectively. Although serum 1,25-D level in the MCF group was increased (P<0.01), serum calcium (P<0.01) and PTH (P<0.05) levels were decreased compared to the controls. The results suggest that there might be an interaction between vitamin D status and MCF.     

Multivariate Statistical Techniques for the Assessment of Surface Water Quality at the Mid-Black Sea Coast of Turkey

The aim of this study was to investigate the seasonal and spatial variations in surface water quality at the mid-Black Sea coast of Turkey. The samples were collected from ten monitoring stations including rivers and sea water during the years from 2007 to 2008. The samples were analyzed for 25 parameters: total carbon, total inorganic carbon, total organic carbon, chromium, cadmium, copper, lead, iron, nickel, manganese, phenol, surfactants, ammonium, nitrite and nitrate-nitrogen, total phosphorus, adsorbable organic halogen, sulfate, hardness, dissolved oxygen, pH, temperature, total dissolved solids, electrical conductivity, and salinity. Multivariate statistical techniques, cluster analysis (CA) and factor analysis/principal component analysis (FA/PCA), were applied to analyze the similarities among the sampling sites to identify the source apportionment of pollution parameters in surface waters. The results indicate that seven factors for river water explained 82.24% of the variance. In seawater, seven factors account for 89.65% of the total variance. Varifactors obtained from factor analysis indicate that the parameters responsible for water quality variations are mainly related to organic pollution (municipal effluents), inorganic pollution (industrial effluents and waste disposal areas), nutrients (agricultural runoff), and dissolved salts (soil leaching and runoff process).     

The expressions of HSP70 and ��B-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs

This study describes the expression of heat shock protein70 (HSP70) and alpha-basic-crystallin (α-BC) and their association with apoptosis and some related adaptor proteins in the pathogenesis of foot-and-mouth disease virus (FMDV)-induced myocarditis in lambs. HSP70 was generally overexpressed in the myocardial tissues and inflammatory cells of FMDV-induced myocarditis with differential accumulation and localization in same hearts when compared to non-foot-and-mouth disease control hearts. α-BC immunolabeling showed coarse aggregations in the Z line of the cardiomyocytes in FMDV-infected hearts in contrast to control hearts. Overall, the results of this study show that the anti-apoptotic proteins, HSP70 and α-BC, were overexpressed with increased apoptosis in FMDV-infected heart tissues. Both proteins failed to protect the cardiomyocytes from apoptosis as defense mechanisms to the FMDV during the infection, suggesting that the virus is able to increase apoptosis via both downregulation and/or upregulation of these anti-apoptotic proteins.     

SO2 Levels at Forested Mountains around Izmir,Turkey and their Possible Sources

Daily average SO₂ concentrations were measured during August 1999–September 2000 period in the mountains around Izmir. Sampling devices to collect integrated daily SO₂ samples were located at four different sites. These sites were Yamanlar Mountain in the north, Tekketepe height of Karabelen Mountain in the south, and two mountain villages (Kiziluzum and Bespinar) located east of Izmir. Samples were analyzed by using standard methods. Maximum daily SO₂ concentrations up to 433 μg m^-3 were found with the average values ranging between 75–135 μg m^-3 per day at the four mountain stations. Annual mean values were above the threshold levels acceptable for the health of trees. It is concluded that such high SO₂ pollution might have caused the noted decline in the forests.In order to decide the sources of such high levels of pollution on the forested mountains, trajectory analyses were carried out. Results obtained at Tekketepe station are given in this article and they point to the fractional contributions of dense industrial areas around Izmir, to the forested heights of Tekketepe. Local topography and location of industrial zones around the city are seen to have a strong effect on the deteriorated air quality over the mountains. The annual and seasonal averages and ten maximum daily measurements at the Tekketepe sampling site indicate that the highest contribution to the deterioration of air quality is associated with the northerly wind sectors.     

Immunohistochemical distribution of alpha B-crystallin in the cerebellum of dogs infected with canine distemper virus

The cerebella of 12 dogs infected with canine distemper virus (CDV) and those of three normal dogs were examined. The avidin-biotin-peroxidase complex technique was used to detect alphaB-crystallin (alphaB-c) immunoreactivity and immunolocalisation of the CDV antigen. CDV antigens, immunopositive astrocytes, oligodendrocytes and granular neurons were seen in both the white and grey matter of the infected dogs. In the controls, alphaB-c immunopositive glial cells were seen in the white matter and around the Purkinje cells. In dogs with distemper, alphaB-c immunoreactivity was not observed in some of the glial cells around the Purkinje cells. A significant negative correlation of P < 0.01 level was found between areas of severe demyelination and the number of alphaB-c immunopositive cells in dogs infected with CDV. Such correlation was not observed between mild and moderate demyelinating areas and alphaB-c immunostaining. The alphaB-crystallin/ total number of cells ratio was found to be significant in severely affected demyelinating areas (P < 0.05). These data indicate that there was a relationship between the degrees of CDV associated with demyelination and the level of alphaB-c expression in the glial cells.     

Tarantula cubensis extract alters the degree of apoptosis and mitosis in canine mammary adenocarcinomas

In the present study, 13 clinical cases of canine mammary adenocarcinoma were evaluated in order to understand the effect of Tarantula cubensis extract (TCE) on tumor tissue. Punch biopsies were taken from the tumors before treatment with TCE. Subcutaneous injections of TCE were administered three times at weekly intervals (3 mL per dog). Between days 7 and 10 after the third injection, the tumor masses were extirpated by complete unilateral mastectomy. Pre- and post-treatment tumor tissues were immunohistochemically assessed. The expression of B-cell lymphoma 2 (Bcl-2) was found to be higher in pre-treatment compared to post-treatment tissues (p < 0.01) whereas Ki-67 expression was lower in post-treatment tissues (p < 0.01). No significant differences in fibroblast growth factor or vascular endothelial growth factor expression were observed between pre- and post-treatment tissues (p > 0.05). The apoptotic index was determined to be low before treatment and increased during treatment. These results suggest that TCE may be effective for controlling the local growth of canine mammary adenocarcinoma by regulating apoptosis.     

Effect of different plant oils on growth performance,fatty acid composition and flesh quality of rainbow trout (Oncorhynchus mykiss)

This experiment intended to assess the effect of sesame (SO), sunflower (SFO) and linseed (LO) oils on growth performance, fatty acid composition of fillet and liver or flesh quality traits of rainbow trout. Fish fed different four iso‐nitrogenous and iso‐lipidic experimental feeds. The control feed contained only fish oil as the primary lipid source. The fillet eicosapentaenoic acid and docosahexaenoic acid levels were the highest in fish fed control feed. In contrast, the liver eicosapentaenoic acid level was the highest in fish fed LO feed. Fish fed SFO feed had the highest level of total n?6 fatty acids in fillet and liver. Fish fed SO feed had the highest level of 18:1 n?9 fatty acid in fillet and liver. During the 12 days refrigerated storage period at 1°C, thiobarbituric acid (TBA) and pH values gradually increased in all dietary groups. However, trimethylamin nitrogen (TMA‐N) values increased in all dietary groups between days 0 and 9 during the storage period. Generally, pH value in fillets of control group was slightly higher than the other fish groups during 12 days refrigerated storage. Nevertheless, the chemical indicators of spoilage, TBA, TMA‐N and pH values were in the limit of acceptability for human consumption. Results of growth performance and chemical tests in the present study showed that sesame, linseed and sunflower oils could be used in feeds for rainbow trout. Moreover, we concluded that further researches should be carried out on the partial replacement of fish oil by sesame oil in rainbow trout nutrition.