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11.
Kazuaki TANAKA Tatsuya TAKIZAWA Tashi DORJI Takashi AMANO Hideyuki MANNEN Yoshizane MAEDA Yoshio YAMAMOTO Takao NAMIKAWA 《Animal Science Journal》2011,82(1):36-45
The electrophoretic variation in bovine hemoglobin‐beta (HBB) is one of the most investigated genetic markers. The presence of a unique HBB variant, HBBX, in Southeast Asian cattle has been reputed as a sign of gene‐flow from wild bovine species. In this study, we analyzed the DNA sequences of HBB genes in domestic and wild bovine species to verify this belief. Isoelectric focusing of HBB chain revealed that the HBBX in domestic cattle had dimorphism and was separated into HBBX1 and HBBX2. The HBBX1 had the same DNA sequence of the common HBB variant in gayal (Bos gaurus frontalis), while some of the HBBX2 were identical with that of Cambodian banteng (Bos javanicus birmanicus). As a result, we confirmed that the bovine HBB variants can be a good indicator of introgression between wild and domestic cattle. The HBBX1 was always predominant to HBBX2 in the continental populations, suggesting that the gaur had contributed to the gene pool of domestic cattle in this region much more than the banteng. On the other hand, the mitochondrial DNA analysis could not detect gene‐flow from wild species. Autosomal markers that can trace the phylogeny between alleles are suitable for the assessment of bovine interspecific introgression. 相似文献
12.
Kazuki OKADA Shinjiro AMANO Yoshio KAWAMURA Yumiko KAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1311-1313
An 8-year-old, spayed, female Shiba dog was presented to a referring veterinarian with a
complaint of chronic diarrhea and anorexia. Ultrasound and radiographs revealed an
irregular mass in the pelvic cavity. The mass and the affected section of colon were
surgically removed. Histopathological examination revealed multifocal coalescing
granulomas and effaced intestinal structures. Central necrotic debris surrounded by
multinucleated giant cells, lymphocytes, plasma cells and neutrophils was observed.
Numerous, irregularly branched hyphae with pale basophilic, thin walls and occasional
bulbous enlargements at the tips were present. Polymerase chain reaction identified
Basidiobolus ranarum, successfully confirming a definitive diagnosis of
basidiobolomycosis. To the best of our knowledge, this is the first report of intestinal
basidiobolomycosis in a dog. 相似文献
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14.
Michinari YOKOHAMA Takeshi MASUDA Takashi AMANO Hiroki HIRAYAMA Takashi MANABE 《Animal Science Journal》2004,75(5):401-405
As an analysis of the cashmere proteins by Type IV 2‐DE, ten kinds of components, including three components with molecular mass 42–50 kDa whose expression level increased in the winter, were separated. In analyzing nine components of these ten using a mass spectrometer, the three components of molecular mass 70–120 kDa and pI 5.3 were identified as keratin type II microfibrillar (accession no. KRSHL2 ), keratin 48 k type I microfibrillar component 8c‐1 (accession no. KRSHL1 ) and cytosolic phospholipase A2 (accession no. O77793 ), respectively. The three components whose expression level increased in the winter, were identified as keratin type I microfibrillar 48 kDa component 8C‐1 (accession no. P02534 ) and keratin type I microfibrillar 47.6 kDa (accession no. P25690 ) (pI 5.2/42 kDa), keratin type II microfibrillar component 7C (accession no. P15241 ) and keratin typeII‐sheep (accession no. S34165 ) (pI 5.5/45 kDa), and the keratin type II microfibrillar component 5 (accession no. P25691 ) (pI 5.8–6.0/45 kDa), respectively. The three components of less than 17 kDa were identified as hair keratin type II intermediate filament (accession no. CAA51836 ) (pI 5.2) and keratin high‐sulfur matrix protein IIIB2 (accession no. P02447 ) with a different isoelectric point (pI 5.4 and 5.9), respectively. 相似文献
15.
Yuka MORITA Naoshi HIRAMATSU Toshiaki FUJITA Haruna AMANO Etsuko KATSUMATA Kazutoshi ARAI Toshihide IWASAKI Takashi TODO Akihiko HARA 《The Journal of reproduction and development》2013,59(4):368-377
A single radial immunodiffusion (SRID) assay and a chemiluminescent immunoassay (CLIA)
were initially developed for alpha-fetoprotein (AFP) of the striped dolphin. Utilizing
these developed assays, we investigated pregnancy-associated changes in the levels of AFP
in the sera of fetuses and pregnant females of three dolphin species; samples were either
collected from captive individuals or obtained as fishery by-products. The concentrations
of AFP in the fetal serum ranged from 419.0 to 2026.3 μg/ml in the striped dolphin, 12.6
to 1218.7 μg/ml (for an AFP equivalent; eqAFP) in the common bottlenose dolphin and 770.6
to 3129.1 μg eqAFP/ml in the Risso''s dolphin. AFP levels decreased with increased fetal
size in fetuses over 20 cm in length. The concentrations of AFP in sera of pregnant
females ranged from 7.18 to 8068.7 ng/ml in the striped dolphin, 6.6 to 1241.1 ng eqAFP/ml
in the common bottlenose dolphin and 3.4 to 2868.7 ng eqAFP/ml in the Risso''s dolphin. The
levels in most pregnant females were equal to or lower than those found in males and
nonpregnant individuals, although a few pregnant females exhibited extremely high levels
(in the range of hundreds to thousands of nanograms per milliliter). Such high levels of
AFP were not observed during pseudopregnancy. To our knowledge, this is the first report
on basal profiles for serum AFP levels in small odontocetes. The profiles indicated that
AFP may play a significant role during embryonic development, although maternal levels do
not appear to be a diagnostic biomarker for monitoring pregnancy. 相似文献