微波消解-电感耦合等离子体质谱法同时测定普洱茶中9种金属元素含量

研究建立了普洱茶中9种金属元素含量的测定方法,样品经微波消解后,采用分子量相近原则选择45Sc、73Ge、115In、209Bi之一作为内标,用电感耦合等离子体质谱法(ICP-MS)同时测定普洱茶中铅、砷、镉、铬、锰、铜、锌、镍、硒的含量。检出限为0.0034~0.3100 mg/kg;在1.0~50.0 ng/mL线性范围内,相关系数为0.9991~1.0000;0.5、2.0、4.0 mg/kg 3个加标质量分数的平均回收率均在90.3%~107.9%之间,RSD均小于5%。该方法基质干扰小、灵敏度高、重复性好、定性和定量准确可靠,能满足普洱茶中多种金属元素的测定需求,可用于普洱茶的质量控制和安全评价。     

东台市工厂化育苗产业的发展现状及建议

东台地区顺应市场农业现代化发展需求，支持和推进瓜果蔬菜育苗产业化，已形成一定规模和技术体系，但也存在企业运营水平差异、缺乏专业技术培训、产业经营淡旺不均等问题；因此，要探讨加快工厂化育苗产业健康发展的途径和思路，尤其要构建质量与风险管控机制、创建个性化品牌、提升售后服务水平，旨在促进瓜果蔬菜育苗产业化经营的标准化、现代化。     

浅谈大兴区社区蔬菜技术服务

为提升社区居民的生活满意度,进一步探索阳台蔬菜管理技术、种植品种和管理模式,大兴区蔬菜技术推广站开展了基层调研、专业方案设计、观摩培训等社区蔬菜技术服务工作,并取得了较好的效果:服务范围不断扩大、栽培设备及配套技术逐渐完善、社区氛围良好等;并对发展前景进行了展望:未来将会向省空间、智能化、低碳化的多功能蔬菜种植模式发展。     

灰霉菌侵染对番茄幼苗活性氧积累及抗氧化酶活性的影响

为进一步分析活性氧和抗氧化防御系统在番茄抗病信号调控机制中的作用,为提高产量和品质提供科学依据,以4叶1心的番茄幼苗为试验材料,比较番茄幼苗受灰霉菌侵染后叶片O_2~-·以及抗氧化酶、内源褪黑素等的变化。结果表明:番茄幼苗接种灰霉菌后,随着时间的延长,幼苗叶片上病斑斑点逐渐增多,灰霉菌含量显著增多,叶色逐渐变浅,植株表现明显的发病症状;灰霉病菌侵染后番茄体内O_2~-·活性爆发,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降,过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性上升,褪黑素含量增加;上述变化都是番茄幼苗为了抵御灰霉菌的生物胁迫而发生的应激反应。综上,植物在遭受病原菌等生物胁迫时不能简单地推断SOD、CAT和POD等抗氧化酶活性一定升高,植物抵御病原菌侵染更多地依赖于抗性信号路径中信号物质的激活和积累。     

不同播期对黄秋葵长势、抗病性和产量的影响

为在河北省推广种植黄秋葵,在安国、保定、邯郸、衡水和石家庄5个试验点进行黄秋葵分期播种试验,研究了不同播期对石秋葵1号、红玉和五福3个品种生长势、抗病性和产量的影响。结果表明:播期对石秋葵1号、红玉和五福生长势、抗病性和产量具有较大的影响,随着播期的推迟,3个品种的茎粗大致呈逐渐增高的趋势,株高呈先降后升的趋势(除2018年的红玉外),单株结果数则先增加后减少,单果质量(除五福外)逐渐增大;以4月20日、25日和30日为播期,得到的黄秋葵单株产量和折合667 m~2产量较高。综合得出,河北省黄秋葵种植以4月20日、25日、30日为播期最为适宜。     

施用多功能生态肥对番茄生长和经济效益的影响

为了解决敦煌市蔬菜品质差、产量低而不稳的疑难问题,为温室蔬菜安全生产提供技术支撑,进行了多功能生态肥对番茄生长和效益的研究。结果表明:多功能生态肥原料间的主次效应(R)是:B(番茄专用肥,R=26.92)A(有机生态肥,R=24.29)C(土壤消毒杀菌剂,R=12.55);最佳配方组合是:m(有机生态肥)∶m(番茄专用肥)∶m(土壤消毒杀菌剂)=0.818 6∶0.177 4∶0.004 0。施用多功能生态肥与施用传统化肥比较,番茄早疫病发病率和可滴定酸分别降低66.67%和28.00%;株高、生长速度、茎粗、地上部分鲜质量和地上部分干质量分别增加4.45%、3.81%、7.61%、8.40%和6.67%;单果质量、单株果质量、产量、可溶性糖和VC含量分别增加6.23%、9.26%、8.82%、27.46%和23.65%;施肥利润和肥料投资效率分别增加1.84×10~4元/hm~2和1.23元/元。在甘肃省敦煌市肃州镇的温室土壤上施用多功能生态肥,改善了番茄品质,提高了番茄的经济效益。     

苜蓿青贮销售的“痛点”与突破

苜蓿蛋白质含量高,消化率高,营养价值好。随着畜牧业的发展和农业结构的调整,我国苜蓿种植面积逐年扩大。由于收获期雨热同季导致割晒苜蓿自然晒干较为困难,所以制作成青贮成为近几年苜蓿高效利用的重要方法之一。苜蓿青贮具有适口性好、易于保存、加工损耗低等优点,国内苜蓿种植户纷纷加入制作苜蓿青贮的队伍,但并未推动该产业快速、良性发展。本文探讨苜蓿青贮销售的痛点和难点,并提出建议,以期为同行提供参考。     

Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines

The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines.     

Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5

Cadmium (Cd) intake is harmful to human health and Cd contamination in rice grains represents a severe threat to those consuming rice as a staple food. Knockout of Cd transporters is a promising strategy to reduce Cd accumulation in rice grains. OsNRAMP5 is the major transporter for Cd and manganese (Mn) uptake in rice. Nevertheless, it is uncertain whether knockout of OsNRAMP5 is applicable to produce low Cd rice without affecting plant growth and grain yield. In this study, we adopted CRISPR/Cas9-based gene editing technology to knock out OsNRAMP5 in two japonica varieties. We generated three independent transgene-free osnramp5 mutants and investigated the effect of osnramp5 mutations on Cd accumulation and plant growth. Hydroponic experiments showed that plant growth and chlorophyll content were significantly reduced in osnramp5 mutants at low Mn conditions, and this defective growth in the mutants could be fully rescued by supply of high levels of Mn. Cd and Mn accumulation in both roots and shoots was markedly reduced in the mutants compared to that in wild-type plants. In paddy field experiments, although Cd in flag leaves and grains was greatly reduced in osnramp5 mutants, some agronomic traits including plant height, seed setting rate, and grain number per panicle were affected in the mutants, which ultimately caused a mild reduction in grain yield. The reduced plant growth in the mutants can be attributed to a marked decrease in Mn accumulation. Our results reveal that the manipulation of OsNRAMP5 should be treated with caution: When assessing the applicability of osnramp5 mutants, soil pH and soil water content in paddy fields need to be taken into consideration, since they might affect the levels of available Mn in the soil and consequently determine the effect of the mutation on grain yield.