右美托咪定抑制NOX4缓解急性应激致大鼠肾损伤

本研究拟探索右美托咪定（dexmedetomidine,DEX）对大鼠急性应激致肾损伤的保护作用,并从氧化应激的角度探索DEX对大鼠肾的保护通路。本研究使用了急性束缚应激模型,其中,大鼠被迫游泳15 min,并束缚3 h。本试验采用生化检测、组织病理学切片观察以评估肾功能,然后测定了氧化应激以及氧化应激的相关通路蛋白。旷场试验证实成功建立了急性应激模型。急性应激引起的肾损伤增加了NOX4,降低了Nrf2/HO-1/NQO1表达水平。DEX可降低NOX4表达,同时升高Nrf2/HO-1/NQO1的表达水平。DEX治疗组与急性应激组相比的肾生化结果明显恢复正常,病理切片观察损伤显著降低。试验结果表明,DEX治疗急性应激可影响NOX4/Nrf2/HO-1/NQO1信号通路,并抑制氧化应激。因此,DEX对急性应激引起的肾损伤具有保护作用,并在应激综合征中具有潜在的临床应用。     

对汽车撞柱的仿真研究

由于许多汽车事故是因为车辆撞枉而造成的，根据采集得到的真实事故数据，应用LS—DYNA和MADY—MO对车辆撞柱进行仿真，时仿真结果与事故调查结果比较分析表明，该仿真结果能真实反应实际撞柱情况，仿真方法的有效性得以证实。     

Effects of naringenin on PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways in rats with myocardial ischemia/reperfusion injury

AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion （I/R） rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats （n=48） were randomly divided into sham operation （sham） group, model （I/R） group, naringenin treatment （NAR） group and naringenin+LY294002 （NL） group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I （cTnI） was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 （GRP78）, C/EBP homologous protein （CHOP） and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced （P<0.05）, the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased （P<0.05）, and the protein levels of p-PI3K and p-AKT were increased in NAR group （P<0.05）. LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways.     

倒春寒危害软籽石榴树的温度及受害情况研究

以突尼斯软籽石榴为试材,在倒春寒发生前2 d开始持续监测石榴树冠层温度,倒春寒结束后调查石榴树受倒春寒危害的临界低温及寒害症状。结果表明,软籽石榴树春季生长期间,岭地、沟地和台地石榴树冠层温度降至1.4℃、-0.1℃、2.3℃,气象预报低温低于6.4℃时,石榴树芽和新梢发生低温寒害,芽尖焦枯发黑,芽体展叶受阻;新梢幼叶扭曲畸形,叶缘焦枯。     

长春市主要绿化树种适应低温冻害能力的调查与分析

以长春市部分绿地的主要园林绿化树种为对象,对其适应低温冻害的能力进行了调查与分析,结果表明:在2009～2010年冬春季节持续低温作用下,12种外来树种中未遭受冻害的有青杄等7种,占58.3%;油松、丹东桧柏、大径级垂榆、京桃、刺槐普遍遭受冻害,都有死亡植株发生,其中大径级垂榆冻死率高达63.6%。低温累积效应是导致树木遭受冻害的主要因素,种间的遗传差异亦是因素之一。乡土野生树种无冻害相发生。