蒙古扁桃种子萌发和幼苗生长对渗透胁迫的响应

以天然生长于祁连山自然保护区隆畅河自然保护站的濒危植物蒙古扁桃(Prunus mongolica)去内果皮的种子为材料,以不同渗透势PEG-6000溶液模拟干旱条件,研究了种子萌发和幼苗生长对干旱胁迫的响应特征。结果表明,随着干旱胁迫程度的加剧,蒙古扁桃种子吸胀速率、萌发率、萌发指数、活力指数、苗高和根长、组织饱和含水量等指标均表现出明显降低的趋势,而幼苗初生芽干重、根干重和根冠比均呈先升后降的趋势。蒙古扁桃种子对水势变化既敏感又抗旱,种子能够萌发的最低渗透势阈值为-0.65 MPa。干旱胁迫未能萌发的种子复水后萌发率较高。分析认为,蒙古扁桃种子萌发和幼苗生长对干旱胁迫的响应特征对种的延续具有重要意义,但在人工栽培时保证 土壤墒情应是保障建植成功的关键措施。     

植物对硒的吸收转运和形态转化机制

阐述了植物对不同形态硒的吸收、转运和形态转化机制。植物主要吸收水溶性硒,包括部分有机硒、硒酸盐和亚硒酸盐。多数研究表明植物对硒酸盐的主动吸收是通过高亲和力的硫酸盐转运子完成,最近的研究表明磷酸盐可以调节亚硒酸盐的吸收,磷酸盐转运子在亚硒酸盐的主动吸收过程中有重要作用;植物吸收的硒酸盐很快从根部转移到地上部,在叶片中被还原成亚硒酸盐,进而转化为有机硒化物进入其他组织;而亚硒酸盐及其代谢产物主要积累在根部,极少转移到地上部。进入植物体中的硒转化为含硒氨基酸和硒蛋白参与植物的代谢。     

规模化猪场处理废水与化肥配施对小麦氮素吸收利用的影响

规模化猪场处理废水富含植物营养物质,应用于作物生产可实现减少化肥用量和保护环境的双赢。本研究通过田间试验,研究了越冬期不同用量养猪处理废水(30、60、90 m3·hm-2和120 m3·hm-2)和穗期施氮水平(0、30、60 m3·hm-2和90 m3·hm-2)对小麦氮素吸收利用的影响。结果表明,猪场处理废水对小麦具有较好的氮素养分供应作用,拔节期、抽穗期、成熟期叶片SPAD值、植株含氮率都随着污水施用量增加而提高。废水施用60 m3·hm-2以上穗期配施氮90 kg·hm-2的处理花后氮素转移效率、氮素收获指数、氮素籽粒生产效率明显下降。越冬期施用养猪场处理废水60～120 m3·hm-2替代穗期施氮30～60 kg·hm-2,可以满足不同时期小麦氮素营养的需求,并且有较高的氮素积累量和利用效率。     

不同氮水平对生姜产量和品质及氮素吸收的影响

采用田间试验研究不同基础肥力土壤、不同氮肥水平对生姜产量和品质及氮素吸收的影响。结果表明,氮肥对生姜根茎产量及品质有明显的影响,合理施氮能显著提高根茎产量,改善其营养及安全品质。两种不同基础肥力土壤下,生姜根茎产量均表现为随施氮量的加大先增加后降低,呈抛物线关系,所有处理中N450获得了最高根茎产量。品质分析结果显示,施用适量氮肥能明显提高生姜根茎蛋白质、维生素C、糖分和姜精油含量,有效控制硝酸盐及亚硝酸盐含量。其中,维生素C含量,基础肥力较高土壤以N225处理最高;肥力较低土壤N450处理达到高峰。粗蛋白含量,肥力较高土壤N225处理最高,N450最低;肥力较低土壤,随施氮量的增加,根茎粗蛋白含量相应提高,N600处理达最大值。糖分和姜精油含量,两种肥力土壤下分别是N600和N450处理最高。根茎硝酸盐和亚硝酸盐含量随施氮量的增加而升高,与氮肥用量呈极显著正相关。施用氮肥后生姜地上部茎、叶和地下根茎含氮量明显提高,氮素吸收量显著增多。     

超级早、晚稻的养分吸收和根系分布特性研究

为了阐明双季超级稻的高产机理和指导合理施肥,采用品种比较的方法,研究了超级早、晚稻品种的养分吸收和根系分布特性。结果表明,超级早、晚稻品种的养分吸收量平均分别为N 170.04、224.90 kg/hm2,P 21.97、39.88 kg/hm2,K 107.48、144.47 kg/hm2,均高于对照。生育中期（穗分化至抽穗）的养分吸收量与产量密切相关。生产100 kg稻谷所需要的养分较对照低10%左右。超级早、晚稻品种根系发达,根量较大。抽穗后15 d（早稻）或20 d（晚稻）的根量与产量密切相关,其中5—10 cm的根量与养分吸收总量密切相关。说明生育中期较多的养分吸收和发达的根系是超级早、晚稻高产的重要原因。     

温度、盐度和光照强度对鼠尾藻氮、磷吸收的影响

在实验室条件下,研究了不同的温度和盐度组合,温度和光照强度组合对鼠尾藻(Sargassum thunbergii)氮、磷吸收速率的影响.结果表明,上述3个环境因子对鼠尾藻氮、磷吸收速率均有显著影响.其中,温度和盐度对鼠尾藻氮、磷吸收速率有极显著影响(P<0.01),二者交互作用也极显著(P<0.01).在盐度20、温度25℃条件下和盐度30、温度30℃条件下,鼠尾藻对氮有较高吸收速率,分别为11.26μmol/[g(dw)·h]和11.01 μmol/[g(dw)·h];在盐度40、温度15～30℃范围内对磷的吸收速率较大,达到1.5μmol/[g(dw)·h]以上.温度和光照对鼠尾藻氮、磷吸收速率均有极显著影响(P<0.01),二者交互作用极显著(P<0.01).在温度15℃和光照强度140～180μE/(m2·s)以及温度20～25℃和光照强度60～100 μE/(m2·s)条件下,鼠尾藻对氮有较高吸收速率,均在9.60 μmol/[g(dw)·h]以上;在温度25℃和光照强度60μE(m2·s)条件下,鼠尾藻对磷的吸收速率达到最大,为1.30 μmol/[g(dw)·h].本研究结果表明,鼠尾藻总体上对水体中的氮、磷均具有较高的吸收速率,且能较好地同时吸收NH4+-N和NO3--N,显示了它对海水环境中营养盐具有较强的吸收能力.     

Effect of aluminum uptake on growth and enzyme activity in Pisum sativum var. ‘Alaska’ and ‘Little Marvel’

It is usually assumed that plant tissue responses to nutritional elements are due to specific genetic differences that may exist either between inbred or closely related species. Little Marvel (dwarf) and Alaska (normal) varieties of 14‐day old pea seedlings were treated with four different concentrations of Al‐containing nutrient solution (0.0mM, 0.2mM, 0.6mM and distilled H₂O), prior to being exposed for 14 days to either DARK, LIGHT, or UV. Selected tissues (root tip, main root, main stem and proximal stem) were bioassayed for peroxidase and polyphenol oxidase enzyme activities, fresh wt vs. dry wt, water uptake and stem growth. The present study suggests that Little Marvel and Alaska pea tissue responds to high toxicity levels of Al by demonstrating an enhancement of enzymic activity. Tissue weight, growth and water uptake also show differential tissue specificity in both Little Marvel and Alaska tissue, in terms of Al toxicity response, given a particular external exposure.     

Root exudation of diclofop-methyl and triasulfuron from foliar-treated durum wheat and ryegrass

In this study, we evaluated the release of diclofop-methyl and triasulfuron from the roots of foliar-treated ryegrass and wheat. The study with ¹⁴C-diclofop-methyl indicated a basipetal translocation of foliar-applied herbicide in wheat and ryegrass. No root exudation from ¹⁴C-diclofop-methyl-treated wheat plants was observed, while 20 days after treatment (DAT) 0.2–0.9% of radioactivity absorbed by ryegrass was found exuded in the growing medium. Root exudation was stimulated three to six times by the presence of untreated wheat or ryegrass sharing the growing medium with diclofop-methyl-treated ryegrass. No subsequent uptake of exuded radiolabel by untreated plants (ryegrass or wheat) in the same pot with ¹⁴C-diclofop-methyl-treated ryegrass was observed. The study with ¹⁴C-triasulfuron indicated a basipetal translocation of foliar-applied herbicide in wheat and ryegrass and also into the growing medium. By 20 DAT, 0.5–4.2% of radioactivity absorbed by wheat or ryegrass was found exuded in the growing medium. The presence of untreated plants (wheat or ryegrass) in the same pot as triasulfuron-treated ryegrass or wheat induced exudation seven to 32 times more. The study also revealed a subsequent uptake of exuded compounds by untreated wheat or ryegrass sharing the medium of ¹⁴C-triasulfuron-treated plants. This study has demonstrated for the first time that the root exudation of exogenous compounds can be related to plant arrangement in pots. The implication is that herbicide root exudation and transfer, a form of allelopathy, could be significant in the field. A precise estimation of environmental fate, unexpected ecological side effects and residual activity of herbicides may require quantification of such exudation.     

Patterns and drivers of seasonal water sources for artificial sand-fixing plants in the northeastern Mu Us sandy land, Northwest China

Understanding plant water-use patterns is important for improving water-use efficiency and for sustainable vegetation restoration in arid and semi-arid regions. However, seasonal variations in water sources and their control by different sand-fixing plants in water-limited desert ecosystems remain poorly understood. In this study, stable isotopic ratios of hydrogen (δ²H) and oxygen (δ¹⁸O) in precipitation, soil water, groundwater, and xylem water were determined to document seasonal changes in water uptake by three representative plant species (Pinus sylvestris var. mongolica Litv., Amygdalus pedunculata Pall., and Salix psammophila) in the northeastern Mu Us sandy land, Northwest China. Based on the depth distribution and temporal variation of measured gravimetric soil water content (SWC), the soil water profile of the three species stands was divided into active (0.01 g g^-1 < SWC < 0.08 g g^-1, 20% < coefficient of variation (CV) < 45%), stable (0.02 g g^-1 < SWC < 0.05 g g^-1, CV < 20%), and moist (0.08 g g^-1 < SWC < 0.20 g g^-1, CV > 45%) layers. Annually, P. sylvestris, A. pedunculata, and S. psammophila obtained most water from deep (59.2% ±9.7%, moist layer and groundwater), intermediate (57.4% ±9.8%, stable and moist layers), and shallow (54.4% ±10.5%, active and stable layers) sources, respectively. Seasonally, the three plant species absorbed more than 60% of their total water uptake from the moist layer and groundwater in the early (June) dry season; then, they switched to the active and stable layers in the rainy season (July–September) for water resources (50.1%–62.5%). In the late (October–November) dry season, P. sylvestris (54.5%–66.2%) and A. pedunculata (52.9%–63.6%) mainly used water from stable and moist layers, whereas S. psammophila (52.6%–70.7%) still extracted water predominantly from active and stable layers. Variations in the soil water profile induced by seasonal fluctuations in precipitation and groundwater levels and discrepancies in plant phenology, root distribution, and water demand are the main factors affecting the seasonal water-use patterns of artificial sand-fixing plants. Our study addresses the issue of plant water uptake with knowledge of proportional source-water use and reveals important implications for future vegetation restoration and water management in the Mu Us sandy land and similar desert regions around the world.     

Effect of levels and sources of nitrogen on concentration and uptake of nitrogen by a high yielding variety and a hybrid of rice

Field experiments were made on a sandy clay loam soil at the Indian Agricultural Research Institute, New Delhi to study the effect of levels and sources of nitrogen on concentration and uptake of nitrogen by a high yielding variety Pusa 834 and a hybrid PRH3 of rice. Nitrogen concentration in hybrid PRH 3 remained lower than in Pusa 834, but N uptake was significantly more in the hybrid PRH 3. Nitrogen fertilization increased N concentration as well as N uptake by rice. At 30 days after transplanting (DAT) N uptake was more in Pusa 834, but at 60 DAT and at harvest hybrid PRH 3 recorded significantly more N uptake than Pusa 834. Use of neem oil blended urea (PNGU) and neem coated urea (NCU) increased N concentration and uptake by rice in both Pusa 834 and hybrid PRH 3. Use of neem coated/blended urea is recommended for rice.