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本文用液氮冷冻法比较了不同保护剂和不同冻存途径对伊氏锥虫云南株进行保存后,虫株在存活率、活力、感染力、致病力和药敏性方面的变化。我们发现在以二甲基亚砜为主要保存剂时,二甲基亚砜浓度低对保存效果不利,而浓度为5%时,保存712天后,虫体成活率仍达96%;当以甘油为保存剂时,若其浓度低虫体成活率低,当浓度为20%时,保存365天后虫体存活率有91.4%。在不同冻存途径试验中发现,先在液氮气相中距液氮面4cm以上处放置1h,再降到距液氮面4cm处放置1h,然后进入液氮效果最好。用此种保存方法和冻存途径后,虫体活力强,其感染力、致病性和药敏性都没有发生明显变化。 相似文献
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以碘活化的六乙基亚磷酰三胺为磷酰化及环化试剂 ,经“一锅法”合成了邻苯二甲酰亚胺乙氧基环甘油硫代磷脂缀合物 ,收率达 90 %左右 相似文献
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在制作小尾寒羊冻精颗粒时,对其冷冻稀释液和甘油浓度进行了研究。结果表明,2#稀释液效果最好(P<0.05),甘油浓度为6%时的冷冻效果最佳(P<0.01);在40-50℃解冻后,其活力可达0.5以上。 相似文献
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Neal T. Wasserman Gavin Kennard Zachary N. Cochrane Lisa M. Felchle 《Veterinary ophthalmology》2013,16(1):20-24
Objective To evaluate and compare the effects of oral isosorbide and glycerol on intraocular pressure (IOP), serum osmolality (SOSM), and blood glucose (BG) in normal dogs. Methods Ten normal dogs were administered an oral dose of either isosorbide (1.5 g/kg), glycerol (1.5 g/kg) or control (water, 2 mL/kg) in a double blind protocol. Prior to dosing, baseline IOP, SOSM, and BG were measured in all dogs. IOP was subsequently evaluated every 30 min for 6 h post‐dosing. BG and SOSM were reassessed at times 1, 2, 4, and 6 h post‐dosing. After 1‐week washout periods, every dog was subject to each of the three treatments. The dogs were held NPO for 4 h after dosing. Results The maximal decrease in IOP was 17% by 1 h and 13.5% by 30 min after glycerol and isosorbide administration, respectively. However, the overall changes in IOP were not significant when compared to the controls. SOSM increased above baseline after dosing with glycerol but decreased after isosorbide, which difference was significant at 1, 2, and 4 h post‐administration. BG significantly increased after administration of glycerol relative to the control but was not significantly affected by isosorbide. Conclusions Neither glycerol nor isosorbide significantly affected IOP when compared to the control. However, glycerol induced significant elevations in both BG relative to the control and SOSM relative to isosorbide. 相似文献
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Niels Oksbjerg Poul Henckel Søren Andersen Birthe Pedersen Bjarne Nielsen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(4):187-192
Heritability values of glycerol, glycogen and pigment concentrations measured on muscle biopsy samples from longissimus dorsi obtained from 85?kg boars of Danish purebred pigs (Landrace, Yorkshire, and Duroc) and their genetic correlations to performance and meat quality traits were calculated. The heritability values of glycogen, pigment, and glycerol were 0.38±0.02, 0.17±0.02, and 0.065±0.016, respectively. Glycogen was negatively related to the feed conversion ratio (FCR) (r g =?0.25±0.06) and ultimate meat pH (r g =?0.41±0.04), and positively to the carcass meat percentage (r g =0.35±0.04), L* (lightness of meat, r g =0.32±0.04) and a* (redness of meat, r g =0.12±0.03). Pigment was positively related to the FCR (r g =0.12±0.05), the meat percentage (r g =0.28±0.05), and a* (r g =0.59±0.04), and negatively to the L* (r g =?0.46±0.06). The concentrations of pigment and glycogen (r g =0.27±0.05) were interrelated. Based on the heritability values and the signs of the genetic correlations the present data suggest that it is possible to select for higher ultimate pH and improved colour by selection for either lower muscle glycogen or higher pigment concentration, respectively. However, the positive genetic correlation between these two traits may restrict the efficiency of simultaneous selection for lower glycogen and higher pigment. All genetic parameter estimates presented are calculated across three purebreds. Values for each breed are warranted for use in pig production. However, this requires more animals per breed. 相似文献
7.
K. K. Baruah A. Dhali A. Mech B. Bora J. Das R. Bora M. Mondal B. C. Sarmah B. C. Deka C. Rajkhowa 《Journal of animal physiology and animal nutrition》2013,97(6):1051-1058
The effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa was investigated. Semen samples were collected from five healthy mithun bulls through rectal massage method and cryopreserved in liquid nitrogen. The samples were diluted in Tris–egg yolk–glycerol extender, equilibrated for 4 h at 4 °C and loaded into 0.50‐ml straws. The straws were then frozen in liquid nitrogen vapour for 10 min and finally plunged into liquid nitrogen for storage. The required amount of glycerol was added into the diluted samples either in a single dose (3%, 4%, 5%, 6% or 7%; added at 37 °C immediately before equilibration) or in split doses (5%, 6% or 7%; the total amount was divided into four equal parts, and a part was added at 37 °C immediately before equilibration, and the remaining parts were added subsequently at 1, 2 and 3 h of equilibration at 4 °C). In the single‐dose addition method, following freeze‐thawing, greater (p < 0.05) motility (%) and proportion of live spermatozoa with intact acrosome (LSIA, %) in 5% glycerol (40.6 ± 1.7 and 43.4 ± 1.8 respectively) and lesser (p < 0.05) total morphological abnormalities (%) in 5% (14.1 ± 0.8) and 6% (13.7 ± 1.0) glycerol were observed compared to the other glycerol concentrations. In the split‐dose addition method, following freeze‐thawing, greater (p < 0.05) motility (%) and LSIA proportion (%) were found in 5% (50.2 ± 1.9 and 53.3 ± 1.8 respectively) compared to 6% or 7% glycerol, but the total morphological abnormalities were not different among the glycerol concentrations. In addition, in all the glycerol concentrations, better (p < 0.05) post‐freeze‐thaw motility and LSIA proportions were observed when glycerol was added in split doses compared to a single dose. In conclusion, Tris–egg yolk extender with 5% glycerol added in split doses was found most suitable for cryopreserving mithun sperm. 相似文献
8.
用PCR的方法,从产甘油假丝酵母WL2002—5中扩增出了产甘油关键酶3-磷酸甘油脱氢酶(glycerol 3-phosphate dehydrogenase)和3-磷酸甘油磷酸酶(glycerol 3-phosphate phosphatase)的基因GPD和GPP。对这两个基因测序,发现基与酿酒酵母(5accharomyces.cereuisiae)的GPD、GPP基因的相似性达99%。并构建了其在植物中的表达载体。 相似文献
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W.R. Driedzic J.L. West D.H. Sephton J.A. Raymond 《Fish physiology and biochemistry》1998,18(2):125-134
Rainbow smelt (Osmerus mordax) tolerate temperatures close to the freezing point of sea water, in part, through the use of glycerol as an antifreeze. Potential mechanisms for glycerol production by liver were assessed by comparing activities of key enzym es of carbohydrate and amino acid metabolism in rainbow smelt to those in Atlantic tomcod (Microgadus tomcod) and smooth flounder (Liopsetta putmani). The latter two species inhabit the same environment but do not maintain high levels of blood glycerol. The enzyme profile of liver from rainbow smelt is substantially different from those of the other species and is poised for glycerol production. With respect to carbohydrate metabolism, glycerol-3-phosphate dehydrogenase activity in rainbow smelt liver was 156 µmoles min-1 g-1, a level which was 28 and 12-fold higher than activities in tomcod and flounder liver, respectively. Glycerol-3-phosphatase activity in smelt liver was 1.95 µmol min-1 g-1. This activity was 2.7 and 5.4-fold higher than those in tomcod and flounder liver, respectively. As such, the production of glycerol appears to be dependent upon the concerted action of glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase. The enzyme profile also suggests that amino acids are a potential source of carbon for glycerol. Aspartate aminotransferase activity in rainbow smelt was 7 to 14-fold higher in comparison to tomcod and flounder liver, respectively. Activities of alanine aminotransferase and glutamate dehydrogenase in liver were between 2 and 3-fold higher in rainbow smelt than in the other two species. Finally, it is shown that in vitro preparations of smelt liver sections produce glycerol at 0°C lending support to the concept that liver is a site of glycerol synthesis in vivo. 相似文献