用鱼肌核酸指示水体污染的初步研究

选择了4种家鱼作指示生物，用紫外分光光度法测定其白肌中RNA和DNA的含量。结果表明：同种鱼，污染区的RNA含量显著低于对照区，白鲢和鳊鱼白肌中RNA／DNA低于对照区；不同种的鱼，污染区鱼肌核酸比对照区下降的程度有鳊鱼＞白鲢＞鲫鱼和鲤鱼的趋势。鱼肌核酸下降同水体重金属污染有一定联系，RNA是比RNA／DNA更合适的指标，鳊鱼和白鲢是比鲫鱼和鲤鱼更敏感的指示生物。     

DBD技术处理鱼粉厂恶臭气体的研究

为了去除鱼粉加工中产生的大量恶臭气体,利用DBD低温等离子体技术对硫化氢和三甲胺进行去除,并在单因素试验的基础上,采用Box-Behnken设计方法,考察了电压、放电频率、处理时间、初始浓度及其交互作用对硫化氢和三甲胺去除率的影响。结果表明,响应面法优化所得的最佳工艺条件为:电压10.6k V,放电频率8.91KHz,处理时间0.26s,硫化氢初始浓度0.76mg·m-3,三甲胺初始浓度为19.8mg·m-3。此条件下硫化氢和三甲胺的去除率均可达100%,能耗为1.77×10-2KW·h·m-3;同时,验证试验结果与模型预测值吻合。本研究为DBD技术在鱼粉厂去除恶臭气体中的应用奠定了基础。     

Numerical simulation of deformations and forces of a floating fish cage collar in waves

The dynamic behavior of a fish cage collar in waves was investigated using a numerical model based on the finite element method. The floating collar and mooring system were divided into a series of line segments modeled by straight massless model segments with a node at each end. To verify the validity of the numerical model, research data from other authors were cited and compared with the simulated results, the comparison of results showed a good agreement. The numerical model was then applied to a dynamic simulation of a floating cage collar in waves to analyze its elastic deformation and mooring line tension. The simulated results indicated that the greatest deformation of the collar taken place in the position of the mooring line connection point when incident waves were in the same direction. An increase in the length of mooring line would help to decrease the mooring line tension of the collar. Furthermore, the effects of collar dimension, including collar circumference, pipe diameter in cross-section, and pipe thickness, on the dynamic behavior of the floating collar were discussed. The results of this study provided a better understanding of the dynamic behavior of the fish cage collar.     

Extensive dispersal of Roanoke logperch (Percina rex) inferred from genetic marker data

The dispersal ecology of most stream fishes is poorly characterised, complicating conservation efforts for these species. We used microsatellite DNA marker data to characterise dispersal patterns and effective population size (N_e) for a population of Roanoke logperch Percina rex, an endangered darter (Percidae). Juveniles and candidate parents were sampled for 2 years at sites throughout the Roanoke River watershed. Dispersal was inferred via genetic assignment tests (ATs), pedigree reconstruction (PR) and estimation of lifetime dispersal distance under a genetic isolation‐by‐distance model. Estimates of N_e varied from 105 to 1218 individuals, depending on the estimation method. Based on PR, polygamy was frequent in parents of both sexes, with individuals spawning with an average of 2.4 mates. The sample contained 61 half‐sibling pairs, but only one parent–offspring pair and no full‐sib pairs, which limited our ability to discriminate natal dispersal of juveniles from breeding dispersal of their parents between spawning events. Nonetheless, all methods indicated extensive dispersal. The AT indicated unrestricted dispersal among sites ≤15 km apart, while siblings inferred by the PR were captured an average of 14 km and up to 55 km apart. Model‐based estimates of median lifetime dispersal distance (6–24 km, depending on assumptions) bracketed AT and PR estimates, indicating that widely dispersed individuals do, on average, contribute to gene flow. Extensive dispersal of P. rex suggests that darters and other small benthic stream fishes may be unexpectedly mobile. Monitoring and management activities for such populations should encompass entire watersheds to fully capture population dynamics.     

Multiple watershed alterations influence fish community structure in Great Plains prairie streams

Stream fish distributions are commonly linked to environmental disturbances affecting terrestrial landscapes. In Great Plains prairie streams, the independent and interactive effects of watershed impoundments and land cover changes remain poorly understood despite their prevalence and assumed contribution to declining stream fish diversity. We used structural equation models and fish community samples from third‐order streams in the Kansas River and Arkansas River basins of Kansas, USA to test the simultaneous effects of geographic location, terrestrial landscape alteration, watershed impoundments and local habitat on species richness for stream‐associated and impoundment‐associated habitat guilds. Watershed impoundment density increased from west to east in both basins, while per cent altered terrestrial landscape (urbanisation + row‐crop agriculture) averaged ~50% in the west, declined throughout the Flint Hills ecoregion and increased (Kansas River basin ~80%) or decreased (Arkansas River basin ~30%) to the east. Geographic location had the strongest effect on richness for both guilds across basins, supporting known zoogeography patterns. In addition to location, impoundment species richness was positively correlated with local habitat in both basins; whereas stream‐species richness was negatively correlated with landscape alterations (Kansas River basin) or landscape alterations and watershed impoundments (Arkansas River basin). These findings suggest that convergences in the relative proportions of impoundment and stream species (i.e., community structure) in the eastern extent of both basins are related to positive effects of increased habitat opportunities for impoundment species and negative effects caused by landscape alterations (Kansas River basin) or landscape alterations plus watershed impoundments (Arkansas River basin) for stream species.     

Ecological impacts of small hydropower plants on headwater stream fish: from individual to community effects

Hydroelectricity is increasingly used worldwide as a source of renewable energy, and many mountain ranges have dozens or hundreds of hydropower plants, with many more being under construction or planned. Although the ecological impacts of large dams are relatively well known, the effects of small hydropower plants and their weirs have been much less investigated. We studied the effects of water diversion of small hydropower plants on fish assemblages in the upper Ter river basin (Catalonia, NE Spain), which has headwater reaches with good water quality and no large dams but many of such plants. We studied fish populations and habitat features on control and impacted reaches for water diversion of 16 hydropower plants. In the impacted reaches, there was a significantly lower presence of refuges for fish, poorer habitat quality, more pools and less riffles and macrophytes, and shallower water levels. We also observed higher fish abundance, larger mean fish size and better fish condition in the control than in impacted reaches, although the results were species‐specific. Accordingly, species composition was also affected, with lower relative abundance of brown trout (Salmo trutta) and Pyrenean minnow (Phoxinus bigerri) in the impacted reaches and higher presence of stone loach (Barbatula quignardi) and Mediterranean barbel (Barbus meridionalis). Our study highlights the effects of water diversion of small hydropower plants from the individual to the population and community levels but probably underestimates them, urging for further assessment and mitigation of these ecological impacts.     

Salinity and geomorphology drive long‐term changes to local and regional fish assemblage attributes in the lower Pecos River,Texas

River systems throughout arid regions worldwide have been heavily impacted by human activities, resulting in long‐term ecological consequences. The lower Pecos River in the Trans‐Pecos region of Texas is no exception, having undergone anthropogenic changes that include decreased flow, elevated salinity, species loss and species invasion. We compared historical and contemporary fish assemblage attributes from the Pecos River at local (site‐specific) and regional (Trans‐Pecos region) scales across a 24‐year time period. Fish assemblage data were collected in October 1987 and 2011, by seining at 15 sites spanning 430 km of the river in Texas. Additionally, we examined contemporary environmental conditions to determine species–environment relationships. We found that fish assemblages were significantly different between time periods, likely due to increased salinisation in the upper half of the study region. Decreased species richness, species replacement and increases in euryhaline species were documented in the upstream sites. Freshwater springs lower the salinity and maintain flows in the downstream reach, allowing for maintenance of the native fish fauna. Careful management of regional aquifers, irrigation practices and petroleum waste water will be necessary for protecting biodiversity and environmental flows in the lower Pecos River.     

不同植物油对大规格吉富罗非鱼生长性能、组织脂肪酸组成的影响

本试验旨在探究大规格吉富罗非鱼(GIFT)对不同植物油的利用效果,筛选出大规格吉富罗非鱼饲料中合适的植物脂肪源。分别以大豆油(SO)、棕榈油(PO)、棉籽油(CO)、菜籽油(RO)、磷脂油(SL)和大豆油∶棕榈油∶棉籽油∶菜籽油∶磷脂油=1∶1∶1∶1∶1的混合油(MIX)为脂肪源配制6种等氮等脂的试验饲料,饲喂初始体重为(229.92±0.31)g的吉富罗非鱼8周。每种试验饲料投喂4个网箱(重复),每个网箱放养50尾。结果表明:对于增重率和特定生长率,棉籽油组和混合油组显著低于菜籽油组(P0.05)。菜籽油组饲料系数显著低于混合油组(P0.05),其他各组间无显著差异(P0.05)。混合油组蛋白质效率显著低于菜籽油组和磷脂油组(P0.05)。混合油组肝体指数显著高于棕榈油组和菜籽油组(P0.05)。大豆油组、菜籽油组、棕榈油组和磷脂油组在增重率、特定生长率和饲料系数方面没有显著差异(P0.05)。大豆油组和菜籽油组肌肉饱和脂肪酸总量显著低于其他各组(P0.05)。对于肌肉中n-3系脂肪酸/n-6系脂肪酸,菜籽油组显著高于其他各组(P0.05),棉籽油显著低于其他各组(P0.05)。棉籽油组肝脏饱和脂肪酸总量显著高于其他各组(P0.05),菜籽油组则显著低于其他各组(P0.05);对于肝脏n-3/n-6,菜籽油组显著高于其他各组(P0.05)。各组肌肉脂肪酸组成与饲料脂肪酸组成的相关系数均高于肝脏脂肪酸组成与饲料脂肪酸组成的相关系数。在饲料脂肪酸组成与肌肉或肝脏脂肪酸组成的相关系数中,均以棕榈油组最大,磷脂油组最小。由此得出,大豆油、棕榈油、菜籽油和磷脂油可以作为大规格吉富罗非鱼(体重为230~790 g)饲料中良好的脂肪源。大规格吉富罗非鱼肌肉中脂肪酸组成一定程度上被饲料脂肪酸组成影响,但肝脏脂肪酸组成与饲料脂肪酸组成的相关性较小。     

Kruppel样因子15对脂肪沉积和肌纤维类型的影响及其可能机制

Kruppel样因子15(KLF15)是一种新发现的Kruppel样因子家族(KLFs)成员,为一种真核锌指蛋白转录因子。KLF15在动物体内呈现多组织表达特性,该基因过表达能促进动物脂肪沉积,且能影响肉质性状候选基因和Ⅰ型肌纤维基因的表达。因此,深入研究KLF15对脂肪沉积和肌纤维类型的影响及其可能机制,将为改善动物肉品质提供新的思路。本文综述了KLF15的发现、结构特征、表达规律及其与脂肪沉积和Ⅰ型肌纤维之间的关系。     

Assemblage patterns of fish functional groups relative to habitat connectivity and conditions in floodplain lakes

Miyazono S, Aycock JN, Miranda LE, Tietjen TE. Assemblage patterns of fish functional groups relative to habitat connectivity and conditions in floodplain lakes.
Ecology of Freshwater Fish 2010: 19: 578–585. © 2010 John Wiley & Sons A/S Abstract – We evaluated the influences of habitat connectivity and local environmental factors on the distribution and abundance patterns of fish functional groups in 17 floodplain lakes in the Yazoo River Basin, USA. The results of univariate and multivariate analyses showed that species–environmental relationships varied with the functional groups. Species richness and assemblage structure of periodic strategists showed strong and positive correlations with habitat connectivity. Densities of most equilibrium and opportunistic strategists decreased with habitat connectivity. Densities of certain equilibrium and opportunistic strategists increased with turbidity. Forested wetlands around the lakes were positively related to the densities of periodic and equilibrium strategists. These results suggest that decreases in habitat connectivity, forested wetland buffers and water quality resulting from environmental manipulations may cause local extinction of certain fish taxa and accelerate the dominance of tolerant fishes in floodplain lakes.