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为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。 相似文献
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牛羊4种寄生虫病联合诊断技术的研究与应用 总被引:1,自引:1,他引:0
疫区牛羊同时寄生有捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫等多种寄生虫。目前血清学诊断方法对这 4种寄生虫病的检测需 2~ 4次操作。将捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫抗原分别以 0 1 5 ,0 0 4 ,0 0 6和 0 1 3μg/ μL的最佳浓度依次定点在同一硝酸纤维素膜条上 ,制成 4种寄生虫病联合诊断膜。用黄牛IgG ,水牛IgG和山羊IgG联合免疫兔 ,获得了高效价兔抗黄牛、水牛和山羊 (简称兔抗牛羊 )IgG抗血清。应用兔抗牛羊IgG抗血清连接酶标SPA ,建立了 1份血纸能同时用于检测牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病抗体技术。经浙江、湖北、四川、安徽等省应用 ,该项技术具有省工、省时、经济、实用等优点。适宜于牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病流行区的普查或监测 相似文献
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Spinosad, a reduced-risk commercial insecticide derived from a bacterial fermentation product, possesses both contact and oral toxicities against insects. Contact toxicity of spinosad to adults of Rhyzopertha dominica (F), Sitophilus oryzae (L), and Tribolium castaneum (Herbst) was evaluated by exposure for 24 or 48 h to treated glass Petri dishes. Adults were exposed to different deposits (0.001-0.79 mg cm(-2)) of spinosad in 24-h tests and to deposits of 0, 0.0016 and 0.016mg cm(-2) in 48-h tests. Rhyzopertha dominica was most susceptible to spinosad in 24- and 48-h tests, followed by S. oryzae, and T. castaneum. The 24-h LD50 values were 0.0004, 0.077 and 0.189mg cm(-2) for R. dominica, S. oryzae, and T. castaneum, respectively. All R. dominica adults were dead following 48 h exposure to both spinosad deposits, whereas mortality of S. oryzae and T. castaneum ranged from 10 to 85% and 12 to 48%, respectively. Rhyzopertha dominica, T. castaneum, and O. surinamensis adults were exposed for 14 days to whole wheat, cracked wheat and wheat flour treated with 0, 0.1 and 1.0 mg kg(-1) of spinosad. Rhyzopertha dominica adults were highly susceptible to spinosad, followed by O. surinamensis and T. castaneum. Immatures (eggs and larvae) of T. castaneum and O. surinamensis exposed for 14 days were more susceptible on spinosad-treated whole wheat than on treated cracked wheat and wheat flour. This is the first report documenting contact activity of spinosad, and the effect of grain condition on spinosad toxicity, to stored-product insects. 相似文献
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Martínez Vidal JL Egea González FJ Garrido Frenich A Martínez Galera M Aguilera PA López Carrique E 《Pest management science》2002,58(8):784-790
Principal component analysis (PCA) was applied to the gas chromatographic data obtained from 23 different greenhouse trials. This was used to establish which factors, including application technique (very small, small, medium and large drop-size), crop characteristics (short/tall, thin/dense) and pattern application of the operator (walking towards or away from the treated area) are relevant to the dermal exposure levels of greenhouse applicators. The results showed that the highest exposure by pesticides during field applications in greenhouses, in the climatic conditions and in the crop conditions typical of a southern European country, occurs on the lower legs and front thighs of the applicators. Similar results were obtained by hierarchical cluster analysis (HCA). Drop-size seems to be very important in determining total exposure, while height and density of crops have little influence on total exposure under the conditions of the present study. No pesticide type is a major factor in total exposure. The application of multiple regression analysis (MRA) allowed assessment of the relationships between the pesticide exposure of the less affected parts of the body with the most affected parts. 相似文献
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The purpose of this study was to evaluate oxidative stress and neurotoxic potential of organophosphorus (OP) insecticide diazinon in the sentinel freshwater fish, Oreochromis niloticus. Antioxidant and acetylcholinesterase (AChE) enzyme activities and malondialdehyde (MDA) and protein levels were measured spectrophotometrically in gill, kidney, alimentary tract, and muscle tissues of fish treated with sub-lethal diazinon concentrations for 1, 7, 15, and 30 days. Dose-dependent inhibitions of AChE were observed in all the experimental fish. On the contrary of alimentary tract, MDA levels were elevated in kidney and muscle and gill was not affected. AChE and MDA levels intercorrelated in kidney and muscle tissues. Diazinon had increased superoxide dismutase (SOD) activities in all the tissues, while kidney was the most affected tissue. Tissue-specific alterations were observed on catalase (CAT) and glutathione peroxidase (GPx) activities; however, the activities were not changed in gill and muscle tissues for GPx and in gill, muscle, and kidney tissues for CAT. Protein levels decreased in kidney, muscle, and alimentary tract, while increased in gill and alimentary tract in 15 days. With respect to these results, diazinon has oxidative and neurotoxic potentials in O. niloticus. Observed changes with diazinon treatment were generally tissue-specific and dose-dependent. 相似文献
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在实验室条件下,测定了甲霜灵和霜霉威复配剂对棉疫病菌(Phytophthora boehmeriae)的联合毒力。结果表明,复配剂MPA、MPB、MPC对棉疫病菌的DC5。分别为0.0555,0.1044μg/mL和0.0845μg/mL,对照单剂甲霜灵和霜霉威对棉疫病菌的EC50分别为0.0391μg/mL和10.6183μg/mL;联合毒力分析表明,甲霜灵和霜霉威按1:1.5复配有增效作用,按1:1和1.5:1复配有相减作用。 相似文献
29.
In high-income countries depression and cardiovascular diseases were predicted to be the two leading causes of DALYs in the year 2030. Private-life stress fosters both kinds of diseases. Scientific findings already show that forest exposure has stress-reducing effects. Particularly in Japan, people have practiced forest bathing to improve their health. The German population also has a strong connection to its forests, and forest law allows forest access, regardless of ownership structure. Hence, the question arises of whether forest exposure could be used in Germany as a kind of stress-coping strategy. To a certain degree, the success of such a strategy in Germany would require the participation of the stakeholders that are active in both the health and the forestry sectors. Therefore, it seems necessary to gain insight into German forest and health professionals' understanding and attitude concerning forest-related health benefits. For this reason, in this pilot study, guideline interviews with professionals of both sectors and with professionals standing in between these professions were conducted, recorded and transcribed. On the one hand, each professional’s presumptions regarding the health-fostering effects of forests were investigated, derived from their subjective certainty that forest exposure has health-fostering effects. In addition, a thought experiment was used to estimate the level of willingness to cooperate with each other in order to motivate people to be physically active in forests. For analysis, Mayring’s qualitative content analysis and a frequency analysis (MaxQDA) were applied. Findings show that most of the interviewed professionals presume forests to have health-fostering effects. Furthermore, something derived from the statements within the context of the thought experiment was that most professionals seem to be willing to cooperate with the other sector. Hence, it might be conceivable that forest exposure may be part of a German stress-coping strategy. 相似文献
30.
稻油轮作区驱动圆盘犁对置组合式耕整机设计与试验 总被引:3,自引:0,他引:3
针对长江中下游稻油轮作区油菜种植时土壤黏重板结、秸秆量大、播种作业需同步开畦沟的农艺要求,考虑传统耕整作业耕层浅、功耗大的不足,依据驱动圆盘犁组与传统铧式犁相比,不易缠草堵塞、通过性好、牵引阻力小的特征,设计了用于油菜播种的驱动圆盘犁对置组合式耕整机。提出了主动式对置犁耕与被动式开畦沟、碎土、平整相结合的联合耕整作业方案,分析了对称布置的圆盘犁组的动力学和运动学特性,确定了其主要结构和工作参数。根据犁体曲面成形原理,设计了开畦沟前犁犁体曲面;依据组合式船型开沟器与土壤挤压互作机制的分析,确定了开畦沟区域宽度为350 mm时,开畦沟系统作业后可有效保证畦沟和种床厢面质量。耕深稳定性试验表明,整机作业实际耕深与限深深度基本一致,耕深稳定性系数均在90%以上。厢面质量试验表明,开畦沟系统在中间开畦沟区域能开出沟宽241.6~293.5 mm,沟深328.6~370.8 mm的梯形沟。经组合式船型开沟器挤压的土壤对犁沟的实际填埋率高于87.67%,碎土辊作业后厢面平整度为22.45~26.70 mm,碎土率为60.14%~68.37%。正交试验结果表明,整机较优工作参数为:限深深度为180 mm,机组前进速度为3.5 km/h,圆盘犁组转速为160 r/min,此时整机功耗为24.37 k W,相比传统旋耕方式的油菜播种种床整备机具的功耗降低了37.67%,秸秆埋覆率为92.78%,碎土率为66.74%,厢面平整度为24.18 mm,土壤对犁沟平均填埋率为92.3%,满足油菜播种的农艺要求。 相似文献