植物病原细菌hrp基因研究现状和展望

从ｈｒｐ（过敏性反应和致病性）基因簇，ＤＮＡ序列的同源性，基因表达调控与ａｖｒ基因（无毒基因）的关系和基因的功能等几个方面较为详细地综述了植物病原细菌ｈｒｐ基因的研究现状，并分析了ｈｒｐ基因研究的未来趋势。     

香蕉柠檬酸合酶基因MaGCS的克隆及生物信息学分析

采用RACE-PCR的方法首次在香蕉中获得一个柠檬酸合酶基因的cDNA序列。经序列测定和Blastx比对分析表明，该cDNA全长1 885bp，编码513个氨基酸残基，具有植物柠檬酸合酶基因的特征结构域，并与其他植物来源的乙醛酸循环体的柠檬酸合酶具有很高的序列相似性，将其命名为MaGCS(Musa glyoxysomal citrate synthase)，并对其进行生物信息学分析，初步预测其理化性质及功能等。     

地震灾害对农民收入影响的评估分析

5.12大地震给四川农民的收入带来了巨大的影响。在收集整理并分析2000~2007年四川农民收入的实际数据的基础上,根据灰色系统理论,建立了基于GM（1,1）的农民收入评估模型,并用该模型对四川农民2008年收入进行了评估研究,得到了一些有用的结论。     

葡萄A病毒外壳蛋白基因克隆及分子变异分析

为获得葡萄A病毒(Grapevine virus A,GVA)外壳蛋白(CP)基因,并为开展GVA-CP介导的抗病毒转基因研究提供基础,本研究从葡萄休眠枝条中扩增获得12个GVA分离物的CP基因序列,全长597nt,编码198个氨基酸。所得分离物间核苷酸和氨基酸序列同源性分别为83.9%~99.8%和92.4%~99.5%,与GenBank中12个GVA CP序列比较,核苷酸和氨基酸序列同源性分别为71.0%~93.1%和81.8%~98.0%。系统进化分析表明,本研究12个分离物CP序列与已报道的GVA Ⅰ组亲缘关系最近。种群变异分析表明,GVA种群具有多种变异体类型,并且多个样品存在不同变异体类型的复合侵染。但绝大多数变异体克隆聚集在GVA Ⅰ组分支,有60%的克隆序列间同源性较高,并且聚集在同一个次级分支Ⅰ Aa,表明其为该GVA种群的优势序列。克隆的GVA CP基因以及优势序列群体的分析为葡萄抗病毒转基因研究奠定了基础。     

基于转录组数据的害虫抗药性综合检测方法

为建立基于转录组数据的害虫抗药性检测方法,以3种重要害虫家蝇Musca domestica、白纹伊蚊Aedes albopictus和小菜蛾Plutella xylostella的8个抗性/敏感种群的转录组数据为对象,使用已开发的乙酰胆碱酯酶抗性突变检测程序ACE检测不同种群中的乙酰胆碱酯酶抗药性突变,并使用Bowtie 2软件和R程序包DESeq2检测其解毒酶基因的表达量变化,分析这3种害虫对有机磷类和氨基甲酸酯类杀虫剂的抗性分子基础。结果显示,在家蝇2个抗性种群KS8S3和ALHF中检测到ace2基因上发生了G227A抗药性突变,突变频率分别为0.318和1.000;在白纹伊蚊抗性种群Tem-GR中检测到CCEae3a基因的表达量较敏感种群Par-GR上调了7.175倍;在小菜蛾抗性种群ZZ中检测到ace1基因上发生A201S和G227A抗药性突变,突变频率分别为0.656和0.692。根据上述突变频率和解毒酶基因表达量变化评估的3种害虫种群抗药性情况与其之前的报道基本相符,表明基于转录组数据同时对靶标抗性机制和代谢抗性机制进行检测的害虫抗药性综合检测方法可以很好地反映害虫种群的抗药性状况。     

Protective effect of C1q/tumor necrosis factor-related protein 3 on vascular endothelium in rats with hyperuricemia

AIM To study whether C1q/tumor necrosis factor （TNF）-related protein 3 （CTRP3）protect vascular endothelium in rats with hyperuricemia and its potential mechanisms. METHODS An animal model of hyperuricemia was established by using male SD rats drinking 10% fructose water （n=10）. The rats drinking normal water served as normal controls （n=10）. After 12 weeks， the rats were given a single injection with Ad-CTRP3 or Ad-GFP. The experiment was ended at 14th day after transfection.The serum levels of uric acid and nitric oxide （NO） were evaluated. The serum contents of TNF-α and interleukin-6 （IL-6） were measured by ELISA. HE staining and TUNEL assay were used to assess the morphological changes of intima and apoptosis of endothelial cells in thoracic aorta， respectively. The mRNA levels of endothelial nitric oxide synthase （eNOS）， TNF-α and IL-6 were detected by RT-qPCR. The protein levels of CTRP3 and Toll-like receptor 4 （TLR4） were determined by Western blot. RESULTS Compared with normal control group， the rats with hyperuricemia showed lower CTRP3 and higher TLR4 protein levels in the thoracic aorta （P<0.05）. Hyperuricemic rats had higher serum contents of uric acid， TNF-α and IL-6 （P<0.05）. Also， the intima structure disturbance of thoracic aorta， increased apoptotic rate， higher mRNA levels of TNF-α and IL-6 as well as lower mRNA levels of eNOS were observed （P<0.05）. By contrast， CTRP3 over-expression decreased TLR4 protein levels， reduced inflammatory cytokines， and obviously improved the morphology and function of thoracic aorta in the rats with hyperuricemia. CONCLUSION CTRP3 protect vascular endothelium in rats with hyperuricemia maybe via down-regulation of TLR4- mediated inflammatory signaling pathway.     

Effects of oxidized high-density lipoprotein on membrane fluidity and the expression of lymphocyte function associated antigen-1 of monocyte

AIM: To investigate the effects of oxidized high-density lipoprotein (oxHDL) on membrane fluidity and the expression of lymphocyte function associated antigen(LFA-1) of monocyte. METHODS: The membrane fluidity of THP-1 cells was assayed by fluorescence anisotropy with DPH (1,6-dipheny-1,3,5-hexatriene), a fluorescent probe; The LFA-1 expression on THP-1 cells were assayed by flow cytometry with indirect immunofluorescence.RESULTS: The membrane fluidity of THP-1 cells was reduced by 45% and 52% respectively (P＜0.01) after incubation with 100 μg protein/mL oxHDL and oxLDL for 20 h; oxHDL could stimulated the expression of LFA-1 on THP-1 cells, the positive cell rate and mean fluoresence intensity (MFI) increased by 39% and 45% respectively versus control group (P＜0.01) after incubation with cells for 24 h. CONCLUSIONS: By increasing the expression of LFA-1 on monocyte, oxHDL can promote the monocyte-endothelium adhesion; oxHDL also can reduce the membrane fluidity of monocyte, that will limit the returning of monocytes from subendothelium back to the circulation. This suggested that oxHDL may play an important role in atherogenesis.     

MDCC—MSB1培养基上清液中DNA活性的定性研究

将 Ｍ Ｄ Ｃ Ｃ Ｍ Ｓ Ｂ１ ４８ 小时培养物 １０００ｒ／ｍ ｉｎ 离心的上清液分别用 Ｒ Ｎ Ａ 酶、 Ｄ Ｎ Ａ 酶和蛋白酶 Ｋ 处理后，进行体外试验。结果表明，只有 Ｄ Ｎ Ａ 酶处理后的上清液失去了体外抑制 Ｍ Ｄ Ｖ“８１４”增殖的作用。将该上清液 １００００ｒ／ｍ ｉｎ 离心所得的沉淀分别用上述酶处理后进行体内试验。结果表明，只有 Ｄ Ｎ Ａ 酶处理的样品失去了体内促进 Ｍ Ｄ Ｖ 京１ 株致瘤的作用。同时，电泳分析结果证明，该上清液中确实存在 Ｄ Ｎ Ａ。     

中国华东地区猪生殖-呼吸道综合征病毒(S_1株)ORF_(5～7)基因特征研究

本研究对我国华东地区猪生殖和呼吸综合征病毒（ＰＲＲＳＶ）分离株Ｓ１主要结构蛋白基因（ＯＲＦ５～７）进行了ＰＣＲ扩增和ＤＮＡ测序分析。结果表明，长度为１５２０ｂｐＤＮＡ序列含有３个阅读框ＯＲＦｓ，即ＯＲＦ５、ＯＲＦ６和ＯＲＦ７，ＯＲＦ５和ＯＲＦ６及ＯＲＦ６和ＯＲＦ７间有部分碱基重叠，且与欧洲型和美洲型ＰＲＲＳＶ相似。ＯＲＦｓ推导的氨基酸序列与美国ＶＲ２３３２和欧洲ＬＶ毒株同源性分析为９９％～１００％和５９％～７８％。ＯＲＦｓ预测的蛋白质分子量、等电点、糖基化位点、亲水性分布图及跨膜螺旋区与ＶＲ２３３２毒株相似。     

线粒体nad 6基因在油菜种子发育中的特异性表达

以油菜为材料，研究线粒体ｎａｄ６基因在种子发育中的表达。结果表明，ｎａｄ６基因在叶片中的表达很低，在种子发育过程中各阶段均有较高表达，并随种子发育进程而有加强的趋势。推测线粒体ｎａｄ６基因可能除与呼吸作用有关外还有其他功能