Investigation of Clinical Medicine for Treating Cows Endometritis in Baotou Area

In 4 dairy farms located in Baotou area, clinical medicine investigation and drug resistance of pathogenic bacteria were conducted, and then curative effects of different treatment methods were analyzed.Epidemic materials were collected from diary cows infected by endometritis, from which pathogenic bacteria were isolated using conventional microbiology technologies.Broth microdilution method was used to detect the sensitivity and resistance of pathogenic bacteria to antibiotics commonly used in clinic.On this basis, the sensitive drugs were used to treat mastitis or endometritis, the clinical curative effect of traditional treatment group was compared with that of therapeutic test group.The result of traditional treatment group showed 13 antibiotics were used to treat 160 cases of endometritis, florfenicol was most effective, penicillin was most ineffective.The results of therapeutic test group showed that Staphylococcus aureus, Escherichia coli and Streptococcus were the main pathogenic bacteria of endometritis, which were sensitive to gentamicin and cefalotin, etc.The results of statistical analysis showed that the curative effect of therapeutic test group was extremely significantly different from that of traditional treatment group(P<0.01).Sensitive drugs screened by drug sensitive test could be used to target therapy, and obtain satisfied curative effect.Moreover, this approach could avoid antibiotic abuse.     

中国白酒低度化概述

介绍白酒低度化的原因,分析低度化中品质稳定、去浊澄清和保持原风味等问题,并提出对策,以为低度化白酒的发展提供参考。     

规模化养殖场群体动物中草药服用方法的改进及其应用

利用中草药防治动物疾病已经成为养殖业研究的重要课题，但对动物服用中草药的难度较大，该文改进了传统的人工胃管灌服方法的缺陷，采用将中草药与精料共煮后饲喂，方法简便、价格低廉、节省人力，对规模化养殖场群体动物的中草药应用具有良好的推广价值。     

用户行为驱动的网页布局自动调整的研究

逻辑观是困扰当前中国逻辑史研究的根本性问题。两千多年来，逻辑的名称虽几经变化，但对命题形式、推理形式及其有效性的分析，始终是西方逻辑不变的主题。由于中国逻辑史研究是基于西方逻辑的传入而产生的，因此，西方逻辑的逻辑观应当成为中国逻辑史研究的基本遵循。     

基于信号博弈的阳澄湖大闸蟹绿色标签市场应用分析

水产品绿色标签对消费者来说是高质量的信号,对卖家来说则是卖出高价的资本.但是欺诈行为的存在损害了消费者和其他卖家的利益,扰乱了市场秩序.以阳澄湖大闸蟹为例,通过信号博彝理论,对螃蟹市场中关卖双方的消费行为进行博彝分析,得到了精炼贝叶斯Nash均衡.     

绵羊FGF10基因克隆、序列分析及其在毛囊发育中的表达分析

本试验旨在获得中国美利奴羊成纤维细胞生长因子10（fibroblast growth factor 10，FGF10）基因的编码区（CDS）全长序列并进行生物信息学分析，随后对FGF10基因在中国美利奴羊毛囊发育过程中的表达特征进行分析，明确其在中国美利奴羊毛囊发育过程中的表达模式，为进一步研究FGF10 mRNA表达水平与中国美利奴羊毛囊生长发育的表达调控机制奠定理论基础。采用PCR扩增获得中国美利奴羊FGF10基因CDS，并克隆到zero PCR@^TM-Blunt进行测序验证；利用实时荧光定量PCR技术检测FGF10在中国美利奴羊毛囊发育过程中的表达差异。结果表明，绵羊FGF10基因CDS长度为696 bp（序列上传GenBank，获得登录号：MT872422），编码231个氨基酸，与牛和山羊的氨基酸序列同源性达100%，存在1个信号肽和1个跨膜结构域，其为分泌通路信号蛋白；实时荧光定量PCR分析表明，FGF10基因在中国美利奴羊毛囊发育过程中均表达，在毛囊发育第85天表达最高，显著高于其他毛囊发育时期（P<0.05）。本研究获得中国美利奴羊FGF10基因完整的编码区序列和毛囊发育过程中的表达特征，生物信息学分析发现，FGF10基因编码区序列具有物种间的保守性，同时FGF10在绵羊毛囊不同发育阶段的皮肤组织中表达，由此表明，FGF10基因可能在绵羊毛囊的生长发育过程中发挥重要的生物学作用。     

Foliar nematode,Litylenchus crenatae ssp. mccannii,population dynamics in leaves and buds of beech leaf disease‐affected trees in Canada and the US

A foliar nematode, Litylenchus crenatae ssp. mccannii, is associated with beech leaf disease (BLD) symptoms. Information about the types of tissues parasitized and how nematode populations fluctuate in these tissues over time is needed to improve surveys as well as understand the nematodes role in BLD. During this study, the nematode was detected throughout the known range of BLD by researchers at both Canadian and US institutions using a modified pan method to extract nematodes. Monthly collections of symptomatic and asymptomatic leaves during the growing season (May–October), and leaves and buds between growing seasons (November–March), revealed that nematodes were present in all tissue types. Progressively larger numbers of nematodes were detected in symptomatic leaves from Ohio and Ontario, with the greatest detections at the end of the growing season. Smaller numbers of nematodes were detected in asymptomatic leaves from BLD‐infected trees, typically at the end of the growing season. The nematode was detected overwintering in buds and detached leaves. The discovery of small numbers of nematodes in detached leaves at one location before BLD was detected indicates that nematodes may have been present before disease symptoms were expressed. Other nematodes, Plectus and Aphelenchoides spp., were infrequently detected in small numbers. Our findings support the involvement of the nematode in BLD and indicate that symptoms develop only when certain requirements, such as infection of buds, are met. We also found that the nematode can be reliably detected in buds and leaves using the modified pan extraction method.     

大白菜游离小孢子培养技术高效体系的研究

以大白菜吉红82、#534DH 群体及#438 群体为试材，研究了利用细胞破碎仪机械提取小孢子与人工挤压提取小孢子对大白菜游离小孢子活力及提取量的影响。结果表明：机械提取替代人工挤压可在短时间内同时提取多份样品，并可快速调整小孢子的培养浓度。收集40 个适期花蕾于5 mL 离心管内，3 000 r·min-1 破碎10 s，将提取的小孢子定容于25 mL NLN-13 培养基，可确保小孢子浓度在适宜培养范围内，并成功诱导胚胎发生。     

佛山岭南天地旧改项目中开放空间的特色分析

近年来,国内出现了不少基于城市老街区改造为商业街区的项目。通过对佛山岭南天地项目中有代表性的广场、街道、建筑等节点进行调研,选取开放空间的3个特色要素——空间形态和尺度,传统装饰元素,植物和水景布置,探析"新天地"模式中开放空间营造的特色手法,包括保持街巷形态和宜人尺度,增强街区公共属性;保护传统建筑构件,利用传统材料、技术;保留古树,并结合植物和水景布置,形成多层次的景观系统等。