PDA在营造林综合核查工作中的应用探讨

营造林综合核查工作中应用PDA，改进了传统的外业调查方法，提高了工作效率，减少了外业工作强度，具有推广应用价值。     

几种含氮盐对平菇菌丝体生长的影响

在PDA培养基中添加了不同浓度的Ca(NO3)2·4H2O、 KNO3、(NH4)2SO4、 NH4Cl和NH4NO3几种含氮盐,研究其对平菇菌丝体生长的影响.结果表明:未添加任何含氮盐的PDA培养基中菌丝长势最好:日均生长速率最快(15.75 mm/d),满管时间最短(6 d),菌丝粗壮(5 μm),菌丝体干重(0.0800 g).添加含氮盐后,并没有促进菌丝的生长.但添加0.5%的Ca(NO3)2·4H2O、0.3%的KNO3有利于强壮菌丝.添加0.2%和0.5%的 NH4Cl,0.1%、0.4%和0.8%的 NH4NO3则有利于增大菌丝生长的密度或菌丝积累物质.     

PDA在林业调查中的应用初探

在林业调查中，将PDA与导航软件OziExplorer以及相关软件结合，以取代传统的林业调查方法，并对该方法的具体应用和可行性作了探讨，对提高林业调查的效率和精度有着重要意义。     

基于PDA奶牛管理信息服务平台的研究与实现

阐述了运用信息化手段和人工智能决策方法,设计并实现专门为奶农量身定制的基于PDA奶牛管理信息服务平台.实现奶牛饲养管理与疾病防治基本信息检索,根据通径理论对奶牛近交系数进行估算,对近交系数最小、预增产奶量最高的种公牛进行预测,防止奶牛群体近交,达到优良繁育和实现现场移动办公的目的.     

水稻种子真菌种群研究

1999～2001年对不同地区收集水稻种子样品22个,以滤纸保湿法和分离培养法,从水稻种子内部和表面共分离鉴定出13个属、15个种真菌。在这些真菌中,链格孢菌(Alternaria oryzae)、禾谷镰孢菌(Fusarium graminearum)、黑附球菌(Epicoccum neglectum)、枝孢菌(Cladosporium cladosporioidas)为优势种。链格孢菌在两种分离方法中出现数量都高,禾谷镰刀菌、枝孢菌以分离培养法出现数量多,而黑附球菌则以滤纸保湿法出现的数量多。     

Transesophageal Echocardiography as the Sole Guidance for Occlusion of Patent Ductus Arteriosus using a Canine Ductal Occluder in Dogs

Background

Transcatheter occlusion of patent ductus arteriosus (PDA) is usually performed by fluoroscopy alone or together with transesophageal echocardiography (TEE). Transthoracic echocardiography (TTE) guidance has been used for deployment of Amplatz Canine Ductal Occluder (ACDO), but sometimes is limited by suboptimal acoustic windows. Transesophageal echocardiography can overcome such issues and provides higher image resolution at the level of the great vessels.

Objectives

To determine if TEE without fluoroscopy could be used to successfully perform ductal occlusion for the treatment of PDA in dogs.

Animals

Twenty client‐owned dogs with PDA.

Methods

A prospective consecutive case series of PDA occlusion was performed using only TEE guidance. Dogs were positioned in right lateral recumbency and the TEE probe was positioned to visualize the descending aorta, PDA, and pulmonary artery. The guide wire, long introducer sheath, and ACDO were imaged by TEE to direct deployment.

Results

Ductal occlusion was performed successfully without need for fluoroscopy and without complications in 19 dogs. One dog required a second larger ACDO because of embolization of the first device 18 hours after positioning.

Conclusions and Clinical Importance

We have demonstrated that TEE monitoring without concurrent fluoroscopy can guide each step of transcatheter ACDO embolization thereby providing an alternate method of visualization for this procedure. Use of TEE alone can reduce radiation exposure or is an option when fluoroscopy is not available, and, therefore, should be evaluated in a larger case series to better assess procedural failure rates.     

森林景观设计研究综述

合理的森林景观设计可以使森林同时发挥经济、社会和生态效益。文中介绍了各种流派的森林景观设计定义及体系, 如英国主动设计途径、美国视觉管理系统、加拿大不列颠哥伦比亚设计途径的理论和方法等; 系统阐述了英国主动设计途径与美国视觉管理系统2种主要设计途径在西班牙和爱尔兰的应用; 结合我国的审美理念, 提出了建立有中国特色的森林景观设计体系的建议。     

Real-time quantitative PCR based method for the quantification of fungal biomass to discriminate quantitative resistance in barley and wheat genotypes to fusarium head blight

Fusarium head blight (FHB) caused by Fusarium graminearum is one of the devastating diseases of small grain crops, including barley and wheat. Breeding for resistance is one of the best and ecofriendly strategies to manage the FHB. However, the existing methods used for screening genotypes, both under field and greenhouse conditions, often resulted in high experimental error, leading to inconsistent ranking of genotypes over years. In the postgenomic era, precise assessment of resistance is crucial to identify candidate genes. Here, we report a pathogen inoculation procedure and a real-time quantitative polymerase chain reaction (qPCR) based protocol for the quantification and discrimination of quantitative resistance among barley and wheat genotypes to FHB. Using Fusarium specific primer pair Tri6_10, for the trichothecene biosynthetic cluster (Tri6) gene, we successfully quantified the relative fungal biomass in both spikelets and rachis. A qPCR of spikelets and rachis collected on 6 dpi, from inoculated three alternate spikelet regions, discriminated resistance with less experimental error than those based on the proportion of spikelets diseased (PSD) at 9 dpi. This method can be applied for medium to high-throughput barley and wheat breeding programmes to discriminate quantitative resistance among genotypes against FHB.     

基于PDA的生猪溯源管理系统设计与实现

采用软件工程的方法建立基于PDA的生猪溯源管理系统,提供猪肉食品安全的可溯源管理。该系统集猪只养殖信息管理和猪只检疫2种功能为一体,实现对猪只基本信息、饲料使用、兽药使用和检疫情况等的真实记录与追踪溯源。笔者主要对该系统体系结构设计、功能结构设计以及系统实现等关键技术进行了介绍。     

A Solution on Pork Quality Traceability from Farm to Dinner Table in Tianjin City, China

In order to meet government supervision of pork production safety as well as consumer＇s right to know what they buy and protect the public safety of pork food, this study adopts animal identification, intelligent personal digital assistant （PDA） reading and writing, general packet radio service （GPRS）, and other information technologies, proposes a pork tracking and traceability inferstructure based on pork production substrace flow and data flow, designs the metadata structure and related datatbases for farming, slaughtering, and retailing sector based on intensive pig farming and smallhold pig farming, develops three different data-recording systems, and finally establishes a public network platform for the information inquiry in light of ＂the administrative rules on identification and rearing files for animal and poultry＂ in China. The farming process information system supplies early warning for the usage of drugs and feed additives based on data of every individual pig and timely uploading all events data to remote traceability database when pigs are sold; the PDA data collecting system can collect farming events data for pigs fed by farmers and submit to the center database by GPRS; the web-based Tianjin＇s pork traceability platform can integrate all identifications and related pork quality data from farming, slaughtering to marketing by online, and achieve pork tracking from product origin to consumption and tracing in the turnover direction. It is feasible to realize pork quality traceability by identification technologies developed and/or integrated, metadata specifications designed, three data-recording systems deyeloped, and web-inquiring platform established. Some individual technical bottlenecks will be resolved with the development of communication technologies. The full implementation in Tianjin, China, will supply technical support for guaranteeing the quality and safety of pork production and meeting consumer＇s demands.