直喷式柴油机排放性能的试验研究

对由车用6110直喷式柴油机改造的单缸柴油机的排放特性进行了试验研究。结果表明，在大负荷和高速小负荷工况下，微粒的排放量较大；中等转速中等负荷工况下，NOx的排放量较大。     

The Effects of Mx1 Locus on Immunity Tracts Components in Large White×Meishan F2 Offspring

The mouse Myxovirus resistance protein 1 (Mx1) is known to be sufficient to confer resistance to influenza viruses, and genes encoding Myxovirus resistance protein 1 (Mx1) is, therefore, an interesting candidate gene for disease resistance in farm animals. The porcine Mxl gene has already been identified and characterized based on its homology with mouse Mxl; the full-length coding region of the pig Mxl gene spans 2 545 bp (M65087) and is organized into 17 exons compared with the human ortholog mRNA. In this study, the exons 9, 10, 11 and introns 6, 9 of the porcine Mxl gene were cloned and sequenced; two SNPs were identified in exons 9, 10, 11 but none of the SNPs led to an amino acid exchange, and the other eleven variants were detected in introns 6 and 9, respectively. Differences in allele frequency among Meishan, Large White, Tibetan, Tongcheng, Huainan, and Duroc pigs were observed within intron 9, of which an A → G substitution at position 186 was detected as an Msp Ⅰ PCR-restriction fragment length polymorphism (PCR-RFLP). The association analysis using the Large White×Meishan F2 offspring suggested that the Mxl genotype was associated with variation in several immunity traits that are of interest in pig breeding. However, further investigations in more populations are needed to confirm the above concept.     

向日葵叶中绿原酸提取方法的比较研究

采用4水平5因素（乙醇浓度、乙醇用量、回流时间、pH值、浸泡温度）正交试验，优选从向日葵叶中提取绿原酸的最佳提取工艺．结果表明：稀醇法为最佳提取方法；最佳提取工艺为以10倍70％乙醇为浸提液，pH值调至6．0，80℃水浴浸泡，回流3h，所得绿原酸含量最高．     

基于GIS的松树萎蔫病发生格局研究

通过GIS对发病信息的空间定位以及发病林型几何质心的挖掘,对空间点(单元)发病属性数据建立距离矩阵,利用空间自相关性指数研究探询安徽滁州地区松树萎蔫病发生的空间扩散机制,结果表明:松树萎蔫病在安徽滁州和马鞍山地区的发病率从0.18%到46%不等,严重发病的区域主要集中在明光县管店林业总厂及其相邻的鲁山林厂和老嘉山林场,发病率都在40%～46%;其次为马鞍山地区的横山林场及其附近的薛津镇.在安徽滁州地区和马鞍山地区松树萎蔫病的发生和扩散有适宜的生境条件,该地区松材线虫易感植被集聚分布的特点使松树萎蔫病持续发生和蔓延成为可能.空间自相关性的分析发现,松树萎蔫病在该区域内发病的Moran's Ⅰ的空间自相关指数高达74.86%(0.05水平检验),推断在安徽滁州和马鞍山地区1316个以林型为发病单元的发病点的分布格局为高度聚集型,同时也说明松树萎蔫病在安徽滁州和马鞍山地区的发生具有一定强度的传染性.     

柑桔黄龙病的常规PCR及荧光定量PCR检测

【目的】为柑桔黄龙病的早期诊断和寄主体内病原菌的动态监测提供一种稳定、可靠的检验检疫技术。【方法】利用亚洲韧皮杆菌核糖体蛋白基因rplJ/rplL设计了2对PCR引物CQULA03F/CQULA03R、CQULA04F/CQULA04R和1条TaqMan探针CQULAP1，以此为基础建立了常规PCR和TaqMan探针法、SYBR Green I荧光染料法两种荧光定量PCR（共3种）反应体系；确定了3种体系各自的检测灵敏度、特异性和准确性，据此对3种体系进行了比较；从2004年7月到2005年5月还利用常规PCR和TaqMan探针荧光定量PCR体系完成了对柑桔黄龙病病原菌在寄主体内的周年变化的动态监测。【结果】两种荧光定量PCR方法的灵敏度比常规PCR高出至少2～3个数量级，而TaqMan探针法由于使用了杂交探针，其特异性尤其可靠，另外两种定量PCR较小的产物片段使得它们具有更好的稳定性，加上荧光定量PCR方法本身受污染可能性小、操作简便等固有优势，使得前者更适合于柑桔黄龙病的检测。【结论】本研究建立的2种荧光定量PCR方法可以为柑桔黄龙病的病害早期诊断以及柑桔黄龙病病原菌近缘种的甄别提供准确、灵敏、快速的检验检疫技术。     

SYBR Green I实时荧光PCR鉴定检测转基因油菜RT73品系

根据转基因油菜RT73品系转入的外源基因质粒图谱,设计品系鉴定的特异性引物,进行SYBR!Green I实时PCR检测,建立转基因油菜品系鉴定的SYBR Green I实时PCR方法。同时针对油菜FatA基因设计出内源基因。结果表明：应用实时PCR技术,利用SYBR Green I染料能选择性结合双链DNA的特点,可检测到RT73品系特异性靶序列扩增所产生的荧光信号,且通过溶解曲线确定其熔点值为80.2（±0.5）。SYBR Green I实时PCR能通过溶解曲线有效地区分特异性产物、非特异性产物以及引物二聚体,是鉴定转基因油菜RT73品系的新方法。     

如何提高吉林省玉米的市场竞争力

分析了国内外玉米市场的现状和吉林省玉米品质下降的原因以及吉林省玉米在国内外市场上竞争力低的原因,指出吉林省玉米生产加工中存在的主要问题,提出几点解决方法。     

豌豆光系统I的分布及其DOC-PAGE分离特性研究

以豌豆(Pisum sativumL.)苗叶片为试材,提取其类囊体膜并利用不同增溶剂研究PSI蛋白复合物的分布以及不同凝胶浓度的DOC-PAGE分离特性。结果表明:6%的分离胶对PSI具有较好的分离特性;低浓度的毛地黄皂苷能够增溶间质类囊体上的PSI蛋白复合物,并能够获得大量的含有PSI的类囊体膜碎片。高浓度的毛地黄皂苷能够获得全部的PSI复合物;Tween-20能够分离不同区域的类囊体膜,结合毛地黄皂苷处理均能够获得PSI复合物。证明了PSI异质性的存在。     

主成分分析法在昆虫目级阶元分类上的应用

根据昆虫图象,对半翅目、鳞翅目、鞘翅目的28种昆虫提取的形状参数、叶状性、球状性等7项数学形态特征进行了主成分分析,利用主成分分析法所得出的结果与传统分类方法相比较,结果表明:半翅目中除黑哎猎蝽独立外,基本上归属于鞘翅目中,鳞翅目中除棉铃虫独立外,其他昆虫种类仍在一类。这说明主成分分析的分类结果与传统分类有较大的相似性,同时也揭示了传统的分类结果可能存在不足,利用数学形态特征和先进的数学方法对昆虫分类进行研究具有重要的意义。     

Molecular characterization of Korean rabies virus isolates

The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the N and G genes, the Korean RABV isolates were confirmed as genotype I of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts.