Evaluation of Associative Effect of Whole Corn Silage and Millet Straw by Gas Production Technique in vitro

This research adopted fermentation gas production method in vitro to evaluate the associative effect of whole corn silage (WCS) and millet straw (MS).The WCS was mixed with MS in the proportion of 100:0,80:20,60:40,50:50, 40:60,20:80 and 0:100 with 3 replicates per group.The gas production,pH,ammonia nitrogen (NH₃-N), volatile fatty acids (VFA), microbial crude protein (MCP) and dry matter loss (DML) for 24,48 and 72 h of varying forage combinations were analyzed.Single factor associative effects index (SFAEI) and multiply factors associative effects index (MFAEI) of different combinations were calculated. The results showed as follows:There were significant differences of gas production among different combinations (P<0.05),and the best combination was the WCS80 group. With creasing of the content of MS,DML decreased,but the DML of WCS80 and WCS100 were similar (P>0.05). The MCP concentration of WCS100 and WCS80 was extremely significantly or significantly higher than other groups (P<0.05;P<0.01). The VFA concentration showed significant differences among different combinations (P<0.05).Among the different combinations of WCS and MS,the MFAEI of WCS80 was the largest,and that were 0.5517,0.5094 and 0.9860 at 24,48 and 72 h,respectively. The best combination of WCS and MS was 80:20.     

猪肺疫EO630菌株变异情况的观察

猪肺疫EO630弱毒菌株,是成都兽医药械厂用猪源B型多杀性巴氏杆菌强毒菌株,通过含有海鸥牌洗涤剂的培养基中连续传代选育而成。多年以来,该菌株被广泛用作猪肺疫弱毒冻干疫苗及其联苗的生产菌株,为我国预防猪肺疫疫病作出了重要的贡献。从70年代初开始,我厂一直使用成都药?..     

Cloning and Expression of CMKLR1 Gene and Its Correlation with Intramuscular Fat in Goat (Capra hirus)

In order to enrich basic date in goat (Capra hirus) CMKLR1 gene and investigate the correlation between CMKLR1 gene expression and intramuscular fat (IMF) content in muscles, the CMKLR1 gene was cloned from the goat of subcutaneous adipose tissue by RT-PCR, characterized by bioinformatics methods. The expression profiles of CMKLR1 gene of goat in various tissues were constructed liver was benchmark, the GAPDH (GenBank accession No.: AJ431207.1) as a reference gene.Then, the correlation between CMKLR1 mRNA expression and IMF content in muscles was analyzed. The results showed that the CMKLR1 gene CDS of goat (GenBank accession No.: KT165374) was 1 089 bp. Real-time PCR indicated that CMKLR1 was widely expressed in various tissues, including heart, liver, spleen, lung, kidney, adipose, longissimus dorsi, biceps femoris and triceps brachii, and was the highest in lung (P< 0.05). Similar expression variation trend of CMKLR1 was observed between the muscles from 1 to 3 and 24 months old goat, and was the highest in biceps femoris. Reversely, in 8 to 10 months old CMKLR1 was the highest in triceps brachii. The IMF of longissimus dorsi from 24 months old was the highest. Correlation analysis demonstrated that different correlations were observed between expression of CMKLR1 mRNA and IMF content in longissimus dorsi, biceps femoris, and triceps brachii in goat. The CMKLR1 gene did not participate in the deposition of IMF in goats. The research built the theoretical basis for further studies about the CMKLR1 gene.     

哈密大枣枣树落头的管理技术

为了控制树体高度,便于枣树生产管理,降低因输送营养而造成的能量消耗,实现增产增收的目的,2014年春季,我们在哈密大枣丰产栽培示范园中,选取了7年生、株行距配置为3米×4米的3.33公顷大枣园进行落头技术示范,现将技术环节总结如下:一、技术实施1.修剪落头时间为保证在枣树休眠期修剪落头,2014年落头修剪的时间为3月15~20日。2.降低树体高度为了便于今后管理,所留树体高度控制在1.8~     

《技术与市场》杂志征集论文启事

《技术与市场》杂志是经国家科技部和国家新闻出版总署批准、国内外公开发行的综合性科技类期刊，由四川省科技厅主管。四川省科技信息研究所主办，月刊。本刊国内统一刊号：CN51—1450／T,国际标准刊号ISSN 1006—8554，邮发代号：62—125。本刊被《中国学术期刊（光盘版）》、《万方数据——数字化期刊群》、《中文科技期刊数据库》全文收录。     

TMR技术在奶牛场应用效果分析

利用陕西省DHI中心提供的综合分析报告，对影响奶牛产奶量和牛奶质量较大的几项指标进行了统计分析，结果表明：TMR技术对提高乳脂率、产奶量，降低体细胞数，有明显的积极作用，     

柿树密植园早期丰产栽培技术

山东省枣庄市山亭区徐庄镇柿树密植园（株行距为2m×3m或2．0m×2．5m），2009年经实产测定，获得了4年生幼树667m。产1887．6kg的高产记录。其主要栽培管理技术如下：