Genetic bases of source-,sink-,and yield-related traits revealed by genome-wide association study in Xian rice

The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.     

信阳地方文献与信阳地方文化的关系

信阳地方文献与信阳地方文化之间有着十分紧密的关系。独具特色的信阳文化是信阳地方文献产生的基础。同时,信阳地方文献对信阳地方文化的发展起着十分重要的作用:信阳地方文献推动了信阳地方物质文化的发展;信阳地方文献促进了信阳地方精神文化的建设。     

茶树凋落叶浸提液对菘蓝生理生化的化感效应

为研究茶树凋落叶浸提液对菘蓝生长与生理生化的化感效应,以模拟自然条件下雨雾淋溶方式,采用不同浓度的茶树凋落叶浸提液(CK:0 mg·mL^-1、T1:6.25 mg·mL^-1、T2:12.5 mg·mL^-1、T3:25 mg·mL^-1 和T4:50 mg·mL^-1)处理菘蓝,测定其生长、抗氧化酶活性及其基因表达量、细胞膜损伤率以及丙二醛(MDA)、过氧化氢(H₂O₂)、渗透调节物质和次生代谢物含量的变化。结果表明,茶树凋落叶浸提液对菘蓝生长表现出“低促高抑”的浓度效应,与CK相比,T1对菘蓝生长具有一定的促进作用,而浸提液浓度超过菘蓝的耐受阈值时,会对其生长产生不利的影响。随着浸提液浓度的升高,可溶性糖和可溶性蛋白含量呈先上升后下降的趋势,而脯氨酸含量则持续增加。与CK相比,T1的MDA含量、细胞膜损伤率和H₂O含量差异不显著,而T3、T4则显著升高。茶树凋落叶浸提液对抗氧化酶活性及其基因表达有不同的影响,T1的过氧化物酶(POD)与抗坏血酸氧化物酶(APX)活性最高,T2的过氧化氢酶(CAT)活性最高,而T3、T4的4种抗氧化酶活性均显著低于CK。T1的Pod、Cat、Apx基因表达量最高,而T4则抑制了抗氧化酶基因的表达。此外,POD活性与其基因表达量呈显著正相关,而超氧化物歧化酶(SOD)、CAT和APX活性与其基因表达量的相关性不显著。不同浓度的茶树凋落叶浸提液对于菘蓝次生代谢物质积累的影响存在显著差异,低浓度茶树凋落叶浸提液对菘蓝生长、靛蓝和靛玉红含量积累有促进作用,而高浓度茶树凋落叶浸提液对总黄酮含量积累有一定的促进作用。本研究结果可为幼龄茶园中茶树-菘蓝复合种植提供理论参考。     

有机质浸染砂水泥土的力学特性及本构关系

为研究海南省海湾地区分布的有机质浸染砂水泥土的力学特性,该文首先对有机质浸染砂水泥土和标准砂水泥土进行无侧限抗压强度对比试验(采用配合比均为熟石灰掺入比7.5%、水泥掺入比20%、水灰比0.45),定量分析养护龄期对其无侧限抗压强度及试样破坏形式的影响。然后对有机质浸染砂水泥土进行单轴抗压试验,获得了水泥土材料的应力-应变全过程曲线、刚度变化规律以及改进邓肯-张本构模型。结果表明:1)有机质浸染砂水泥土试样的破坏类型为塑性剪切破坏和脆性剪切破坏;2)有机质浸染砂水泥土抗压强度随着养护龄期的增加基本呈指数形式增长,但在养护龄期14 d后,增长速度逐渐降低并趋于稳定;3)随着养护龄期增长,水泥土刚度增加。在加载初期,水泥土切线模量随着轴向应变增加而增大,呈现刚度硬化现象;4)基于单轴抗压试验得到应力-应变全过程曲线,可分为2个阶段:塑性阶段、软化阶段;5)通过对应力-应变全曲线的描述,得到了修正的邓肯-张模型,确定模型参数后,与实测数据对比发现,该修正模型可以模拟有机质浸染砂水泥土的应力-应变关系。     

2016—2018年度国家区试小麦品种品质性状灰色关联度分析与评价

为研究近年来参试小麦品系品质状况并筛选优质品种,对2016—2018年参加国家冬麦区黄淮南片水地组品种区域试验的20个小麦新品系的5个性状进行灰色关联度分析。结果表明,各指标与品质综合评价值的关联度(r_i)和权重(r_j)2年均表现为容重>湿面筋含量/吸水率>蛋白质含量>稳定时间。2017年5个品种综合评价值高于对照,2018年10个品种综合评价值高于对照。‘郑科麦136’连续2年综合评价值列首位。无论是否考虑各指标对品质影响的权重,‘漯麦26’品质2年间差异最小。灰色关联度分析法在品质性状评价中具有一定的科学性,但受评价指标较少、各指标赋权合理性的影响,有一些局限性。在品种应用时应根据最终用途进行细化评价,不同类型的品质参数应分别做灰色关联度分析,以保证分析结果准确。     

Early interspecific dynamics,dry matter production and nitrogen use in Kernza (Thinopyrum intermedium) – alfalfa (Medicago sativa L.) mixed intercropping

ABSTRACT

The global interest in growing perennial grain crops such as intermediate wheatgrass (Thinopyrum intermedium) (Kernza) for production of food and feed is increasing. Intercropping Kernza with legumes may be a sustainable way of supplying nitrogen to soil and associated intercrop. We determined the competitive interactions between intercropped Kernza (K) and alfalfa (Medicago sativa L.) (A) under three inorganic nitrogen (N) rates N0, N1, N2 (0, 200, 400 kg ha^?1) and five species relative frequencies (SRF) (100%K:0%A, 75%K:25%A, 50%K:50%A, 25%K:75%A and 0% K:100%A) in mixed intercrops (MI) in a greenhouse pot experiment. After 11 weeks of growth. Kernza dry matter yield (DM) and N accumulated (NACC) were low, but alfalfa DM and NACC high at 0 kg N ha^?1. 200 and 400 kg N ha^?1 fertiliser application increased the competitive ability (CA) of Kernza and reduced the CA of alfalfa. SRF had large impacts on alfalfa DM, NACC and NFIX only at 0 kg N ha^?1 fertiliser, and insignificant impacts on Kernza at all N fertiliser levels, indicating that adjustment of SRF may not be an effective way to modulate the interspecific competition of Kernza. Further research on the other factors that influence the interspecific competition are warranted.     

鹰嘴豆短肽的分步酶解法制备及其营养价值评价

以鹰嘴豆蛋白为原料,建立复合酶分步酶解法制备鹰嘴豆短肽的工艺。在鹰嘴豆蛋白碱性蛋白酶Alcalase水解的基础上,进一步采用中性蛋白酶和风味蛋白酶Flavourzyme继续水解鹰嘴豆蛋白碱性蛋白酶Alcalase酶解物,并对各影响因素进行研究,建立短肽得率与各影响因素的回归模型,利用高效液相色谱法和氨基酸自动分析仪等测定鹰嘴豆短肽的相对分子质量、氨基酸组成、一般营养成分,评价鹰嘴豆短肽的营养价值。结果表明,中性蛋白酶和风味蛋白酶Flavourzyme制备鹰嘴豆短肽的最佳工艺参数为:复合酶添加量5 678 U/g,pH 7.0,水解时间216 min,水解温度55℃,在此条件下,短肽得率为63.79%,与碱性蛋白酶Alcalase单独酶解相比明显提高,水解度为26.74%;大部分水解产物的相对分子质量低于1 000、脂肪含量低,蛋白质、必需氨基酸等含量丰富,与FAO/WHO推荐的成人需求量模式相比,其第一限制氨基酸是蛋氨酸和半胱氨酸,与学龄儿童需求量模式相比,其第一限制氨基酸是苏氨酸,氨基酸分分别高达138.18和103.25;必需氨基酸与非必需氨基酸比值(EAA/NEAA)为0.73,接近FAO/WHO参考标准值0.6。该研究为进一步开发利用和工业化生产鹰嘴豆短肽奠定了基础。     

塔里木河上游棉区不同类型盐土阳离子交换量分布特征及影响因素

【目的】研究塔里木河上游棉区不同类型盐土CEC的分布状况及影响因素。 了解不同类型盐土土壤特性及肥力状况,为新疆南疆干旱区盐土改良利用提供理论依据。【方法】以阿拉尔绿洲草甸盐土(MeS)、沼泽盐土(MaS)、残余盐土(ReS)、洪积盐土(PrS)、棕漠林盐土(DeS)5种典型盐土为对象,研究该流域不同类型盐土阳离子交换量(CEC)的剖面分布规律,分析盐土CEC的影响因素,并利用灰色关联法对盐土CEC与土壤理化性质之间的关系进行了量化。【结果】该区盐土CEC在2.02～25.54 cmol/kg,平均值为12.39 cmol/kg,CEC随剖面深度的变化规律各类型盐土有所不同,不同类型盐土CEC大小依次为MeS> ReS > DeS > PrS > MaS。粘粒是干旱区盐土CEC的主要来源;pH、CaCO₃、盐基总量(TEB)和碱化度(BSP)与盐土CEC呈极显著正相关,是盐土CEC的主要影响因素,其中CaCO₃对盐土CEC的贡献不容忽视;盐土有机质(SOM)含量极低,对盐土CEC未表现出显著性影响;交换性钙饱和度(CaSP)、交换性镁饱和度(MgSP)及交换性钾饱和度(KSP)对盐土CEC有显著的抑制作用。【结论】塔里木河上游棉区不同类型盐土CEC有显著差异,不同类型盐土保肥、供肥及缓冲性能不同。pH、CaCO₃、TEB和ESP是CEC主要贡献因子,SOM对盐土CEC无显著的影响。     

山西云顶山亚高山草甸优势种种间关系分析

为揭示山西云顶山亚高山草甸植物群落优势种的种间关系,本研究通过种间联结性和相关性测定,对其植物群落13个优势种78个种对的种间关系进行研究。结果表明:13个优势种间大部分种对的种间关联不显著,种间联结不紧密,说明山西云顶山亚高山草甸植物群落结构不很稳定,存在一定程度的生态退化风险;多数原生优势种在群落中占据主要位置,同时也出现少数非原生优势种优势明显,有逐渐取代典型原生优势种的逆行演替趋势,这是由于人类频繁的旅游扰动所致。因此,应当合理的控制旅游开发强度与游客数量,保持亚高山草甸生态系统的稳定与自我维持,以持续高效的维持云顶山亚高山草甸的经济及生态价值。     

Segment-based coancestry,additive relationship and genetic variance within and between the Norwegian and the Swedish Fjord horse populations

ABSTRACT

The Fjord horse originates from Norway but forms a global population due to several small populations in foreign countries. There exists no information about the additive relationship and the genetic variance between these subpopulations. By collecting blood samples from Norwegian and Swedish Fjord horses, a sample of 311 Norwegian and 102 Swedish horses gave 485,918 SNPs available for analysis. Their inbreeding coefficients were calculated and compared to the pairwise coancestry and the shared genomic segments. The effective population size was almost similar with the two methods in the Norwegian Fjord horse population (63 and 71), but very different in the Swedish population (269 and 1136) and unprecise due to a much smaller number of observations. The study showed that coancestry from shared genomic segments can be used to estimate additive genetic relationship and genetic variation within and between the global populations of the Fjord horse.