甜瓜持绿蛋白基因家族的全基因组鉴定及进化分析

持绿蛋白在调节植物叶绿素降解和衰老过程中起到非常重要的作用,利用公布的甜瓜基因组数据,使用生物信息学方法对甜瓜全基因组SGR基因的结构、系统进化关系、保守基序及表达谱进行分析。结果表明：甜瓜中共有4个SGR类型基因,命名为CmSGR01~CmSGR04,其蛋白质在167~257 aa之间,通过系统进化树分析,发现甜瓜与黄瓜属同一科,它们的SGR聚在一起,说明相对其他物种,二者的SGR蛋白在其他物种分化后进化较小;MEME软件分析发现了SGR因含有5个保守的基序。通过甜瓜SGRs基因家族的系统分析,为进一步阐明甜瓜中SGR蛋白结构及功能提供理论基础和有价值的资料。     

Experiential legacies of early-life dietary polyunsaturated fatty acid content on juvenile Walleye: Potential impacts from climate change

Climate-induced shifts in plankton blooms may alter fish recruitment by affecting the fatty acid composition of early-life diets and corresponding performance. Early-life nutrition may immediately affect survival but may also have a lingering influence on size and growth via experiential legacies. We explored the short- and longer-term performance consequences of different concentrations of polyunsaturated fatty acids (PUFA) for juvenile Walleye (Sander vitreus, Mitchill 1818). For the first 10 days of feeding, juveniles were provided Artemia enriched with: oleic acid (low PUFA), high docosahexaenoic acid and high eicosapentaenoic acid (high PUFA), or high PUFA and a form of vitamin E (high PUFA + E). After 10 days, all fish were fed a high-quality diet and reared for an additional 27 days. Juveniles fed either high PUFA diet were 1.15-fold larger (PUFA mean ± SD = 20.0 ± 3.3 mg; PUFA + E = 19.8 ± 3.3 mg) than those fed the low PUFA (17.3 ± 2.8 mg) diet after 10 days of feeding. After 27 days, juveniles initially fed the high PUFA diet were still 1.10- to 1.20-fold larger (PUFA = 407.0 ± 61.6 mg; PUFA + E = 422.7 ± 58.7 mg) than those initially fed the low PUFA diet (356.5.0 ± 39.5 mg). Our findings demonstrate that fatty acid composition of juvenile Walleye diets has immediate and lingering size effects. As changes in climate continue to alter lower trophic levels, fish management and conservation may need to consider short- and long-term effects of temporal or spatial differences in early-life diet quality.