全文获取类型
收费全文 | 21198篇 |
免费 | 1306篇 |
国内免费 | 1665篇 |
专业分类
林业 | 1762篇 |
农学 | 1693篇 |
基础科学 | 644篇 |
1911篇 | |
综合类 | 7442篇 |
农作物 | 2373篇 |
水产渔业 | 1362篇 |
畜牧兽医 | 4315篇 |
园艺 | 1868篇 |
植物保护 | 799篇 |
出版年
2024年 | 142篇 |
2023年 | 353篇 |
2022年 | 607篇 |
2021年 | 844篇 |
2020年 | 751篇 |
2019年 | 939篇 |
2018年 | 736篇 |
2017年 | 790篇 |
2016年 | 1145篇 |
2015年 | 1279篇 |
2014年 | 1482篇 |
2013年 | 1522篇 |
2012年 | 1748篇 |
2011年 | 1451篇 |
2010年 | 1162篇 |
2009年 | 1171篇 |
2008年 | 936篇 |
2007年 | 1040篇 |
2006年 | 1100篇 |
2005年 | 837篇 |
2004年 | 572篇 |
2003年 | 439篇 |
2002年 | 411篇 |
2001年 | 367篇 |
2000年 | 394篇 |
1999年 | 287篇 |
1998年 | 271篇 |
1997年 | 175篇 |
1996年 | 172篇 |
1995年 | 146篇 |
1994年 | 159篇 |
1993年 | 116篇 |
1992年 | 112篇 |
1991年 | 98篇 |
1990年 | 90篇 |
1989年 | 81篇 |
1988年 | 49篇 |
1987年 | 31篇 |
1986年 | 17篇 |
1985年 | 32篇 |
1984年 | 20篇 |
1983年 | 11篇 |
1982年 | 15篇 |
1981年 | 25篇 |
1980年 | 19篇 |
1979年 | 8篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1956年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 156 毫秒
991.
本文阐述了中华鳖红底板病的流行病学,病原菌的分离、培养、生化鉴定和菌体DNAGC mol%的测定以及毒性试验等,确定了致病菌为气单胞菌属;通过药敏试验及生产实践,提出了有效的防治措施。 相似文献
992.
利用常规肌肉营养测试方法测定分析5尾中华倒刺鲃(Spinibarbus sinensis)的肌肉营养成分。结果表明,中华倒刺鲃肌肉(鲜样)中粗蛋白质量分数19.22%,粗脂肪质量分数1.96%,水分质量分数77.0%,灰分质量分数1.62%,碳水化合物质量分数0.18%。肌肉中含有17种氨基酸,总量为73.61%(质量分数,干样),其中7种人体必需氨基酸(不包括色氨酸)总量是32.26%,占氨基酸总量的43.83%;其必需氨基酸的构成比例基本符合FAO/WHO的标准。中华倒刺鲃的限制性氨基酸为(蛋氨酸 胱氨酸)、缬氨酸和(苯丙氨酸 酪氨酸),必需氨基酸指数(EAAI)为71.34,4种鲜味氨基酸总质量分数为24.22%(干样)。脂肪酸中EPA与DHA质量分数分别为0.87%、3.08%,比其他经济鱼类均高,微量元素比值合理,表明中华倒刺鲃有较高的食用价值与保健作用。 相似文献
993.
Nine isonitrogenous (35% crude protein approximately) and isocaloric (18.37 kJ g?1) experimental diets (RLL20–BCFL40) were formulated with either raw or treated (inoculated with fish intestinal bacteria) Leucaena leucocephala leaf meal at 20%, 30% and 40% levels replacing other ingredients partially from a fish meal based reference diet (RD). Two specific strains of fish intestinal bacteria, Bacillus subtilis (isolated from Cyprinus carpio) and B. circulans (isolated from Oreochromis mossambicus) having extracellular cellulolytic and amylolytic activities, were used to inoculate Leucaena leaf meal for 15 days at 37°C. The crude fibre, cellulose and hemicellulose contents and the antinutritional factors, tannin, phytic acid and mimosine in the leaf meal decreased due to inoculation. However, free amino acids and fatty acids increased in the treated leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a RD. Both the inclusion level and type of Leucaena leaf meal in diets significantly affected the growth performance of rohu. Fish fed diets containing inoculated Leucaena leaf meal performed better in comparison with those with the RD. On the basis of growth response, feed conversion ratio, protein efficiency ratio and apparent net protein utilization, diet formulated with 30%Leucaena leaf meal inoculated with B. circulans resulted in the best performance of rohu fingerlings followed by diet with 40%B. subtilis inoculated Leucaena leaf meal. The apparent protein digestibility (APD) was better in fish fed diets containing B. circulans inoculated leaf meal. An increasing level of raw Leucaena leaf meal was associated with a decrease in the carcass protein content of rohu fingerlings. The activity of α‐amylase increased with the increasing level of treated leaf meal in diets. Cellulase activity increased with increasing level of inclusion of raw leaf meal, and was comparatively lower in fish fed diets with treated leaf meal. Activities of protease and lipase were higher in fish fed the RD. The results showed that it is possible to incorporate Leucaena leaf meal inoculated with enzyme‐producing fish intestinal bacteria in carp diets up to 40% level of inclusion. 相似文献
994.
The effect of aflatoxin treatment and/or feeding of a high level of α‐tocopherol on immune response and disease resistance was investigated in Indian major carp, Labeo rohita. Group A served as a healthy control, group B was treated with aflatoxin, group C was fed a high level of α‐tocopherol whereas group D was exposed both to aflatoxin and a high level of dietary α‐tocopherol for 60 days. Aflatoxin B1 (AFB1) was injected once intraperitoneally into fish on the first day of the experiment (groups B & D). High levels of DL‐α‐tocopherol (1000 mg kg–1 feed) were provided to healthy as well as AFB1‐treated immunocompromised fish for 60 days (groups C & D). At the end of the experiment blood samples were assayed for changes in nonspecific immunity and humoral protein levels. Disease resistance against two common bacterial pathogens viz., Aeromonas hydrophila and Edwardsiella tarda were evaluated in all groups. Significant (P < 0.05) suppression of specific immunity as measured through haemagglutination (HA) titre against sheep red blood cells (SRBCs) as well as bacterial (formalin‐killed E. tarda) agglutination titre; nonspecific resistance factors viz., globulin level, serum bactericidal and lysozyme activities, neutrophil activities, and disease resistance against two bacterial pathogens only in aflatoxin‐treated fish with respect to the control group, clearly indicated the immunosuppressive nature of aflatoxin. Feeding of a high level of α‐tocopherol to AFB1‐treated immunocompromised fish significantly (P < 0.05) raised specific immunity, nonspecific resistance factors and disease resistance capacity when compared with aflatoxin‐exposed fish. Disease resistance and enhancement of immune status through feeding of high levels of α‐tocopherol to healthy as well as AFB1‐treated immunocompromised fish confirmed the potential of α‐tocopherol in carp feed for prevention of disease and for combating natural/environmental immunosuppressants. 相似文献
995.
中华鲟小水体养殖试验初报 总被引:4,自引:0,他引:4
在7.2m^3水泥池中,采和人工配制饲料对中华鲟(Acipenser sinensis)进行养殖试验,通过4个阶段180d的养殖,鱼尾均增重1817.5g,日均增重分别为8.5,8.9,9.2,12.3,饲料系数为1.96,2.12,2.33,2.21。中华鲟在水水温处于8-29,1℃时均可摄食生长,利用人工配制养殖中华鲟的前景乐观。 相似文献
996.
Stefano Peruzzi Anne Kettunen Raul Primicerio & Goran Kauri 《Aquaculture Research》2007,38(9):926-932
The effects of thermal treatments on induction of triploidy in Atlantic cod have been investigated. Cold shock [−1.7±0.1°C at 20 min post fertilization (PF) for 2 h] was based on a previously developed protocol, and heat shocks, below the lethal threshold of 24°C, were at 16, 18 or 20°C applied 20, 30 or 40 min PF for 20 min. Cold shock did not affect larval survival and was ineffective for producing triploids (range 0–4%). A heat shock of 20°C at 20 min PF generated the highest percentages (range 66–100%) of triploid larvae at hatching, with survival ranging from 10% to 20% relative to the controls. Lower heat shock temperatures or delayed shocks increased survival but decreased the number of triploids, providing no net gain in triploid yield (range 1–9%). Heat shocks applied later than 20 min PF produced 2–4% tetraploid larvae at hatching. A thermal shock of 20°C initiated at 20 min PF and lasting 20 min proved to be the most generally efficient treatment for induction of triploidy in Atlantic cod. 相似文献
997.
998.
Rahmi?Ayd?nEmail author Kenan?K?prücü Mustafa?D?rücü Sibel??im?ek?K?prücü Murat?Pala 《Aquaculture International》2005,13(5):451-458
In this study, the toxic effects on the embryos and larvae of the common carp were used as a model to investigate the synthetic pyrethroid pesticide, cypermethrin, which contaminates aquatic ecosystems. Data obtained from the cypermethrin acute toxicity tests were evaluated using the Probit Analysis Statistical Method. The control and eight test experiments were repeated five times. The number of dead embryos significantly increased in response to cypermethrin concentrations 0.0001, 0.001, 0.01, 0.1, 1, 2, 4 and 8 μg l−1 (p<0.05 for each case). The 48 h LC50 value (with 95% confidence limits) of cypermethrin for common carp embryos was estimated at 0.909 (0.256–5.074) μg l−1. Dose–response decreases in hatching success were recorded as 87.4, 85.0, 80.2, 71.4, 56.3, 48.6, 38.8 and 23.5%, respectively. The lowest concentration of cypermethrin (0.0001 μg l−1) produced a significant increase in the number of dead larvae compared to the control group (p<0.05). The number of dead larvae significantly increased with increasing cypermethrin concentrations exposed for 1–96 h (p<0.05). The highest concentration (8 μg l−1) showed the highest larvae mortality. The 96 h LC50 value (with 95% confidence limits) of cypermethrin for common carp larvae was estimated at 0.809 (0.530–1.308) μg l−1. The results of the study suggest that low levels of cypermethrin in the aquatic environment may have a significant effect on the reproduction and development of carp. 相似文献
999.
The current treatment for amoebic gill disease (AGD)-affected Atlantic salmon involves bathing sea-caged fish in fresh water, often sourced from local dams, for 3-4 h. In both a small-scale laboratory and an on-farm field experiment, the effects of water hardness on the efficacy of freshwater bathing were assessed. Results showed that soft fresh water (19.3-37.4 mg L(-1) CaCO3), whether it be naturally soft city mains water or artificially softened dam water, was more efficacious at alleviating AGD in affected fish than hard fresh water (173-236.3 mg L(-1) CaCO3). Soft freshwater bathing significantly reduced viable gill amoebae numbers (from 73.9 to 40.9% of total count) and significantly alleviated gill pathology, both gross and histological. Following bathing, gross gill pathological scores of soft freshwater bathed fish lagged 2 weeks behind hard freshwater bathed fish. Significant gill lesion fragmentation, and shedding of lesion-associated hyperplastic tissue, was accompanied by a significant reduction in AGD-affected gill filaments in soft freshwater bathed fish. Furthermore, soft freshwater bathing alleviated the blood plasma electrolyte imbalance seen in control (sea water) and hard freshwater bathed fish. This study showed that the use of soft fresh water for bathing AGD-affected Atlantic salmon could be an improvement to the current method of treatment. Not only does it reduce gill amoeba numbers, but also, it is of a therapeutic advantage with the potential to reduce bathing frequency. 相似文献
1000.