兔病毒性出血症病毒高免血清的制备与应用效果

以兔病毒性出血症病毒组织灭活疫苗免疫家兔，经五次免疫之后，抗体效价可达12log2以上，采集兔血清，以此治疗攻毒兔，结果大部分兔都存活，临床应用效果表明：以此血清治疗自然感染兔病毒性出血症病毒的发病兔，治愈率达80％以上。     

瞿麦治疗仔猪黄白痢的效果比较

通过用瞿麦单味中药与庆大、链霉素抗生素治疗仔猪黄白痢的效果比较,表明中药瞿麦的治疗效果较好,治愈率达95%以上,明显高于庆大霉素、链霉素等西药的疗效(P＜0.01),是临床治疗黄白痢较理想的中药药剂.     

传染性法氏囊病疫苗免疫母鸡后子代雏鸡外周血液的B细胞和免疫球蛋白含量变化

应用免疫SPA菌体花环法和间接ELISE法对传染性法氏囊病（IBD）疫苗免疫母鸡后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量进行了动态研究。结果发现：IBD疫苗免疫后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量均不同程度地高于未免疫的相应对照雏鸡，表明IBD疫苗免疫母鸡后，其子代雏鸡外周血液的体液免疫功能明显增强。而传染性法氏囊病超强毒株攻击子代雏鸡后，未免疫子代雏鸡外周血液的上述各项指标均明显低于疫苗免疫的子代雏鸡，这与IBDV感染雏鸡后，其体液免疫中枢器官法氏囊严重损害，淋巴细胞变性坏死等有关，也是导致感染雏鸡免疫抑制的基础。     

辽宁西部半干旱地区油松纯林改造的研究

喀左县位于辽宁西部地区，现存大面积油松人工纯林，不仅改善地力和水土保持效益差，而且火险性强、虫害蔓延成灾、生长早期衰退。为改善其林分生态条件，提高林木生长量和综合效益，在中三家镇对25年生油松人工纯林进行改造试验。结果表明，选择山杏、刺槐、沙棘等乔、灌木树种，利用林间空地、林缘隙地、侵蚀沟壑和疏林地造林，在大面积油松纯林连片地区进行窄带状或块状砍伐后造林，5年后效益显著，林分相对湿度提高了2．2％，土壤有机质含量提高5．4％，土壤含水量提高9．8％，减轻土壤容重３％,林分虫害明显下降，油松树高、胸径生长量分别提高22．6％、19．8％。     

山地橄榄主要病虫害种类及其防治措施

介绍了山地橄榄2种病害：叶斑病、炭疽病；5种虫害：橄榄星室木虱、黑刺粉虱、恶性叶甲、橄榄枯叶蛾、橄榄野螟的主要特征和危害症状，并提出了防治措施。     

油研9号氮、钾、硼肥的施用技术

为了探讨油菜良种良法配套栽培技术,以新品种油研9号进行了氮肥的不同施用比例、钾肥的不同施用量、硼肥的不同施用方式的研究。结果表明:在相同施氮量下,以基肥:苗肥:薹肥为3:4:3时产量最高,与5:3:2和6:2:2 的施用比例达显著性差异;与4:3:3的施用比例差异不显著。不同钾肥施用量以底施氯化钾15 kg／667m2产量最高,底施氯化钾均与不施氯化钾达极显著性差异,但底施氯化钾15 kg／667m2比底施氯化钾7．5 kg／667m2增产不显著。不同硼肥的施用方式,以薹施硼肥1 kg／667m2产量最高,薹施(1kg／667m2)、底施(1 kg／667m2)、花喷(100g／667m2)硼肥分别比不施硼肥增产 31．5％、22．8％、5．5％,施用硼肥与不施硼肥均达显著性差异,薹施和底施硼肥增产效果明显,花喷增产效果较差。     

Markers, old and new, for examining Phytophthora infestans diversity

Late blight, caused by Phytophthora infestans , is an ongoing threat to potato and tomato crop production worldwide and considerable fundamental and applied research is conducted with the long-term aim of improved disease control. Understanding the mechanisms, processes and rates of P. infestans evolution is an important factor in predicting the effectiveness and durability of new management practices. A range of phenotypic and genotypic tests has been applied to achieve this goal, but each has limitations and new methods are sought. Recent progress in P. infestans genomics is providing the raw data for such methods and new high-throughput codominant biomolecular markers are currently being developed that have tremendous potential in the study of P. infestans population biology, epidemiology, ecology, genetics and evolution. This paper reviews some key applications, recommends some changes in approach and reports on the status and potential of new and existing methods for probing P. infestans genetic diversity.     

钴—60辐照对石花菜琼胶质量影响

用钴—60辐照处理琼胶成品和干制石花菜。经钴—60辐照的琼胶成品,其凝胶强度均有所下降,其下降率与辐照剂量大小呈正比。石花菜经钴—60辐照后,可使琼胶提取率提高,但过高的辐照剂量,也同时会降低所制取琼胶的凝胶强度。通过适宜剂量的钴60—辐照处理干制石花菜,可提高琼胶提取率3％～5％,且制取的琼胶凝胶强度不受辐照的影响。     

奶牛冷冻胚胎规模化移植效果研究

为了提高奶牛良种化程度,对奶牛冷冻胚胎的规模化移植效果进行了研究。结果表明:PG二次肌注法的发情率显著高于(P<0.05)PG一次肌注法,两种方法的发情同期率均超过85%,受体牛发情时间多集中在肌注PG后48～72h;胚胎解冻后的复苏率达到82.58%,且早期囊胚与囊胚的复苏率要高于桑椹胚(P>0.05);共移植受体牛218头,受胎97头,受胎率为44.5%,且春、秋季的移植效果差异不显著(P>0.05)。为奶牛冻胚的规模化移植研究提供了参考依据。     

In vitro Plant Regeneration from the Mature Tissue of Navel Orange (Citrus sinensis L. Osbeck) by Direct Organogenesis

An efficient in vitro regeneration system by direct organogenesis from mature nodal and internodal stem segments of Newhall navel orange (Citrus sinensis L. Osbeck) was developed. Illuminating conditions together with plant growth regulators affected the adventitious bud regeneration frequency and efficiency. The initial 15 d darkness inoculation is beneficial for the adventitious bud regeneration. The highest regeneration frequency (85.2%) and bud formation efficiency (3.7 per responsive internodal stem segment) were obtained in the media supplemented with 1.0 mg L-1 BAP and 0.5 mg L-1 NAA. ABA at 0.2 mg L-1 positively affected the bud formation efficiency, which amounted to 8.5 buds per internodal segment in the presence of BAP at 1.0 mg L-1. The adventitious shoots successfully rooted and were transferred to the soil.