Expression of cathepsin C and tumor necrosis factor-α in human coronary artery and their relationships with coronary heart disease

AIM To explore the possible mechanism of cathepsin C （CTSC） and tumor necrosis factor-α （TNF-α） in coronary heart disease （CHD） by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees （P<0.05）. The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group （P<0.05）. Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group （P<0.05）. The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD （r²=0.743, P<0.05）. CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD.     

吴茱萸嫁接方法试验

试验表明:5种嫁接方法苗木成活率均在80％以上,其中以贴枝接和长块削芽接为最高,平均成活率达95％以上,但从快速繁育苗木着眼,以长块削芽接为好。嫁接适期以3月中旬树液流动时为最好。嫁接苗当年在圃地就能开花结果。     

Legume–barley intercropping stimulates soil N supply and crop yield in the succeeding durum wheat in a rotation under rainfed conditions

Legume–cereal intercropping is increasingly being appreciated in dryland areas, where severe climatic conditions and intensive agricultural practices, generally dominated by continuous cereal cultivation, determine depletion of soil nutrient resources and decline of soil fertility. This research aimed to assess whether and to what extent a newly introduced legume-based intercropping system is able to ameliorate the biological fertility status of an arable soil in a way that is still noticeable during the succeeding durum wheat cropping season in terms of changes in bacterial community structure, soil C and N pools, and crop yield. A field experiment was carried out under rainfed conditions in Southern Italy on a sandy clay loam soil cultivated with durum wheat following in the rotation a recently established grain legume (pea, faba bean)–barley intercropping. Soil chemical, biochemical and eco-physiological variables together with compositional shifts in the bacterial community structure by LH-PCR fingerprinting were determined at four sampling times during the durum wheat cropping season. Soil fertility was estimated by using a revised version of the biological fertility index. Results showed that even though the microbial biomass was significantly altered, the preceding legume intercrops stimulated C-related functional variables thus leading to an increased release of mineral N, which was larger in crop treatments succeeding pea-based than faba bean-based intercropping. The increased N made available in soil enabled the succeeding durum wheat to achieve an adequate grain yield with a reduced N-fertilizer use. Soil type and environmental conditions rather than crop treatments were major determinants of bacterial community structure. The biological fertility status was not varied, suggesting that in intensively managed rainfed areas long-term crop rotations with intercropped legumes are needed to consistently ameliorate it.     

Erträge,n‐entzüge und humusdynamik im statischen düngungsversuch bad lauchstädt, 18 jahre nach erweiterung der versuchsfrage∗

Im Statischen Düngungsversuch Bad Lauchstädt werden nach Erweiterung der Versuchsfrage seit 1978 die Prüffaktoren: Stalldung (2 Stufen), C_org‐Gehalt im Boden (6 Stufen) und Mineral‐N‐Mengen (5 Stufen) in der Fruchtfolge Kartoffeln, Winterweizen, Zuckerrüben, Sommergerste geprüft. Nur bei Sommergerste konnte eine Ertragswirkung des Stalldungs im Vergleich zu ausschließlicher Mineraldüngung nachgewiesen werden. Die jährlichen Veränderungen der C_org‐ und N_t‐Gehalte im Boden betrugen bei jeweils extremen Änderungen des Düngungsregimes bis zu 0, 012% C_org bzw. 0,0013% N_t.     

8%胺鲜酯水剂在白菜上应用药效试验

用8%胺鲜酯水剂在大白菜上进行田间药效试验,试验表明,在大白菜的大田返青期、生长期喷施,能有效地促进大白菜的生长,提高大白菜的产量。对大白菜中的维生素C和可溶性固形物含量等品质指标未发现有不良影响,且对品质有所提升。     

Significance of salt transport patterns in rice varieties differing in salt tolerance

Abstract

The absorption mechanisms for Na, K, SO₄ and Cl were tested in a salt tolerant (PVR 1) and a salt sensitive (GEB 24) rice varieties. The salt tolerant variety accumulated significantly larger amounts of Na than the salt sensitive variety. Further, PVR 1 absorbed SO₄ from Na₂SO₄ in preference to that from K₂SO₄. The absorption patterns for K and Cl were similar in both the varieties. It is concluded that the capacity of plant species to accumulate greater amounts of Na is a reflection of their halophytic feature.     

犬流感研究进展

甲型H1N1流感病毒的暴发使得流感病毒的相关研究受到了前所未有的关注,尽管流感病毒的易感种群正在不断扩大已成为事实,但是长久以来的观念仍然认为犬不属于流感病毒的易感动物,直到2004年犬流感病毒H3N8亚型被证实后这种认识才得以改变。论文综述了犬流感病毒H3N8这个犬类新型传染病的病原学和流行病学研究概况,对于了解、研究和预防该病具有借鉴意义。     

大豆高赖氨酸基因PM8在烟草中的超量表达及其抗非生物胁迫功能研究

应用生物信息学方法在GenBank数据库查找到1条编码大豆LEA3蛋白的高赖氨酸蛋白基因PM8(登录号:Z22872),并利用RT-PCR方法从大豆干种子中克隆得到该基因,序列分析表明,PM8基因与GenBank上的序列存在5个碱基的差异,使编码蛋白的赖氨酸重量比由原来的19.65%增加为19.93%。构建了PM8基因的植物表达载体pEMT-PM8,用农杆菌介导法转化烟草。从转PM8基因的PCR阳性植株中选取5个既抗盐又抗旱的株系进行Real-timePCR检测。结果表明,PM8基因全部超量表达。研究为培育高营养品质且抗逆的转基因作物研究提供了一条新的途径,具有重要的研究价值。     

焊接温度检测系统的研究

分析了焊接温度对焊接质量的重要影响,应用传感器技术、温度数据采集技术和计算机通信技术,设计了一种基于AT89C52单片机的温度检测系统。实验结果显示,该系统能够很好地对焊接温度进行采集与处理,为实现研究焊接过程温度场的测量打下了良好的基础。     

英语双及物结构的构式语法观

构式语法是以认知语言学为理论背景的语言研究方法。本文以Goldberg所倡导的构式语法理论为指导，对双及物结构予以剖析，旨在探究双及物结构这一特定构式的句法和语义特征，同时构建出双及物结构的构式表征模式，以揭示该构式的本质。