New aspects of soybean somatic embryogenesis

Somatic embryo formation from immature cotyledons was improved in the following ways: by cutting into sections, supplementing culture media with spermine and using solid/liquid/solid type of culture. Cut cotyledons of the eight genotypes examined expressed a higher ability for somatic embryogenesis than whole cotyledons. Of the three polyamines tested, spermine considerably stimulated and putrescine slightly inhibited induction of somatic embryos. The ability of embryoid formation on medium with spermidine depended on the genotype. The solid/liquid/solid type of culture was better than the continuous solid culture. The best nitrogen ion content for the subculture of somatic embryos was 10 mM NH₄NO₃ and 30 mM KNO₃. The possibility of using these modifications in Agrobacterium transformation is discussed.     

HRT Effects on Biological Nutrient Removal in Spiral Up-flow Reactor System

Overall effects of HRT ( 11 h, 9 h, 8 h, 6 h ) on the Spiral up-flow reactor (SUFR) system are analyzed. The results of the operation under different HRT condition shows that the removal efficiencies of COD, TN and TP decreased not obviously with decreasing HRT when HRT is longer than 8h. Anaerobic retention time, anoxic retention time and aerobic retention time were 1.51 h,2.25 h and 4.24 h,respectively. The removal efficiencies of COD, TN and TP is kept over 93%,87% and 90.2%, respectively. Although the removal efficiencies changed obviously when the HRT was 6 h. Anaerobic retention time, anoxic retention time and aerobic retention time are 1.13 h,1.69 h and 3.18 h,respectively. COD-removal efficiency is always up to 85%, the removal efficiencies of TN and TP is kept over 76% and 72%,also has upper removal efficiencies. It is indicated that the SUFR system, which is similar to plug-flow-reactor, has strong stability and can compensate negative effects of increasing hydraulic loading.     

Effect of varied N rates and N timings on yield, N uptake and fertilizer N use efficiency of a six-row and a two-row winter barley

The effects of N rates and N timings on yield formation, N uptake at five growth stages and fertilizer N use efficiency of six-row and two-row winter barley were evaluated in field trials conducted from 1990/91 to 1992/93 at the TU Munich's research station Roggenstein.

On average over 3 years the six-row cultivar yielded most at a total rate of 110 kg ha⁻¹ N including an early application of 40 kg ha⁻¹ N up to EC 30 (Zadoks scale). The two-row cultivar achieved maximum yield at a total rate of 140 kg ha⁻¹ N including early applications of 70 kg ha⁻¹ N up to EC 30. The highest yielding N-treatments of six-row barley regularly took up less nitrogen at EC 32 (95 kg ha⁻¹ N on average) than the non-optimally fertilized treatments, whereas full exploitation of the yield potential of two-row barley was associated with higher rates of N-uptake at EC 32 (113 kg ha⁻¹ N on average).

Lodging did not occur in the trials conducted in 1991 and 1992 and no difference was detected between the two cultivars in fertilizer N use efficiency. With six-row barley the N treatment giving maximum yield also led to an optimum fertilizer N use efficiency. Full exploitation of the two-row barley yield potential was associated with suboptimal fertilizer N use efficiencies.     

不同处理对蘑菇与黄豆芽混合汁营养成分的影响

采用不同工艺条件测定蘑菇与黄豆芽混合汁中营养成分的含量。经测定得知,蘑菇：黄豆芽：水：1：2：3,利用内源酶水解24h,混合汁中水溶性含氮物、VC含量较高,利用内源酶或盐酸水解可显著提高还原糖浓度。     

作物水分利用率的影响因素及其提高途径探讨

综述了作物水分利用率的研究进展。在探讨水分利用率内涵的基础上，阐述了作物水分利用率的影响因素如作物，环境因素，化学制剂，栽培措施和耕作制度等人类活动等影响因素，重点分析了土壤因素和化学制剂对作物水分利用率的影响，探讨了作物水分利用率的提高途径，包括优化种植结构；选用节水高产型品种；农田覆盖保水技术；水肥耦合技术；化学调控节水技术和节水灌溉技术等     

Tillage, cover crops, and nitrogen fertilization effects on soil nitrogen and cotton and sorghum yields

Sustainable soil and crop management practices that reduce soil erosion and nitrogen (N) leaching, conserve soil organic matter, and optimize cotton and sorghum yields still remain a challenge. We examined the influence of three tillage practices (no-till, strip till and chisel till), four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secaele cereale L.)], vetch/rye biculture and winter weeds or no cover crop}, and three N fertilization rates (0, 60–65 and 120–130 kg N ha⁻¹) on soil inorganic N content at the 0–30 cm depth and yields and N uptake of cotton (Gossypium hirsutum L.) and sorghum [Sorghum bicolor (L.) Moench]. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic, Plinthic Paleudults) from 1999 to 2002 in Georgia, USA. Nitrogen supplied by cover crops was greater with vetch and vetch/rye biculture than with rye and weeds. Soil inorganic N at the 0–10 and 10–30 cm depths increased with increasing N rate and were greater with vetch than with rye and weeds in April 2000 and 2002. Inorganic N at 0–10 cm was also greater with vetch than with rye in no-till, greater with vetch/rye than with rye and weeds in strip till, and greater with vetch than with rye and weeds in chisel till. In 2000, cotton lint yield and N uptake were greater in no-till with rye or 60 kg N ha⁻¹ than in other treatments, but biomass (stems + leaves) yield and N uptake were greater with vetch and vetch/rye than with rye or weeds, and greater with 60 and 120 than with 0 kg N ha⁻¹. In 2001, sorghum grain yield, biomass yield, and N uptake were greater in strip till and chisel till than in no-till, and greater in vetch and vetch/rye with or without N than in rye and weeds with 0 or 65 kg N ha⁻¹. In 2002, cotton lint yield and N uptake were greater in chisel till, rye and weeds with 0 or 60 kg N ha⁻¹ than in other treatments, but biomass N uptake was greater in vetch/rye with 60 kg N ha⁻¹ than in rye and weeds with 0 or 60 kg N ha⁻¹. Increased N supplied by hairy vetch or 120–130 kg N ha⁻¹ increased soil N availability, sorghum grain yield, cotton and sorghum biomass yields, and N uptake but decreased cotton lint yield and lint N uptake compared with rye, weeds or 0 kg N ha⁻¹. Cotton and sorghum yields and N uptake can be optimized and potentials for soil erosion and N leaching can be reduced by using conservation tillage, such as no-till or strip till, with vetch/rye biculture cover crop and 60–65 kg N ha⁻¹. The results can be applied in regions where cover crops can be grown in the winter to reduce soil erosion and N leaching and where tillage intensity and N fertilization rates can be minimized to reduce the costs of energy requirement for tillage and N fertilization while optimizing crop production.     

密度和追肥时期对重穗型冬小麦品种L906群体辐射和光合特性的调控效应

密度和追肥时期对重穗型高产冬小麦的群体发展,冠层辐射和光合特性有明显的调节效应.随着密度增加,开花前的叶面积指数(LAI)、群体净光合速率(NCP)、群体叶源量(CLSC)提高,但生育后期衰减较快;密度过高导致群体透光率下降,消光系数增加,单茎受光量降低,冠层光环境恶化,后期旗叶的羧化效率降低;密度过低则导致生育前期漏光损失     

肥料深施对小麦生育性状、养分吸收及产量的影响

几年来在豫东潮土地区砂质土上进行的肥料深施效果试验研究结果表明,肥料深施对小麦的生育状况和经济性状有较大影响,肥料深施与浅施比较,可使小麦植株前期生长健壮,中后期生长稳健,落黄好,穗大粒多,籽粒饱满。可显著增加小麦养分吸收量,提高化肥利用率,肥料深施处理比浅施处理氮、磷、钾的吸收量,分别增加34.32￣67.81kg/hm2,3.22￣9.42kg/hm2和17.82￣39.84kg/hm2;氮素化肥利用率提高20.86￣41.1个百分点,磷肥利用率提高7.13￣15.39个百分点;肥料深施在小麦田里养分残留量较浅施的高,尽管一季小麦对土壤有机质耗竭不大,但对氮、磷素的残存已有差异。肥料深施对小麦产量、经济效益、化肥利用率有显著的影响,采用肥料深施技术比群众习惯施肥方法(浅施)可增产小麦337.1￣1183.5kg/hm2,增产率达7.6%￣26.6%,增加收益539.36￣1893.6元/hm2。     

不同施氮水平下棉花生物量动态增长特征研究

在大田实验条件下,以定量方法系统地分析了黄淮海棉区(以河南安阳为试点)不同施氮水平(每公顷0 kg、120 kg、240 kg3、60 kg、480 kg)下棉株生物量的动态累积特征,结果表明:棉株地上、地下部分干物重的动态累积符合Logistic生长曲线,生物量的增长对氮肥施用量较为敏感;施肥量为360 kg.hm-2时棉田具有快速增长期启动早、持续时间短、增长速率大等特征,说明该施氮量利于棉花生长前期的生物量的快速累积,氮肥施用量过多或过少均不利于合理棉花群体的建立;不同的施氮量可以调节快速生长期的早晚和持续时间,同时亦可改变其干物质累积速率。生产中可以通过调节氮肥的施用量与时间获得高产。对于黄淮海棉区,适宜的施肥量为360 kg.hm-2,7月中旬为棉田肥水管理的关键期。     

关于上一轮县级土地利用总体规划中土地利用变化及生态环境效应的思考 ——以重庆市丰都县为例

基于重庆市丰都县1996年、2004年土地利用现状与2010年土地利用总体规划数据，运用特尔斐法、层次分析法、定量分析法，对丰都县1996—2004和1996—2010土地利用变化及生态环境效应进行对比定量分析研究。结果表明：（1）土地利用格局均发生了明显变化，耕地、未利用土地减少，园地、林地、水域、交通用地增加；（2）土地利用变化引起的生态环境质量变化均呈改善趋势，其主要原因是退耕还园、还林，以及未利用土地的开发利用；（3）规划与实施土地利用变化及生态环境效应差异太大；（4）土地利用总体规划与实际土地利用变化一致性很差，缺乏科学性和严肃性；（5）对规划土地利用变化引起的生态环境改善重视不够。最后提出新一轮土地利用总体规划修编的建议。