Transplantation of adipose derived mesenchymal stem cells for acute thoracolumbar disc disease with no deep pain perception in dogs

Thirty-four dogs with no deep pain perception due to acute thoracolumbar intervertebral disc disease underwent decompression surgery within 1 week of diagnosis. All dogs underwent hemilaminectomy. Adipose derived mesenchymal stem cells (AD-MSCs) were transplanted into the injured spinal cord parenchyma for the AD-MSCs transplant dogs. Long-term outcome was evaluated at the end of the follow-up period (> 6 months). AD-MSCs combination treatment showed better recovery outcomes compared to decompression surgery alone. These results indicate that this stem cell therapy is a potential therapeutic strategy to overcome the limitations of treatment for spinal cord injury in clinical medicine.     

牛磺胆酸对小鼠体内氧自由基的影响

为了研究牛磺胆酸(TCA)对小鼠体内氧自由基代谢的影响,采用黄嘌呤氧化酶法、二硫代二硝基苯甲酸法、紫外分光光度法、硫代巴比妥酸法和酶联免疫特异性测定法分别测定了给药后小鼠血浆、肝脏和脑组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)的活力、脂质过氧化产物丙二醛(MDA)的含量及血浆中一氧化氯合酶(NOS)的活力.结果显示,低剂量TCA能显著提高血浆、肝脏和脑组织中GSP-PX活力及肝脏和脑组织中CAT活力,显著降低血浆中GSH-PX活力和脑组织中GSH-PX活力.高剂量的TCA能显著提高血浆中GSH-PX、肝脏和脑组织中T-SOD、GSH-PX及肝脏中CAT活力.低、高剂量的TCA能显著降低血浆、肝脏和脑组织中MDA的含量,以及提高血浆中NOS活力.结果证明,TCA的抗氧自由基作用与其提高体内SOD、GSH-PX、CAT、NOS的活力和降低MDA含量有关.     

Protective effect of BNDF on vascular endothelial cells with H2O2-induced oxidative injury

AIM: To study the protective effect of brain-derived neurotrophic factor (BDNF) on vascular endothelial cells with H₂O₂-induced oxidative injury. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro, and the oxidation injury model of HUVECs was established by treatment with H₂O₂. The oxidatively injured HUVECs were cultured with different concentrations (1, 10 and 100 μg/L) of BDNF. At the same time, the control group (no injury), PBS treatment after H₂O₂ injury group and TrkB inhibitor group (with 100 μg/L BDNF and 1: 1 000 TrkB inhibitor) were also set up. The viability of the HUVECs was detected by MTT assay. The levels of LDH, MDA, SOD and GSH were measured. The releases of NO, ET-1 and ICAM-1 were analyzed by ELISA. The changes of ROS production and cell apoptosis were evaluated by flow cytometry. The protein levels of TrkB, p-TrkB, cleaved caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with uninjured control group, in H₂O₂ oxidative injury plus PBS treatment group, the viability of the cells was decreased significantly, the LDH and MDA levels were increased significantly and the activities of SOD and GSH were decreased significantly. The NO secretion was decreased, and the ET-1 and ICAM-1 concentrations were increased significantly. The ROS content and apoptotic rate were increased significantly. The protein levels of cleaved caspase-3 and Bax were increased but Bcl-2 protein expression was decreased significantly. Compared with PBS treatment group, in H₂O₂-injured HUVECs treated with different concentrations of BDNF, the cell viability was gradually increased, the LDH and MDA levels were decreased and the activities of SOD and GSH were increased gradually. The secretion of NO was increased but ET-1 and ICAM-1 were decreased gradually. The ROS content and apoptotic rate were decreased significantly. The TrkB and p-TrkB levels were significantly increased significantly, the protein expression of cleaved-caspase 3 and Bax was decreased gradually and the Bcl-2 protein expression increased gradually. The role of BDNF was inhibited by TrkB inhibitor. CONCLUSION: BDNF protects HUVECs from oxidative injury by binding with TrkB to activate the BDNF-TrkB signaling pathways.     

动物源性抗氧化成分及应用研究进展

本文对动物源性抗氧化物的种类（抗氧化肽、糖及其衍生物、酶类等）、分离提取及应用现状进行了综述。     

黄泛沙区冬小麦施钾效应研究

研究了施钾对冬小麦的生长及产量的影响。结果表明，黄泛沙区中低产田冬小麦钾素与N、P、Zn配合施用的最佳经济施钾量为225～300kg／hm^2，比对照增产17．6％～23．2％，经方差分析达显著水平。钾素能增加穗粒数，提高粒重，提高茎秆充实度，增强抗倒伏能力。     

污泥衍生吸附剂对废水中酸性雪青的吸附特性研究

[目的]研究污泥衍生吸附剂对废水中酸性雪青的吸附特性.[方法]以污泥粉为原料,加入锯末,用氯化锌活化,高温热解制得一种污泥衍生吸附剂(碘值为462.36 mg/g),并研究了污泥衍生吸附剂对废水中酸性雪青的吸附特性.[结果]污泥衍生吸附剂对酸性雪青吸附动力学模型拟合结果表明,100、300、400 mg/L的酸性雪青溶液都符合假二级动力学模型,其R2都＞0.99; 100、300、400 mg/L的酸性雪青溶液到达平衡的时间分别是30、90、150 min,低浓度到达平衡的时间短,高浓度到达平衡时间长.根据热力学试验可以得出,其吸附过程在10、20、30℃下都可用Langmuir和Freundlich吸附模型很好地描述(其R2都＞0.99).根据其吸附过程与液膜扩散模型和颗粒内扩散模型的拟合情况,得到液膜扩散是其吸附过程的速率控制步骤,但不是唯一速率控制步骤,而颗粒内扩散并不是其过程的速率控制步骤.[结论]使用污泥衍生吸附剂吸附废水中酸性雪青具有可行性.     

甘蓝型油菜单倍体植株群体的构建

以油菜双低（低芥酸、低硫苷）品种＂沪油12＂的小孢子来源植株为试验材料,研究了供体植株不同外植体部位不定芽再生情况及影响不定芽诱导的一些因素,并对甘蓝型油菜小孢子来源的再生组培苗进行了染色体倍性的鉴定.结果表明,利用节间茎段进行不定芽诱导是一种较好的快速再生植株方式,具有出芽快,遗传特性保持好的特点.茎段具有较强的不定芽分化能力,而叶柄和叶片难以诱导分化出芽.通过优化培养基,建立了甘蓝型油菜小孢子来源植株茎段高频不定芽再生系统.通过对甘蓝型油菜组培苗的叶片气孔保卫细胞和根尖染色体数目的观察,确定了一批小孢子来源的单倍体植株.     

红壤稻田养地体系的研究Ⅰ．有机、无机肥结合对红壤稻田生产效率的影响及其养地效果

多点定位田间试验表明．在福建中低产红壤稻田中混合施用有机和无机肥料，可使水稻生产效率在短期内得到稳定增长（３０％－９２％）．经统计分析比较，水稻生产效率与有机无机总养分（或总氮）投入量呈极显著相关；有机无机肥结合的优点主要表现在土壤生产力的稳定积累和元素生产效率的提高．参试的４大有机给源配合半量化肥的作用近于等效，但在试验期限内（１－３ａ），稻谷产量无额外的互作效应，而绿肥鲜体在增产养地配合效应上具有明显的优势．     

基因型与环境互作对黄早四及其衍生系农艺性状的影响

选用黄早四及其衍生系共30份自交系为材料,通过在不同生态条件下两年三点的大田试验,对农艺性状的稳定性进行研究。结果表明,农艺性状在哈尔滨表现最好,有利于产量水平的发挥;农艺性状中受GEI影响程度最大的是百粒重,其次为单株产量。文章对黄早四及其衍生系产量的稳定性比较得出高产稳产型自交系,同时对黄早四及其衍生系的改良方向进行了探讨。     

液相色谱—串联质谱法测定动物源性食品中4种β_2~-受体激动剂类药物残留

建立了动物源性食品中4种β2-受体激动剂克伦特罗、莱克多巴胺、沙丁胺醇和特布他林的高效液相色谱—串联质谱测定方法。样品经5%的三氯乙酸酸解提取,Waters Oasis MCX固相萃取柱净化,然后用Aglient ZORBAXSB-Aq(3.5μm,3.0 mm×100 mm)色谱柱分离,以0.1%甲酸乙腈溶液和0.1%甲酸水溶液为流动相进行梯度洗脱,基质匹配标准溶液内标法定量。结果表明,4种β2-受体激动剂在质量浓度1.0~100 g/L内呈良好的线性关系,相关系数R均大于0.995 0;检出限(S/N≥3)均为0.1μg/kg,定量下限(S/N≥10)为0.25 g/kg,在0.5,2,5μg/kg3个质量分数添加水平,回收率在81%~108%,相对标准偏差在1.3%~9.4%。方法灵敏度高、定性准确可用于各种动物源性食品中4种β2-受体激动剂残留的快速检测。