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61.
ZHANG Li-rong XIANG Peng XIA Wen-jie CHEN Zhen-guang ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2002,18(8):896-899
AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK. 相似文献
62.
AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D3 line(ES-D3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC. 相似文献
63.
Traditional concept has been that hematopoietic stem cells (HSC) are tissue-specific stem cells, which are restricted to generate the cell types of the blood and immune system. However, recent studies have shown that there is a higher plasticity of the HSC than previous expected, it can be induced to transdifferentiated into mature cells of other nonhematopoietic organs after transplantation in vivo. In this article, the recent advances in the plasticity of HSC and its potential clinical application are reviewed. 相似文献
64.
稻瘿蚊间歇性暴发原因和治理对策探讨 总被引:3,自引:0,他引:3
通过多年的系统调查和观察,明确了稻瘿蚊发生具有间歇性、隐蔽性、暴发性、毁灭性和专一性等5个特点,分析了其在大田县间歇大发生有5个方面的因素。防治上提出农业防治可采取“铲”、“避”、“改”等方法,化学防治应选用具有强触杀、强渗透力药剂,采用毒土法施药技术。 相似文献
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引进扁桃的品质特性观察 总被引:6,自引:0,他引:6
从美国引进的3个扁桃品种,用山桃作砧木,在陕西渭北地区生长旺盛,苗木栽植第3年开始开花结果,5年生平均株产1.78~2.03kg。扁桃3月中下旬开花,果实前期发育较快,至5月中旬果径生长量占总量的80%~90%,后期增长缓慢。Nonpareil品种7月25日~8月5日成熟,Mission品种9月11日~9月15日成熟。成熟种仁饱满,外观品质较好。种仁含粗脂肪49.62%~52.49%,含粗蛋白20.9%~29.0%,含VB11.8~1.91mg/kg,VB24.08~4.62mg/kg。氨基酸及钙、镁等元素含量也较丰富,与我国新疆扁桃主产区产品营养含量相近似。 相似文献
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1999-2002年在内蒙古锡林郭勒盟白音锡勒牧场典型草原区海流特,监测主要植物群落结构特征。结果表明,随时间推移,试验区主要群落的优势植物大针茅、羊草相对高度、相对盖度、相对密度和相对生物量呈降低趋势,退化指示植物冷蒿、狼毒相对高度、相对盖度、相对密度、相对生物量呈增加趋势;试验区群落物种丰富度指数、多样性指数和均匀性指数随时间推移均呈降低趋势;围栏在试验区草地植物群落特征的保持方面很有效,但围栏内植物群落特征随时间推移仍呈退化趋势。 相似文献