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471.
Sheila Mae S Santander‐de Leon Sharon N Nuñal Hongyi Wei Takeshi Yoshikawa Hiroto Maeda 《Aquaculture Research》2017,48(8):4380-4389
This study investigated the influence of fish feed deposition on the microbial community of sediments in a coastal lake. Microcosms were designed to simulate the addition of fish feeds to the sediment, and physicochemical factors as well as composition of microbial assemblages were analysed. Although total organic carbon and total organic nitrogen did not significantly increase, redox potential and total sulphides showed a transition of sediment environments into anaerobic condition due to the addition of fish feeds. The bacterial community composition shifted with fish feed deposition as revealed by the band profiles of denaturing gradient gel electrophoresis, Sørensen's similarity indices and cell numbers of sulphate‐reducing bacteria. Occurrence of anaerobic bacteria (sulphate and nitrate reducers) and disappearance of strictly aerobic bacteria were observed with fish feed inputs. The results clarify the succession of the microbial flora and suggest negative impact of uneaten fish feeds on the microbial community in sediment and resultant environmental deterioration of fish‐farming areas. 相似文献
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This study was intended to evaluate tannin mediated inhibition of digestive proteases in two different size groups (fingerlings, F, 4.5 ± 0.7 cm; advanced fingerlings, AF, 18.2 ± 1.6 cm) of rohu, Labeo rohita. Graded levels (50, 100, 150 and 200 nM) of tannin (Gallotannin, 99% purity) were added to the enzyme extracts (30°C, 1 hr) prior to determination of enzyme activities. Changes in the activity of trypsin, chymotrypsin and total protease in relation to the control sets were determined through biochemical assay of enzymes and SDS‐PAGE zymography. The study revealed that tannin significantly inhibited trypsin, chymotrypsin and total protease activities in a dose dependent manner as evident from the regression equations. The degree of the inhibition appeared to be significantly higher for the F in contrast to the AF (F5,66 = 282.311; p < 0.0001). Trypsin activities were reduced by 8.04 ± 0.19% to 52.68 ± 0.72% and 5.61 ± 0.22% to 39.46 ± 0.19% in F and AF, respectively. The reduction in Chymotrypsin activities ranged between 16.11 ± 0.03% to 38.02 ± 0.27% in F and 6.31 ± 0.07% to 22.80 ± 0.32% in AF. Total protease activities were reduced by 10.9 ± 0.07% to 49.60 ± 0.32% in L. rohitaF, whereas, it ranged between 5.19 ± 0.06% to 32.60 ± 0.13% in AF. On a comparative scale, the difference in tannin induced inhibition in F and AF were more prominent for trypsin and chymotrypsin than total protease. Further, nine protease activity bands (15.9–69 kD) with different electromobility were noticed in both the size groups. Subsequent densitometry analysis revealed that the average densities of the protease activity bands were gradually decreased with increasing level of tannin exposure (50–200 nM). The study might indicate adaptive tolerance to tannin in larger size groups and emphasizes the need for removal of tannin in the plant feedstuffs, especially for feeding the fingerlings of L. rohita. 相似文献
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为了探讨混凝法去除水中纳米颗粒的可行性及最佳条件,研究了无机混凝剂(PAC、PFS、PAFC)和有机絮凝剂(CPAM、APAM、NPAM)对TiO2纳米颗粒的去除效果,并考察了投加量、pH、沉淀时间、水力条件及有机无机复配对TiO2纳米颗粒去除效率的影响。单独投加PAC、PFS和PAFC时,三者对应的最高去除率分别为92.51%、84.43%、95.66%。单独投加CPAM、APAM、NPAM时三者对应的去除率仅为61.72%、29.06%、55.37%。复配最佳混凝条件为:投加40 mg/LPAC和3 mg/LCPAM,pH值为9,G值143.5/s,沉淀时间15 min,此时,TiO2纳米颗粒去除率为99.6%。 相似文献
476.
土体的沉积作用和应力历史会影响土体自重应力的确定,也会影响地基最终沉降量的确定。鉴于e-lnp比e-lgp坐标图能更方便、精确地描述线性问题,根据e-lnp坐标图推导沉积作用对成层土、正常固结土、超固结土的自重应力与沉降影响的计算式。用该计算式对昆明滇池国际会展中心部分深厚软土地基的自重应力和最终沉降量进行计算,并用计算结果和不考虑沉积作用及应力历史影响的计算结果进行对比分析,结果表明,沉积作用对深厚软粘土自重应力和最终沉降量计算值的影响非常显著,并且土体厚度越厚、上覆荷载越大,其影响越明显。 相似文献