促性腺激素释放激素基因及其受体基因的研究进展

作者简要介绍了促性腺激素释放激素基因及其受体基因的位置、结构、表达、调控机理,并初步探讨了这两个基因与繁殖性能的关系。     

利用雄性不育选育辣椒新品种农大082

农大082是以雄性不育系S200243A为母本，恢复系S200244C为父本配制而成的微辣型辣椒一代杂种。中早熟，从定植到始收30d（天）左右，果实羊角形，果纵径17—20cm，横径3-4cm，嫩果深绿色，成熟果红色，味微辣，果肉脆嫩，平均单果质量40—60g，667m^2产量3200-4600kg,抗TMV，耐CMV。已在北京、河北、四川、黑龙江等地示范推广逾400hm^2。     

哺乳动物附植前胚胎的基因表达调控

哺乳动物胚胎附植前期包括:合子的形成,胚胎基因组的激活和细胞分化的开始。在这个时期,发育由母源物质控制转为合子基因控制,在此过程,同时形成染色质介导的转录抑制时期,要解除抑制必须经过胚胎基因组的激活。通过对体内、外附植前胚胎的mRNA的表达特点以及它们与成功发育联系的研究,可以筛选出最佳的体外培养条件,设计最佳的核移植方案。     

桑树新品种育71-1的育成初报

育71—1是通过杂交、单株选择、株系选择和品比试验等一系列选育程序,培育成功的新桑树品种。其主要经济特性与湖桑32号相比,发芽期早2—3天,发芽率提高28.7％,产叶量提高47.7％,叶质较优,丝质成绩相仿;抗旱性较强。目前,正在江苏的海安、溧阳及山东等部分地区试种。     

Glycosylated hemoglobin in dogs: precision, stability, and diagnostic utility

The precision and stability of the ion exchange chromatography assay for canine glycosylated hemoglobin (HbA(1)) were examined. The coefficient of variation (CV) of within-run replicate assays was 1.3 to 2.6%; the CV of between-run duplicate assays was 3.1%. The mean HbA(1) content in 44 healthy dogs was 7.1% (SD = 1.1%, range = 5.1-9.7%). Paired aliquots of 12 blood samples were stored at 4 degrees C and 25 degrees C, and HbA(1) was measured on the day of collection and at 3, 5, and 7 days after collection. In the blood stored at 4 degrees C, no significant increase in the HbA(1) content was seen. No significant increase in HbA(1) content was found in the blood stored at 25 degrees C after 3 days, but dramatic increases were observed after 5 and 7 days of storage. No significant difference was observed in the HbA1 content in heart blood collected 18 hours after death from 9 dogs kept at 25 degrees C. The HbA(1) content was measured in 10 hospitalized diabetic dogs. Five of the dogs had received no insulin and all 5 had elevated HbA(1) values. The other 5 dogs had received insulin for 1 to 9 months; 2 of the 5 had increased HbA(1) content. The HbA(1) content was determined periodically for 9 months in one diabetic dog and it declined from 14% to 8.2%.     

苜蓿银纹夜蛾核多角体病毒在家蚕蛹体内的复制及重组病毒外源基因的表达

家蚕蛹在复眼着色期,经4℃冷藏24小时后,可被Ac NPV(苜蓿银纹夜蛾核多角体病毒)感染。在蛹体内复制出的多角体大小差异较大,且比在昆虫Sf—21细胞中繁殖的Ac NPV和在蚕蛹体内形成的Bm NPV多角体小。在蛹体内繁殖的Ac NPV的游离病毒可以回返感染Sf—21细胞。由蚕蛹内分离的Ac NPV基因组DNA的限制性内切酶图谱与野生型的Ac NPV相同。以上结果证明Ac NPV可以在蚕蛹体内复制。含HBsAg基因(人乙肝表面抗原)的重组Ac NPV亦可感染家蚕蛹,并能正确表达外源基因。此外,还发现化蛹后第2天的家蚕蛹被注射约5×10~5PFU的Ac NPV,可诱导“人工滞育蛹”现象的发生,在25℃经过30天后蛹仍存活,发育停滞在复眼着色前阶段。     

桉树的一个国内新记录病原菌

本文报道了寄生在雷林一号桉“[Eucalyptus leichou NO:1]”叶片上的一个国内新记录病原菌[Fairmaniella leprosa(Fairman)Petrak & Sydow.]的主要特征,并与已知种作了比较;文中讨论了国内外研究状况,寄主及分布。标本采集于广东省雷州林业局林科所人工桉树林。     

Dynamics ofRhizoctonia solani (black scurf) in successive potato crops

The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3.     

水稻苗田化学除草剂—封闭1号大面积苗田药效示范试验结果

通过多点水稻苗田大面积示范试验结果表明,通化市农科所研制的水稻苗田除草剂“封闭1号”具有除草效果好(90～100％)、安全、使用方便、成本低等特点。1988～1991年,累计推广面积(苗田)达15.6万亩,创造经济效益150万元。     

新型杀蜂螨烟剂蜂乐1号的研究

蜂乐1号烟剂是一种最近研制成功的新型杀蜂螨药剂,其有效成分为溴螨酯.经室内外试验结果,对蜂螨有较强杀伤力,对蜜蜂十分安全.烟剂中的溴螨醋对雅氏大蜂螨的LC_(50)为0.26克/米~3,LC_(95)为2.92克/米~3 对蜜蜂则分别为71.45克/米~3和218.70克/米~3,二者分别相差274.8倍和74.9倍.一平箱蜂群一次用含0.45克有效成分的药,对蜂体上大蜂螨的防除效果平均93%以上.对蜂群每隔5天施药一次,经3次施药后蜂群状况正常.在施药3次后对所取蜜样进行检测,其中溴螨酯的残留量远远低于联合国粮农组织所规定的参考限量.该烟剂适于在蜂箱外施药,比目前国内一般的开箱施药方法提高工效15倍以上,更适用于许多过去不便用药的场合.