Identification of the Chinese Box Orange (Severinia Buxifolia) as an Alternative Host of the Bacterium Causing Citrus Huanglongbing

The Chinese box orange (Severinia buxifolia) was shown by graft-inoculation and psyllid-transmission tests to be an alternative host of the bacterium causing citrus Huanglongbing (HLB). A PCR-based assay for detection of the HLB bacterium (HLBB) was used to monitor HLBB. In graft-inoculation tests, the Chinese box orange (CBO) grafted with HLBB-infected scions of Luchen sweet orange (LSO) were positive for HLBB, 2–3 months after grafting. The back-grafting test demonstrated that HLBB-infected CBO scions could transmit HLBB back to LSO hosts via grafting. In psyllid-transmission tests, psyllids (insect vectors) transmitted HLBB to CBO plants, in which HLBB could be detected 3–4 months after inoculation. Acquisition-access tests of psyllids revealed that HLBB-free psyllids can acquire HLBB from diseased CBO hosts and can transmit HLBB back to the LSO plants. A field survey verified the presence of HLBB-infected CBO plants in the vicinity of citrus orchards. In this paper, CBO is shown to be a susceptible host plant in which HLBB can exist and replicate. It is also a donor plant from which HLBB can be transmitted to citrus hosts by grafting or by psyllid vectors.     

Mitotic disruption by a novel pyrimidine herbicide, NS-245852, in oat (Avena sativa L.) roots

The effects of a novel pyrimidine herbicide, NS-245852 [2-chloro-6-fluorophenyl-4-(trifluoromethyl)thieno[2,3-d]pyrimidine-2-yl-ketone], on mitosis in oat ( Avena sativa L. cv. Zenshin) root tips were investigated by using light and immunofluorescence microscopy. The root growth was strongly inhibited at 10⁻⁷ mol L⁻¹ of NS-245852, and swollen root tips were induced at 5 × 10⁻⁸ mol L⁻¹. As observed by the use of light microscopy, the herbicide produced disrupted mitosis and large polynucleate cells in the meristematic root tissue. These symptoms were similar to those of mitotic disrupter herbicides. The immunofluorescence microscopy studies of the root tip cells treated for 30 min revealed that spindle fibers and the preprophase band were reduced, although kinetochore fibers and the phragmoplast were not affected. Kinetochore fibers remained as small fluorescence spots, and the phragmoplast disappeared after a 3 h treatment. No microtubule arrays were observed by a longer treatment (longer than 3 h). Among the microtubule arrays, spindle fibers and the preprophase band were found to be the most sensitive to the herbicide, whereas kinetochore fibers were the most resistant. The phragmoplast was intermediate. Thus, the primary action of NS-245852 is the inhibition of polymerization of tubulin into microtubules.     

Effect of GRAS compounds on mycelial growth, pectic enzyme activity and disease severity of postharvest pathogens on rambutan (Nephelium lappaceum)

The GRAS compounds cinnamaldehyde (at 30 ppm), acetaldehyde (at 70 ppm), benzalde-hyde (at 50 ppm) and potassium metabisulphite (at 250 ppm) either completely inhibited or significantly reduced thein vitro mycelial growth ofGliocephalotrichum microchlamydosponim,Colletotrichum gloeosporioides andBotryodiplodia theobromae, the causative fungi of brown spot, anthracnose and stem end rot, respectively, of rambutan fruits,Nephelium lappaceum. The four compounds also significantly reduced the severity of all three diseases and the activity of pectic lyase and polygalacturonase enzymes secreted by the three fungi.     

莨菪烷碱防治蔬菜根结线虫药效研究

本文报道了莨菪烷碱（hyoscyamine)防治蔬菜根结线虫（Meloidogyne incognita)效果，该制剂以有效成分用量7.5-10.5g/667m^2对水稀释后穴施，药后150d对线虫防效达73.78%-82.84%，优于3％米乐尔（isazofos)颗粒剂有效成分用量150g/667m^2穴施药后150d的防效65．30％，并有一定刺激生长作用，有较高推广应用价值。     

黑河(额济纳河)下游绿洲生态系统受损与生态保育对策的思考

额济纳绿洲是我国西北干旱区中心的一道生态防线。 2 0世纪后半世纪绿洲生态系统严重受损 ,景观结构急剧恶化 ,黑河的供水量逐年趋于下降 ,由 50年代的 1 2 .2 5亿 M3 下降到 90年代的 7.2 4亿 M3。地下水位也相应地降低 ,土地盐化也在加剧。胡扬与沙枣林面积由3 .45万公顷减少到 2 .2 0万公顷 ,并以老龄林为主。红柳灌丛也减少了 50 % ,并且多已成为稀疏矮化的群落。各种禾草草甸严重退化 ,多已演替成为苦豆子群落。为了维护我国西北的这一生态屏障 ,必须坚持以恢复与保护自然绿洲为主 ,恢复胡杨林、沙枣林及红柳灌丛的覆盖率 ;建立人工草地 ,改善土地覆盖 ,建设自然一人工复合绿洲。在绿洲建设与保育工程中必须保证充分供水 ,调控水盐动态 ,发挥生物一景观多样性的系统耦合效应 ,实现绿洲生态系统健康     

陕西省果树黑星病发生现状及药剂防治策略

苹果、梨黑星病的初侵染发生率高低和发病中心的形成与当年5～6月份降雨量密切相关 ,而流行则与7～8月份降雨量密切相关 ,在防治策略上应重点抓住5～6月份的预报工作 ,药剂施用上应选择保护性杀菌剂及内吸性杀菌剂交替使用 ,在发病初期每隔7d喷药1次 ,连续喷3～4次 ,防效可达80%～85%。     

利用诱导因子抗新疆甜瓜疫霉病的田间效果

用化学诱导因子和灭活的生物诱导因子在田间自然条件下诱导了新疆甜瓜产生对疫霉病的整体抗性。1996年的试验结果表明，用化学诱导因子诱导处理，最高诱抗效果可达79．40％，平均诱抗效果在40％以上；生物诱导因子诱导处理，最高诱抗效果为68．2％，平均诱抗效果为30％以上。1997年的试验基本上验证了1996年的结果，化学诱导因子最高诱抗效果可达60％，平均诱抗效果为35．86％，经t测验分析，诱抗效果显著。     

Effect of pyrethroids on carbohydrate metabolic pathways in common carp,Cyprinus carpio

The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry     

QSAR及其在农药设计中的应用和进展

在介绍定量结构 -活性关系 (QSAR)的基本原理后 ,重点评述了 QSAR各种方法的主要原理、优缺点及其在农药设计中的研究应用进展 ,包括取代基多参数法 (Hansch法 )、Free- Wilson法、分子轨道法 (MO)、距离比较法 (DISCO)、比较分子力场分析法 (Co MFA)、分子模拟法 (MS)、分子对接法 (MD)、人工神经网络法 (ANN)以及 Leapfrog法等。     

杀菌剂银泰防治小麦纹枯病研究

采用室内离体平皿法和田间小区试验测定了植物源农用杀菌剂银泰 (有效成分 :4 -丁酰基苯酚 )对小麦纹枯病菌 Rhizoctonia cerealis生长的抑制作用和对该病害的防治效果。结果表明 ,20%银泰乳油在 0～ 100μg/mL范围内随着浓度提高对小麦纹枯病菌的抑制作用增强 ,其 EC50 和 EC95分别为 36.11和 97.66μg/L;EC95明显小于对照药剂井冈霉素的检测数值 (327.6 4μg/L )。20%银泰乳油 200、400和 800mg/L防治小麦纹枯病的效果分别为 85.8%、89.3%和 92.3% ,与对照药剂甲基硫菌灵 400mg/L的防效 (87.0% )相当。