吐鲁番几种多熟制栽培模式及经济效益

为发挥吐鲁番地区热量资源足、日照时间长、昼夜温差大等优势,经过不断实践总结,推广了多种形式的多熟制间作套种技术,并取得明显的效益和成果,但是尚未打破一年两熟制的基本模式,在一年三熟种植方面,有待进一步去探索。     

玉米叶片衰老相关基因的研究进展

玉米叶片衰老对子粒形成和产量有着重要的影响。大量研究表明,叶片衰老受众多因素影响,造成形态及生理生化的巨大变化,而这些变化均离不开基因的调控作用。本文综述了近年来国内外有关玉米叶片衰老的研究结果,主要包括玉米叶片衰老的生理生化和栽培技术研究及有关玉米叶片衰老的分子生物学研究,以期能为玉米叶片衰老的进一步研究提供参考。     

一株大豆内生拮抗细菌XZ-2的分离和分子鉴定

从大豆植株中分离到一株具有广谱抗菌活性的拮抗细菌,命名为XZ-2。抑菌活性测定显示XZ-2对甘薯黑斑病菌、大蒜叶枯病菌等8种植物病原真菌均具有较强的抑菌活性,抑菌直径均在20 mm以上。采用分光光度法绘制了XZ-2的生长曲线,结果显示该菌株在LB液体摇培12 h时基本达到对数生长期,该生长曲线的绘制为后期XZ-2的活性物质发酵条件优化奠定了基础。通过PCR扩增16Sr DNA片段并采用MEGA 6.0构建系统发育进化树,初步将该菌株鉴定为多粘类芽孢杆菌。     

黄颡鱼池塘主养高效养殖试验

开展黄颡鱼池塘主养,成率活达81.7%、单产达1400kg∕667m2,667m2产值3.133万元,利润0.943万元,投入产出比1∶1.437。     

职业年金多计划模式下投资收益率差异问题浅析

职业年金在多计划模式下,将要面临不同计划下收益率差异的问题,进而引发不同计划内人员对公平性的争议。本文主要探讨这种差异性产生的原因以及进行调整平衡的可行性。     

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

甜樱桃不同砧穗组合成花调控关键基因表达差异研究

为了研究甜樱桃(Prunus avium)成花调控关键基因在不同砧穗组合中表达差异,以甜樱桃‘艳阳’(Sunburst)为接穗,‘ZY-1’(P.cerasus)、马哈利(P.mahaleb)和‘兰丁2号’(P.avium × P.pseudocerasus)为砧木的嫁接组合为试材,调查统计4年干龄不同砧穗组合的总花芽量及开花情况;克隆甜樱桃成花调控网络中EARLY FLOWERING 3(ELF3)、CONSTANS(CO)、FLOWERING LOCUS T(FT)等9个关键作用基因,并利用实时荧光定量PCR对不同砧穗组合接穗的幼叶、叶芽、成龄叶、花芽、叶芽附近叶片叶柄及花芽附近叶片叶柄中这些基因的表达量鉴定。结果显示,艳阳/ZY-1、艳阳/马哈利和艳阳/兰丁2号组合的平均单株花芽量分别为279、288和317朵,不存在明显差异,但不同组合的花期却明显不同,当艳阳/马哈利有72%花芽处于开放期时,艳阳/兰丁2号的花开放比例只有7%,艳阳/ZY-1的花开放比例为49%;此外,PaELF3、PaCO、PaFT等9个成花关键基因,在同一时期不同砧穗组合的接穗组织中表达量存在差异,其中PaAP1在不同砧穗组合的花芽中表达模式与花期规律一致,表明PaAP1与樱桃花期调控密切相关。     

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.     

RBP4基因与动物繁殖性能的相关性

视黄醇结合蛋白4(RBP4)是动物体内一种重要的转运蛋白,它在介导维生素A及其代谢物生理功能的正常发挥中起着不可替代的作用。RBP4是孕体产生的主要蛋白之一,对动物的胚胎发育和繁殖性能有较显著的影响。RBP4的功能障碍会导致维生素A的储存、转运、分布及代谢的异常,进而引发各种疾病,并影响上皮、骨组织的生长、分化与繁殖、胚胎发育。本文综述了RBP4基因的结构与定位,RBP4的生物学功能,以及RBP4基因与动物繁殖性能的关系。     

水稻编码光系统Ⅱ相关捕光色素蛋白Lhcb2基因的克隆与表达分析

捕光复合物蛋白(LHCP)在植物光合作用过程中发挥重要的作用。基于水稻基因组信息,以亚洲栽培稻籼稻品种黄华占、长雄蕊野生稻及其杂种F_1为试验材料,克隆了水稻Lhcb2基因,并对其基因内含子信息、蛋白序列特征、进化树、蛋白结构、基因表达进行分析。结果表明:水稻Lhcb2基因全长880 bp,含有1个长98 bp的内含子。开放阅读框长度为792 bp,编码263个氨基酸;水稻LHCB2与13个其他植物的LHCB氨基酸序列一致性为68.78%。进化树分析显示,水稻LHCB2蛋白与同属禾本科的毛竹和麻竹亲缘关系最近,与低等植物团藻亲缘关系最远。水稻LHCB2蛋白属于亲水性蛋白,其理论分子量为28 495.40,理论等电点为5.62,具有2个无序化区域,无序化比例为30.04%;水稻LHCB2蛋白三级结构有2个β折叠和3个α螺旋组成。Lhcb2基因荧光定量分析结果显示,长雄蕊野生稻和杂交F_1代植株叶片中表达量分别为黄华占的1.22和1.96倍。长雄蕊野生稻中的叶绿素a和b、色素、类胡萝卜素等均高于黄华占及其杂交F_1代。长雄蕊野生稻叶绿素a的含量分别为另2份水稻材料的1.13和1.75倍,叶绿素b含量为1.10和1.60倍,色素含量则为1.13和1.72倍。