Pollen tube growth in crosses between Porteresia coarctata and Oryza sativa

Summary Comparative study with fluorescence microscopy revealed that the pollen tubes of the self-pollinated rice variety BR-9 reached the ovary within 75 min after pollination. In P. coarctata it took 150 min. In the cross between P. coarctata and BR-9, pollen tubes reached the ovary in 165 min but seed setting was not observed. In the cross between BR-9 and P. coarctata, small and deformed pollen tubes were formed and they failed to grow through the stylodium.     

Effects of gamma irradiation on in vitro pollen germination of different Cucumis species

Summary Pollen grains of 14 Cucumis accessions were irradiated with 0, 1, 2, 3 or 4 kGy acute gamma rays and germinated in vitro directly afterwards. Pollen germination was significantly reduced by increasing irradiation dose for all species, except C. melo var. agrestis. Pollen tube growth was generally reduced likewise. Pollen of two C. anguria subspecies was most sensitive to irradiation. Sensitivity of the pollen with respect to pollen tube growth in relation to irradiation dose was inversely related to total DNA amount per nucleus. In vitro germination was not related to DNA amount per nucleus. Results show that the examined Cucumis species, especially C. melo var. agrestis, are sufficiently resistant to irradiation to be used as donor species for in vivo egg cell transformation of the cucumber.     

COMPUTING THE VORTEX ELECTRIC FIELD BY THE SUPERIMPQSITION METHOD OF THE ELEMENT OF MAGNETIC FIELD

In this paper, the vortex electric field is computed. It is caused by magnetic field subject to two dimensional change. This problem can't be solved in general physics. Two examples are listed.     

Evaluation of the High-temperature Tolerance of Floral Organs in Upland Cotton (Gossypium hirsutum L.)

The development of floral organs in upland cotton (Gossypium hirsutum L.) is related to squares and yield formation. The germination percentage of pollen grains, the rate of anther dehiscence and the length of filaments and styles of 11 upland cotton cultivars were determined before and after high-temperature periods by pollen grain culture in vitro. We aimed to analyze the effects of high temperature on pollen germination in styles and on stamen characteristics. The number of pollen tubes in styles and cytological structure of anthers were also tested under simulation of high temperatures by paraffin sectioning of cotton anthers. We found that the germination percentage of pollen grains and rate of anthers dehiscence of Ke 1053, Simian 4 and the male parent of Xiangzamian 3 were greater than those of other cultivars under high field temperatures. There was no significant difference in the length ratio of filaments and styles and filaments length between 11 upland cotton cultivars under high field temperatures. After high-temperature periods in the field, the difference in pollen germination percentage, anther dehiscence, style and filament length between the 11 cultivars was not significant. The number of pollen tubes in Ke 1053, Simian 4 and the male parent of Xiangzamian 3 was greater than those of other cultivars under the high simulation temperature. The number of deformed pollen grains in anthers was also less than those of other cultivars. These results were consistent with the field conditions. This experiment indicated that germination percentage of pollen grains and the rate of anther dehiscence are useful as indicators for screening high-temperature tolerance. The length ratio of filaments and styles and the filaments length could be used as parameters for screening high-temperature tolerance cultivars.     

Barriers to hybridization of Solanum bulbocastanum Dun. and S. Verrucosum Schlechtd. and structural hybridity in their F1 plants

Summary Results of reciprocal crosses between Solanum verrucosum (2n=2x=24) and S. bulbocastanum (2n=2x=24) are described in terms of pollen tube behaviour in styles, of berry and seed set, of fertility and of meiotic behaviour of the F₁ hybrids. Pollen tube growth of S. verrucosum is strongly inhibited in styles of S. bulbocastanum, whereas no inhibition is observed in the reciprocal cross. Therefore S. bulbocastanum x S. verrucosum fails to set berries or seeds, whereas the reciprocal cross produces both berries (54.4% berry set) and seeds (0.3 per berry). Only 14.6% of the seeds germinate. Both the diploid and corresponding tetraploid hybrid plants are vigorous, flower abundantly, have a rather regular meiosis (mainly rod bivalents), but show a high degree of cytoplasmic-genic male sterility. Crossability of the diploid hybrid plants is nil when used as pollen parents and near to zero when used as pistillate parents. The barriers to hybridization of the parent species (unilateral inhibition of pollen tube growth, somatoplastic sterility, cytoplasmic-genic male sterility and structural differences of the parental chromosomes) are discussed and methods are suggested to overcome these barriers.     

基于粒子图像测速技术的液力变矩器涡轮内流场测试与分析

液力变矩器是自动变速器的主要部件,其在乘用车、载重汽车、公共汽车和机车上的应用广泛。液力变矩器内部流动特性影响其外部性能,为了深入研究其内部流动特性,基于粒子图像测速(particle image velocimetry,PIV)技术对液力变矩器涡轮内流场进行试验研究。采用帧频为1 000帧/s的CCD高速相机,在不同工况下(输入与输出的转速比分别取0、0.3、0.5、0.7)采集不同粒子直径、不同粒子浓度下的流动图像,经过图像预处理,对连续两帧图像进行互相关计算,获得涡轮径向切面的速度场和涡量场。通过对流场分布结构和流动区域上复杂流动现象的对比分析,发现投入流场中粒子浓度越高(1 500mL蒸馏水中投入2.4 g粒子)、粒子直径越小(10μm)时,识别并提取流动区域上的流动参数越丰富,流速场和涡量场信息越可靠。液力变矩器内部高梯度流场分布结构和非均匀流速场分布导致出现多尺度涡旋和反向流等复杂流动现象,造成液力变矩器内部流动能量损耗,随转速比提高,涡轮流场结构趋于规律,能耗逐渐降低。该文试验测量与分析结果对于液力变矩器结构优化和性能提升提供了参考。     

杉木花粉和花粉管中微丝骨架

花粉萌发、花粉管生长是植物有性生殖的重要环节,包含许多复杂的生理过程。微丝是细胞骨架重要组成成分,很多研究表明微丝在花粉萌发、花粉管内原生质流动(Justus et al.,2004)、花粉管顶端生长(Li et al.,2001)等方面具有重要的作用。花粉管     

热脱附-气相色谱质谱法测定挥发性有机化合物的研究

运用自动热脱附仪和气相色谱/质谱联用技术,通过对木制品中释放的多种挥发性有机物(VOCs)进行测试,得到较优的测试处理条件.研究发现,该方法的重现性和稳定性较好;较低的采样流速,可有效的降低苯在Tenax TA吸附管中的穿透行为.     

Boron fertilization improves seed yield and harvest index of Camelina sativa L. by affecting source-sink

The impact of soil (1, 2 kg ha^?1) and foliar (100, 200 mg L^?1) boron (B) with control (no B) was evaluated on phenology and yield formation of Camelina each applied at stem elongation and flowering stages. Foliar (200 mg L^?1) or soil B (2 kg ha^?1) resulted in earlier flowering and maturity, increased fruit bearing branches (19.68%), number of siliqua, seeds per siliqua (4.6%), biological yield (15%), seed yield (24%), harvest index (11.4%) and oil contents (23%) than no B. Increased fruit bearing branches, seed filled siliqua or seed numbers, harvest index and oil quality can be attributed to changes in dry matter accumulated of stem with simultaneous increase in siliqua dry weight with foliar or soil applied B. In crux, foliar (200 mg L^?1) or soil applied (2 kg ha^?1) B seems promising to improve seed and oil yield, harvest index of Camelina sativa under B deficient condition.     

单叶省藤组培苗与实生苗的造林效果分析

通过在黄梁木和八宝树林分下设置组培丛芽苗与组培单芽苗、组培单芽苗与实生苗造林试验,对不同苗木的造林效果进行分析.研究结果表明:组培单芽苗的保存率、萌蘖数和萌蘖率总体上高于丛芽苗;丛芽苗造林后1.5 a的母茎长显著大于单芽苗,3~4 a时二者无显著差异,而造林后8 a单芽苗的母茎长超过丛芽苗,二者间存在显著差异;在造林后4 a内丛芽苗的藤丛总茎长明显大于单芽苗,造林后8 a丛芽苗的总茎长略优于单芽苗,但二者之间的差异不显著.组培单芽苗的保存率、萌蘖数和萌蘖率均高于实生苗;两者母茎长和藤丛总茎长在不同年龄时均无显著差异.