橡胶树胶乳全蛋白的提取及双向电泳分析

以橡胶树无性系RRIM600为材料，采用TCA(三氯乙酸)-丙酮沉淀法提取胶乳全蛋白，17 cm pH 5～8固相pH梯度预制干胶条和快速银染法对提取的蛋白进行分离和染色，所得到的电泳图谱背景清晰，横条纹和竖条纹少，且蛋白点数量多。通过本研究获得了高分辨率的胶乳全蛋白双向电泳(2-DE)图谱的方法，为进一步开展橡胶树胶乳全蛋白质组学研究奠定了基础。     

我院中文图书借阅情况统计与分析

通过对安徽经济管理学院图书馆2008—2009学年中文图书借阅情况的统计与分析,以及不同年级学生借阅特点的分析,就如何提高馆藏建设,优化馆藏结构,满足不同读者的阅读需求,提出了合理建议。     

Effect of Fermented Chinese Medicine Preparation by Bacillus subtilis on Growth Performance,Biochemical Indices,Antioxidant Parameters and Ability of Anti-infection of Common Carp

The objective of this study was to evaluate of the growth performance, serum biochemical indices, liver antioxidant parameters and ability of anti-infection with Aeromonas hydrophila by adding the fermented Chinese medicine by Bacillus Subtilis to the carp fish feed, and to find out the appropriate supplemental level of fermented Chinese medicine in diets. Carp fingerling with an initial body weight of (35±0.5) g were set up to 6 experimental diets supplemented with 0 (control group), 0.1‰, 0.5‰, 1‰, 2‰ and 3‰ fermented Chinese medicine for 56 days, respectively. Each group had 30 fish and set up 3 repetitions. After the feeding trail,the fish were challenged with Aeromonas hydrophila to calculate the relative percentage survival (RPS).The results showed as follows:Supplementing fermented traditional Chinese medicine by Bacillus subtilis, the weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), the activities of lysozyme (LSZ), complement 3 (C3),total protein (TP),total anti-oxidation capacity (T-AOC),total superoxide dismutase (T-SOD) and catalase (CAT) in serum of common carp were enhanced at different degrees, while the feed coefficient (FCR)and malondialdehyde (MDA) in serum were declined at different degrees.When the fermented Chinese medicine supplemental levels were 2‰ and 3‰,the effects were significant (P<0.05)(except for C3, ALP and Cr).The experimental results of the virulence of common carp infected with pathogen Aeromonas hydrophila showed that,the fermented herbal could obviously improve the ability of anti-infection, the immune protective rate of the group with 2‰, 3‰ doses of the fermented Chinese medicine could reach 77.23% and 74.63%, respectively. From what had been discussed above, the group with 2‰ and 3‰ doses of the fermented Chinese medicine had significant effect on the growth performance, serum biochemical indices, liver antioxidant parameters and Aeromonas hydrophila anti-infection ability. Considering the cost of adding fermented Chinese medicine, the recommendations for the best additive quantity was 2‰.     

北方蚕业科研协作区第六批桑品种鉴定(陕西点)试验报告

2015—2016年对北方蚕业科研协作区4家单位的9个桑树新品种进行了共同鉴定。本批参鉴品种中,生长势旺、产叶量高、叶质好的品种有鲁插2号、鄂桑2号、陕桑9312、陕桑8826,其中鲁插2号叶片大而厚、发条力强,鄂桑2号节间密、片叶率高。     

Observations on the occurrence and phenology of the invasive elm defoliator Aproceros leucopoda (Hymenoptera: Argidae) in the Czech Republic

Aproceros leucopoda has been found at nine locations in urban areas and in forests in the Czech Republic. Aproceros leucopoda was first reported at a location 700 m a.s.l. in the northern part of the country in 2011. The other locations range in elevation from 150 to 460 m a.s.l. In the current study, A. leucopoda was studied in four plots, where larvae and their zigzag feeding tracks were observed on 10 Ulmus trees located south of Lanžhot (southeastern Czech Republic) in 2014 and 2015. In 2014, feeding tracks and larvae were detected in late May, from 22 June to 13 July, and from 9 August to 6 September, suggesting the occurrence of three generations per year in the southeastern Czech Republic. The southern Czech Republic has been colonized much earlier than other parts of the country because of its proximity to localities with A. leucopoda in Slovakia and Austria. The presence of larvae or evidence of larval feeding was detected on only 2.84% of monitored leaves. We suggest that A. leucopoda endangers solitary elms in urban greening areas more than elms in forests.     

Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature

The paper is a brief introduction of porcine endogenous retrovirus (PERV) innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig, analyzes the PERV-virus gene cloning and sequence, appraises method on cell level, create the platform of infection HEK293 cells research, study on pigs A3F inhibition of PERV, and reveal the innovative research results on the specific molecular genetics in the different strain of PERV, analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV,and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs. It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development.     

岳阳市道路绿地系统物种多样性研究

道路绿化作为城市森林重要的组成部分，对整个城市生态环境的改善起着重要的作用，本文以岳阳市的主道路绿地和街头绿地为例，对岳阳市道路绿地系统物种多样性进行分析研究。     

牡丹嫁接成活率试验研究

进行牡丹嫁接成活率试验,结果表明:嫁接时间早、采用贴接法、选择适应强的品种、以牡丹根作砧木进行嫁接繁殖,在一定程度上能提高嫁接成活率。     

中原经济区建设中农村人口有序转移的政府责任研究

以当前国家主体功能区划分背景下中原经济区建设为背景,在对中原经济区建设中农村人口有序转移进行研究的基础上,提出了各级政府部门推动农村人口有序转移的对策建议。     

试用“气韵生动”分析中国古典园林造园艺术

从“气韵生动”这一绘画美学理论的特殊视角来分析中国古典园林的造园艺术，阐述了“气”和“韵”在中国古典园林的具体体现。说明“气韵生动”是中国古典园林的一个重要特征，从这一角度来研究中国古典园林会给我们带来新的启发。