Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.     

Effect of Manually Preheparinized Syringes on Packed Cell Volume and Total Solids in Blood Samples Collected from American Alligators (Alligator mississippiensis)

The hemodilution effect of manually preheparinized syringes was determined by evaluating the packed cell volume (PCV) and total solids (TS) from blood samples collected from 50 American alligators (Alligator mississippiensis). A volume of 0.2 mL of blood was drawn into 3 syringes of 1 mL: control with no heparin sodium, heparin sodium drawn to 0.1 mL and then expelled, and heparin sodium drawn to 0.2 mL and then expelled. PCV and TS values were determined from each syringe immediately after collection. Mean PCV and TS values were compared between the control and heparin groups using linear mixed modeling. The syringes coated with heparin resulted in a significantly lower mean PCV and TS values when compared with controls, with no significant difference between the heparin groups. This dilution effect was also found to be inconsistent and not accurate from one syringe to another. An adjunct method of obtaining 0.5 mL from already-collected blood samples into 1-mL syringes that were coated with heparin drawn to 0.2 mL and then expelled also showed a significant decrease in PCV and TS values when compared with the control samples, although to a lesser extent. As a result, it cannot be recommended to manually preheparinize syringes when collecting small volume of blood samples from American alligators because significant and unpredictable hemodilution is likely to occur.     

半胱胺对藏绵羊断奶羔羊瘤胃pH值、总脱氢酶及TVFA的影响

试验研究了半胱胺(Cs)对藏绵羊断奶羔羊瘤胃pH值、总脱氢酶及TVFA的影响。将10只(公母各半)80日龄左右、体重平均为(16.00±0.31)kg、装有永久性瘤胃瘘管的藏绵羊断奶羔羊随机分为2组(试验组和对照组),在日粮精料中添加300 mg/(kg·BW)的Cs,隔日添加1次,试验期42 d。正式试验当日及试验第7、14、21、28、35天的7:00、9:00、11:00、13:00、15:00、17:00、19:00、21:00、23:00、1:00、3:00、5:00于颈静脉采血样10 mL,同时采集瘤胃液10 mL,测定瘤胃pH值、总脱氢酶的活性和TVFA。检测结果表明,试验组精料中添加Cs使藏绵羊断奶羔羊瘤胃液pH值升高;血清尿素氮浓度降低,与对照组相比差异极显著(P0.01);瘤胃液总脱氢酶活力提高极其明显(P0.01);整个试验期内,半胱胺对瘤胃TVFA的影响极显著(P0.01)。     

不同山药品种糖含量的比较分析

采用不同的测定方法,对6个不同山药品种中的还原糖、总糖及粗多糖进行测定。结果表明:不同品种间的糖含量存在一定的差异,其中‘明豆子’中的还原糖、总糖和粗多糖的含量最高,含量分别为0.1485%、0.3812%、0.2246%,其余品种不同的组分含量有所差异,综合分析‘明豆子’的市场开发价值最高。     

中华绒螯蟹血淋巴和肝胰腺的总蛋白含量与性早熟的关系

中华绒螯蟹扣蟹性腺发育与肝胰腺和血淋巴的蛋白质浓度相关.性早熟前后,雌雄扣蟹肝胰腺总蛋白浓度分别由(121.17±44.50)mg@g-1和(89.43±21.86)mg@g-1下降至(81.08±5.84)mg@g-1和(70.58±9.90)mg@g-1,雌性扣蟹的肝胰腺占体重的比值也由8.18%降至3.90%.血淋巴总蛋白浓度正常发育的雌性扣蟹为(99.55±12.06)mg@mL-1,明显高于性早熟雌性扣蟹的(72.26±17.87)mg@mL-1;而雄性扣蟹性早熟前后的血淋巴总蛋白浓度无显著差异.肝胰腺总蛋白浓度二秋龄雌蟹高于性早熟雌性扣蟹,但它们之间的血淋巴总蛋白浓度没有显著差异.二秋龄雄蟹的血淋巴和肝胰腺总蛋白浓度与性早熟雄性扣蟹相当,差异不显著.     

Tanjungides A and B: New Antitumoral Bromoindole Derived Compounds from Diazona cf formosa. Isolation and Total Synthesis

Tanjungides A (1) (Z isomer) and B (2) (E isomer), two novel dibrominated indole enamides, have been isolated from the tunicate Diazona cf formosa. Their structures were determined by spectroscopic methods including HRMS, and extensive 1D and 2D NMR. The stereochemistry of the cyclised cystine present in both compounds was determined by Marfey’s analysis after chemical degradation and hydrolysis. We also report the first total synthesis of these compounds using methyl 1H-indole-3-carboxylate as starting material and a linear sequence of 11 chemical steps. Tanjungides A and B exhibit significant cytotoxicity against human tumor cell lines.     

Chemistry and Biology of Bengamides and Bengazoles,Bioactive Natural Products from Jaspis Sponges

Sponges corresponding to the Jaspidae family have proved to be a prolific source of bioactive natural products. Among these, the bengamides and the bengazoles stand out by virtue of their unprecedented molecular architectures and impressive biological profiles, including antitumor, antibiotic and anthelmintic properties. As a consequence, intense research activity has been devoted to these compounds from both chemical and biological standpoints. This review describes in detail the research into these classes of natural products and the benefits they offer in chemistry and biology.     

不同土地利用方式对苏打盐碱土壤氮素垂直分布的影响

供试材料于2012年3月份采集于吉林省西部盐碱地典型代表区白城市镇赉县嘎什根乡,材料选择地块分别为:碱斑地、芦苇地、旱田地(前茬为玉米,采样时也为玉米)、水稻种植1年地、水稻种植5年地、水稻种植10年地(分别用SJ、SL、SH、SS1、SS5、SS10表示)。结果表明:盐碱地不同土地利用方式对土壤氮素空间分布具有一定影响,盐碱水田随着种植年限的增加,土壤全氮、速效氮含量均增加,在种植1~5年内,增加幅度较大,积累的氮素多富集于0~20 cm土层内。     

干燥致密土壤在沙粒流冲击下的磨蚀规律

在土壤风蚀过程中,跃移颗粒对表土的冲击磨蚀是公认的一种重要起尘机制。磨蚀体现了跃移沙与土壤相互作用,受沙粒跃移运动参数和土壤力学性质共同控制。然而,现有风蚀模型尚未充分考虑土壤力学性质的地位与作用,缺少磨蚀速率计算公式,亟需从固体力学角度探讨土壤磨蚀规律。该研究借鉴岩石磨蚀模型,针对含水率小于1.5%、体密度大于1.60×103 kg/m3的干燥致密土壤,提出了代表单位时间内的碰撞次数、土壤抗压强度和杨氏模量、入射沙粒动能和密度等综合效应的磨蚀变量;通过标准压缩试验测得黏土、砂土、壤土、砂质黏土、壤质黏土共5种人工均质土壤的抗压强度和杨氏模量的范围分别为0.823～4.092 MPa和0.043～0.149 GPa;利用自行设计的冲击磨蚀试验得到了干燥致密土壤在沙漠沙颗粒流冲击作用下的磨蚀规律,即磨蚀速率是磨蚀变量的线性函数,其斜率与截距分别为3.27×104和-0.027 kg/s。研究结果有助于恰当描述风蚀模型中的土壤力学性质、准确计算风蚀速率,进而服务于农田防护和水土保持工程,并为中国西北干旱区天然地貌和人工建筑风蚀现象的研究提供理论依据与数据支撑。     

Reduced tillage with residue retention improves soil labile carbon pools and carbon lability and management indices in a seven-year trial with wheat-mung bean-rice rotation

Soil total organic carbon (TOC) is a composite indicator of soil quality with implications for crop production and the regulation of soil ecosystem services. Research reports on the dynamics of TOC as a consequence of soil management practices in subtropical climatic conditions, where microbial carbon (C) loss is high, are very limited. The objective of our study was to evaluate the impact of seven years of continuous tillage and residue management on soil TOC dynamics (quantitative and qualitative) with respect to lability and stratification under an annual wheat-mung bean-rice cropping sequence. Composite soil samples were collected at 0-15 and 15-30 cm depths from a three-replicate split-plot experiment with tillage treatment as the main plots and crop residue levels as the sub-plots. The tillage treatments included conventional tillage (CT) and strip tillage (ST). Residue levels were high residue level (HR), 30% of the plant height, and low residue level (LR), 15%. In addition to TOC, soil samples were analyzed for particulate organic C (POC), permanganate oxidizable C (POXC), basal respiration (BR), specific maintenance respiration rate (qCO₂), microbial biomass C (MBC), potentially mineralizable C (PMC), and TOC lability and management indices. The ST treatment significantly increased the TOC and labile C pools at both depths compared with the CT treatment, with the effect being more pronounced in the surface layer. The HR treatment increased TOC and labile C pools compared with the LR treatment. The ST + HR treatment showed significant increases in MBC, metabolic quotient (qR), C pool index (CPI), C lability index (CL_I), and C management index (CMI), indicating improved and efficient soil biological activities in such systems compared with the CT treatment. Similarly, the stratification values, a measure of soil quality improvement, for POC and MBC were > 2, indicating improved soil quality in the ST + HR treatment compared with the CT treatment. The ST + HR treatment not only significantly increased the contents of TOC pools, but also their stocks. The CMI was correlated with qCO₂, BR, and MBC, suggesting that these are sensitive indicators of early changes in TOC. The qCO₂ was significantly higher in the CT + LR treatment and negatively correlated with MBC and CMI, indicating a biologically stressed soil condition in this treatment. Our findings highlight that medium-term reduced tillage with HR management has profound consequences on soil TOC quality and dynamics as mediated by alterations in labile C pools.